Trypanosoma cruzi Infection Induces a Global Host Cell Response in Cardiomyocytes

ABSTRACTChagas' disease, caused by the hemoflagellate protozoanTrypanosoma cruzi, affects millions of people in South and Central America. Chronic chagasic cardiomyopathy, the most devastating manifestation of this disease, occurs in approximately one-third of infected individuals. Events associated with the parasite's tropism for and invasion of cardiomyocytes have been the focus of intense investigation in recent years. In the present study, we use murine microarrays to investigate the cellular response caused by invasion of primary murine cardiomyocytes byT. cruzitrypomastigotes. These studies identified 353 murine genes that were differentially expressed during the early stages of invasion and infection of these cells. Genes associated with the immune response, inflammation, cytoskeleton organization, cell-cell and cell-matrix interactions, apoptosis, cell cycle, and oxidative stress are among those affected during the infection. Our data indicate thatT. cruziinduces broad modulations of the host cell machinery in ways that provide insight into how the parasite survives, replicates, and persists in the infected host and ultimately defines the clinical outcome of the infection.

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Use of F9 teratocarcinoma STEM cells to study cell-matrix interactions in the early mouse embryo

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123416 ◽

1992 ◽

Vol 50 (1) ◽

pp. 602-603

Author(s):

Marc Lenburg ◽

Rulang Jiang ◽

Lengya Cheng ◽

Laura Grabel

Keyword(s):

Mouse Embryo ◽

Inner Cell Mass ◽

Cell Mass ◽

Cell Types ◽

Embryoid Bodies ◽

F9 Cells ◽

Cell Matrix ◽

Matrix Interactions ◽

Cell Matrix Interactions ◽

F9 Teratocarcinoma

We are interested in defining the cell-cell and cell-matrix interactions that help direct the differentiation of extraembryonic endoderm in the peri-implantation mouse embryo. At the blastocyst stage the mouse embryo consists of an outer layer of trophectoderm surrounding the fluid-filled blastocoel cavity and an eccentrically located inner cell mass. On the free surface of the inner cell mass, facing the blastocoel cavity, a layer of primitive endoderm forms. Primitive endoderm then generates two distinct cell types; parietal endoderm (PE) which migrates along the inner surface of the trophectoderm and secretes large amounts of basement membrane components as well as tissue-type plasminogen activator (tPA), and visceral endoderm (VE), a columnar epithelial layer characterized by tight junctions, microvilli, and the synthesis and secretion of α-fetoprotein. As these events occur after implantation, we have turned to the F9 teratocarcinoma system as an in vitro model for examining the differentiation of these cell types. When F9 cells are treated in monolayer with retinoic acid plus cyclic-AMP, they differentiate into PE. In contrast, when F9 cells are treated in suspension with retinoic acid, they form embryoid bodies (EBs) which consist of an outer layer of VE and an inner core of undifferentiated stem cells. In addition, we have established that when VE containing embryoid bodies are plated on a fibronectin coated substrate, PE migrates onto the matrix and this interaction is inhibited by RGDS as well as antibodies directed against the β1 integrin subunit. This transition is accompanied by a significant increase in the level of tPA in the PE cells. Thus, the outgrowth system provides a spatially appropriate model for studying the differentiation and migration of PE from a VE precursor.

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Cell matrix interactions in asthma

Clinical & Experimental Allergy ◽

10.1046/j.1365-2222.1997.d01-423.x ◽

1997 ◽

Vol 27 (1) ◽

pp. 22-27

Author(s):

K. GOLDRING ◽

J. A. WARNER

Keyword(s):

Cell Matrix ◽

Matrix Interactions ◽

Cell Matrix Interactions

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Cell-Matrix Interactions in Breast Carcinoma Invasion

10.21236/ada391446 ◽

2000 ◽

Author(s):

Anna M. Curatola

Keyword(s):

Breast Carcinoma ◽

Cell Matrix ◽

Matrix Interactions ◽

Cell Matrix Interactions

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A computational framework for modeling cell–matrix interactions in soft biological tissues

Biomechanics and Modeling in Mechanobiology ◽

10.1007/s10237-021-01480-2 ◽

2021 ◽

Author(s):

Jonas F. Eichinger ◽

Maximilian J. Grill ◽

Iman Davoodi Kermani ◽

Roland C. Aydin ◽

Wolfgang A. Wall ◽

...

Keyword(s):

Soft Tissues ◽

Three Dimensional ◽

Biological Tissues ◽

Computational Framework ◽

Cell Matrix ◽

Physiological Processes ◽

Matrix Interactions ◽

Mechanical Homeostasis ◽

Cell Matrix Interactions ◽

Short Time

AbstractLiving soft tissues appear to promote the development and maintenance of a preferred mechanical state within a defined tolerance around a so-called set point. This phenomenon is often referred to as mechanical homeostasis. In contradiction to the prominent role of mechanical homeostasis in various (patho)physiological processes, its underlying micromechanical mechanisms acting on the level of individual cells and fibers remain poorly understood, especially how these mechanisms on the microscale lead to what we macroscopically call mechanical homeostasis. Here, we present a novel computational framework based on the finite element method that is constructed bottom up, that is, it models key mechanobiological mechanisms such as actin cytoskeleton contraction and molecular clutch behavior of individual cells interacting with a reconstructed three-dimensional extracellular fiber matrix. The framework reproduces many experimental observations regarding mechanical homeostasis on short time scales (hours), in which the deposition and degradation of extracellular matrix can largely be neglected. This model can serve as a systematic tool for future in silico studies of the origin of the numerous still unexplained experimental observations about mechanical homeostasis.

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Decellularized Porcine Cartilage Scaffold; Validation of Decellularization and Evaluation of Biomarkers of Chondrogenesis

International Journal of Molecular Sciences ◽

10.3390/ijms22126241 ◽

2021 ◽

Vol 22 (12) ◽

pp. 6241

Author(s):

Roxanne N. Stone ◽

Stephanie M. Frahs ◽

Makenna J. Hardy ◽

Akina Fujimoto ◽

Xinzhu Pu ◽

...

Keyword(s):

Cellular Response ◽

Cell Attachment ◽

The United States ◽

Culture Conditions ◽

Surgical Approaches ◽

Cell Matrix ◽

Cartilage Restoration ◽

Cell Matrix Interactions ◽

Decellularized Scaffold ◽

Extracellular Matrix Scaffold

Osteoarthritis is a major concern in the United States and worldwide. Current non-surgical and surgical approaches alleviate pain but show little evidence of cartilage restoration. Cell-based treatments may hold promise for the regeneration of hyaline cartilage-like tissue at the site of injury or wear. Cell–cell and cell–matrix interactions have been shown to drive cell differentiation pathways. Biomaterials for clinically relevant applications can be generated from decellularized porcine auricular cartilage. This material may represent a suitable scaffold on which to seed and grow chondrocytes to create new cartilage. In this study, we used decellularization techniques to create an extracellular matrix scaffold that supports chondrocyte cell attachment and growth in tissue culture conditions. Results presented here evaluate the decellularization process histologically and molecularly. We identified new and novel biomarker profiles that may aid future cartilage decellularization efforts. Additionally, the resulting scaffold was characterized using scanning electron microscopy, fluorescence microscopy, and proteomics. Cellular response to the decellularized scaffold was evaluated by quantitative real-time PCR for gene expression analysis.

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