Characterization of the Highly Regulated Antigen BBA05 in the Enzootic Cycle of Borrelia burgdorferi

ABSTRACT Dramatic alteration of surface lipoprotein profiles is a key strategy that Borrelia burgdorferi, the Lyme disease pathogen, has evolved for adapting to the diverse environments of arthropod and mammalian hosts. Several of these differentially expressed lipoproteins have been shown to play important roles in the enzootic cycle of B. burgdorferi. The BBA05 protein is a previously identified putative lipoprotein (P55 or S1 antigen) that elicits antibody responses in mammals. Recent microarray analyses indicate that the BBA05 gene is differentially expressed by many environmental factors, including temperature. However, the role of the BBA05 protein in the life cycle of B. burgdorferi has not been elucidated. Here we show that expression of the BBA05 gene was exclusively induced in feeding nymphal ticks during the spirochetal transmission from ticks to mammals. Upon generating a BBA05 mutant in an infectious strain of B. burgdorferi, we showed that the BBA05 mutant remained capable of establishing infection in mice, being acquired by ticks, persisting through tick molting, and reinfecting new mammalian hosts. These results indicate that, despite being a highly conserved and regulated antigen, the BBA05 protein has a nonessential role in the transmission cycle of B. burgdorferi, at least in the animal model.

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Genome-Wide Identification and Characterization of Long Noncoding RNAs Involved in Chinese Wheat Mosaic Virus Infection of Nicotiana benthamiana

Biology ◽

10.3390/biology10030232 ◽

2021 ◽

Vol 10 (3) ◽

pp. 232

Author(s):

Weiran Zheng ◽

Haichao Hu ◽

Qisen Lu ◽

Peng Jin ◽

Linna Cai ◽

...

Keyword(s):

Virus Infection ◽

Mosaic Virus ◽

Nicotiana Benthamiana ◽

Noncoding Rnas ◽

Long Noncoding Rnas ◽

Differentially Expressed ◽

Genome Wide ◽

Chinese Wheat

Recent studies have shown that a large number of long noncoding RNAs (lncRNAs) can regulate various biological processes in animals and plants. Although lncRNAs have been identified in many plants, they have not been reported in the model plant Nicotiana benthamiana. Particularly, the role of lncRNAs in plant virus infection remains unknown. In this study, we identified lncRNAs in N. benthamiana response to Chinese wheat mosaic virus (CWMV) infection by RNA sequencing. A total of 1175 lncRNAs, including 65 differentially expressed lncRNAs, were identified during CWMV infection. We then analyzed the functions of some of these differentially expressed lncRNAs. Interestingly, one differentially expressed lncRNA, XLOC_006393, was found to participate in CWMV infection as a precursor to microRNAs in N. benthamiana. These results suggest that lncRNAs play an important role in the regulatory network of N. benthamiana in response to CWMV infection.

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Identification of Long Noncoding RNA Associated ceRNA Networks in Rosacea

BioMed Research International ◽

10.1155/2020/9705950 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13

Author(s):

Lian Wang ◽

Ruifeng Lu ◽

Yujia Wang ◽

Xiaoyun Wang ◽

Dan Hao ◽

...

Keyword(s):

Noncoding Rna ◽

Molecular Mechanisms ◽

Functional Enrichment ◽

Differentially Expressed ◽

Control Group ◽

Group 13 ◽

Regulatory Processes ◽

Coexpression Networks

Rosacea is a chronic and relapsing inflammatory cutaneous disorder with highly variable prevalence worldwide that adversely affects the health of patients and their quality of life. However, the molecular characterization of each rosacea subtype is still unclear. Furthermore, little is known about the role of long noncoding RNAs (lncRNAs) in the pathogenesis or regulatory processes of this disorder. In the current study, we established lncRNA-mRNA coexpression networks for three rosacea subtypes (erythematotelangiectatic, papulopustular, and phymatous) and performed their functional enrichment analyses using Gene Onotology, KEGG, GSEA, and WGCNA. Compared to the control group, 13 differentially expressed lncRNAs and 525 differentially expressed mRNAs were identified in the three rosacea subtypes. The differentially expressed genes identified were enriched in four signaling pathways and the GO terms found were associated with leukocyte migration. In addition, we found nine differentially expressed lncRNAs in all three rosacea subtype-related networks, including NEAT1 and HOTAIR, which may play important roles in the pathology of rosacea. Our study provided novel insights into lncRNA-mRNA coexpression networks to discover the molecular mechanisms involved in rosacea development that can be used as future targets of rosacea diagnosis, prevention, and treatment.

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Reservoir Role of Lizard Psammodromus algirus in Transmission Cycle of Borrelia burgdorferi Sensu Lato (Spirochaetaceae) in Tunisia

Journal of Medical Entomology ◽

10.1603/0022-2585(2006)43[737:rrolpa]2.0.co;2 ◽

2006 ◽

Vol 43 (4) ◽

pp. 737-742 ◽

Cited By ~ 22

Author(s):

Najla Dsouli ◽

Hend Younsi-kabachii ◽

Danièle Postic ◽

Said Nouira ◽

Lise Gern ◽

...

Keyword(s):

Borrelia Burgdorferi ◽

Transmission Cycle ◽

Psammodromus Algirus

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Human IAPP/amylin overproducing transgenic mice: Characterization of an animal model to study the role of IAPP in the pathogenesis of Type 2 diabetes

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-0029-1211891 ◽

2009 ◽

Vol 105 (S 03) ◽

pp. 69-69

Author(s):

J. W. M. Höppener ◽

C. Oosterwijk ◽

G. I. Shulman ◽

J. C. Jiménez-Chillarón ◽

J. J. Guinovart ◽

...

Keyword(s):

Type 2 Diabetes ◽

Animal Model ◽

Transgenic Mice

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The role of the sand lizard (Lacerta agilis) in the transmission cycle of Borrelia burgdorferi sensu lato

International Journal of Medical Microbiology ◽

10.1016/j.ijmm.2008.03.005 ◽

2008 ◽

Vol 298 ◽

pp. 161-167 ◽

Cited By ~ 23

Author(s):

Viktória Majláthová ◽

Igor Majláth ◽

Martin Hromada ◽

Piotr Tryjanowski ◽

Martin Bona ◽

...

Keyword(s):

Borrelia Burgdorferi ◽

Borrelia Burgdorferi Sensu Lato ◽

Transmission Cycle ◽

Sand Lizard ◽

Lacerta Agilis

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Atg16L1 as a Novel Biomarker and Autophagy Gene for Diabetic Retinopathy

Journal of Diabetes Research ◽

10.1155/2021/5398645 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Xinxiao Gao ◽

Yunhui Du ◽

Wayne Bond Lau ◽

Yu Li ◽

Siquan Zhu ◽

...

Keyword(s):

Diabetic Retinopathy ◽

Real Time ◽

Potential Role ◽

Critical Role ◽

Differentially Expressed ◽

Chain Reactions ◽

Polymerase Chain Reactions ◽

Polymerase Chain ◽

Microarray Analyses

Objective. Accumulating evidence suggests the critical role of autophagy in the pathogenesis of diabetic retinopathy (DR). In the current study, we aim to identify autophagy genes involved in DR via microarray analyses. Methods. Gene microarrays were performed to identify differentially expressed lncRNAs/mRNAs between normal and DR retinas. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of lncRNA-coexpressed mRNAs were used to determine the related pathological pathways and biological modules. Real-time polymerase chain reactions (PCR) were conducted to validate the microarray analyses. Results. A total of 2474 significantly dysregulated lncRNAs and 959 differentially expressed mRNAs were identified in the retina of DR. Based upon Signalnet analysis, Bcl2, Gabarapl2, Atg4c, and Atg16L1 participated the process of cell death in DR. Moreover, real-time PCR revealed significant upregulation of Atg16L1. Conclusion. This study indicated the importance and potential role of Atg16L1, one of the autophagy genes, as a biomarker in DR development and progression.

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Corrigendum: Role of HK2 in the Enzootic Cycle of Borrelia burgdorferi

Frontiers in Medicine ◽

10.3389/fmed.2021.668709 ◽

2021 ◽

Vol 8 ◽

Author(s):

Qiang Liu ◽

Haijun Xu ◽

Yan Zhang ◽

Jing Yang ◽

Jimei Du ◽

...

Keyword(s):

Borrelia Burgdorferi ◽

Enzootic Cycle

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Comparative Transcriptomic and Lipidomic Analyses of Human Male and Female Meibomian Glands Reveal Common Signature Genes of Meibogenesis

International Journal of Molecular Sciences ◽

10.3390/ijms20184539 ◽

2019 ◽

Vol 20 (18) ◽

pp. 4539 ◽

Cited By ~ 4

Author(s):

Igor A. Butovich ◽

Nita Bhat ◽

Jadwiga C. Wojtowicz

Keyword(s):

Expression Patterns ◽

Cholesteryl Esters ◽

Lipid Profiling ◽

Signature Genes ◽

Ocular Disorders ◽

Meibomian Glands ◽

Microarray Analyses

Meibum is a lipid secretion that is produced by holocrine Meibomian glands (MGs). MGs are a specialized type of sebaceous glands that are embedded in the human eyelids. Chemically, meibum and sebum are different. A detailed characterization of lipidome and transcriptome of MG is required to deconvolute a complex and poorly characterized array of biosynthetic reactions (termed meibogenesis) that lead to formation of meibum. Changes in the composition and quality of meibum have been linked to various ocular disorders, some of which are more prevalent in males, while others in females. To establish the role of gender in meibogenesis in humans, we characterized MG transcriptomes and lipidomes of females and males, and identified signature genes of meibogenesis in both genders. Specimens of MG tissues were subjected to mRNA microarray analyses. Chemical composition of meibum samples was assessed chromatographically and mass spectrometrically. Both targeted and untargeted approaches were used. About 290 signature genes of meibogenesis were identified. The analyses of their expression patterns demonstrated no major differences between the genders. Lipid profiling of major classes of meibomian lipids, such as wax esters, cholesteryl esters, free cholesterol, (O)-acylated omega-hydroxy fatty acids (OAHFA), cholesteryl esters of OAHFA, and triacylglycerols, also demonstrated only minor (and random) differences in these lipids. The results of transcriptomic analyses correlated well with lipidomic data. Taken together, our data imply that in males and females, meibogenesis proceeds in a similar fashion, yielding secretions with similar, highly conserved, compositions. This finding is important for designing novel, gender-independent diagnostic and therapeutic approaches to various MG-related diseases and pathological conditions.

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