scholarly journals Adhesion of Entamoeba histolytica trophozoites to human erythrocytes.

1982 ◽  
Vol 37 (1) ◽  
pp. 281-285 ◽  
Author(s):  
R López-Revilla ◽  
R Cano-Mancera
Keyword(s):  
Entamoeba Histolytica ◽  
Human Erythrocytes

scholarly journals Entamoeba histolytica Phagocytosis of Human Erythrocytes Involves PATMK, a Member of the Transmembrane Kinase Family

2008 ◽  
Vol 4 (1) ◽  
pp. e8 ◽  
Author(s):  
Douglas R Boettner ◽  
Christopher D Huston ◽  
Alicia S Linford ◽  
Sarah N Buss ◽  
Eric Houpt ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Human Erythrocytes ◽  
Kinase Family

scholarly journals Crystal Structure of Calcium Binding Protein-5 from Entamoeba histolytica and Its Involvement in Initiation of Phagocytosis of Human Erythrocytes

2014 ◽  
Vol 10 (12) ◽  
pp. e1004532 ◽  
Author(s):  
Sanjeev Kumar ◽  
Saima Aslam ◽  
Mohit Mazumder ◽  
Pradeep Dahiya ◽  
Aruna Murmu ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Entamoeba Histolytica ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Human Erythrocytes

scholarly journals Dynamism of PI4-Phosphate during Interactions with Human Erythrocytes in Entamoeba histolytica

2020 ◽  
Vol 8 (7) ◽  
pp. 1050
Author(s):  
Natsuki Watanabe ◽  
Kumiko Nakada-Tsukui ◽  
Tomohiko Maehama ◽  
Tomoyoshi Nozaki
Keyword(s):  
Entamoeba Histolytica ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Protozoan Parasite ◽  
Human Erythrocytes ◽  
Metabolic Labeling ◽  
Intestinal Protozoan ◽  
Vesicular Traffic ◽  
Cellular Events ◽  
Phosphatidylinositol Phosphates

Phosphatidylinositol phosphates (PIPs) are involved in many cellular events as important secondary messengers. In Entamoeba histolytica, a human intestinal protozoan parasite, virulence-associated mechanisms such as cell motility, vesicular traffic, trogo- and phagocytosis are regulated by PIPs. It has been well established that PI3P, PI4P, and PI(3,4,5)P3 play specific roles during amoebic trogo- and phagocytosis. In the present study, we demonstrated the nuclear localization of PI4P in E. histolytica trophozoites in steady state with immunofluorescence imaging and immunoelectron microscopy, using anti-PI4P antibodies and PI4P biosensors [substrate of the Icm/ Dot type IV secretion system (SidM)]. We further showed that the nuclear PI4P decreased after a co-culture with human erythrocytes or Chinese hamster ovary (CHO) cells. However, concomitant changes in the localization and the amount of PI(4,5)P2, which is the expected major metabolized (phosphorylated) product of PI4P, were not observed. This phenomenon was specifically caused by whole or ghost erythrocytes and CHO cells, but not artificial beads. The amount of PIP2 and PIP, biochemically estimated by [32P]-phosphate metabolic labeling and thin layer chromatography, was decreased upon erythrocyte adherence. Altogether, our data indicate for the first time in eukaryotes that erythrocyte attachment leads to the metabolism of nuclear PIPs, and metabolites other than PI(4,5)P2 may be involved in the regulation of downstream cellular events such as cytoskeleton rearrangement or transcriptional regulation.

scholarly journals Surface properties of Entamoeba: increased rates of human erythrocyte phagocytosis in pathogenic strains.

1978 ◽  
Vol 148 (5) ◽  
pp. 1137-1143 ◽  
Author(s):  
D Trissl ◽  
A Martínez-Palomo ◽  
M de la Torre ◽  
R de la Hoz ◽  
E Pérez de Suárez
Keyword(s):  
Surface Properties ◽  
Entamoeba Histolytica ◽  
Human Erythrocyte ◽  
Comparative Studies ◽  
Phagocytic Activity ◽  
Human Erythrocytes ◽  
High Rate ◽  
Parasitic Protozoan ◽  
Microscopic Evaluation

The assertion that ingestion of human erythrocytes is restricted to invasive strains of Entamoeba histolytica has not been evaluated previously by comparative studies. In this report we describe the in vitro ingestion of human erythrocytes by pathogenic and nonpathogenic Entamoeba. Microscopic evaluation of erythrophagocytosis by eight different Entamoeba grown in culture revealed that strains of E. histolytica isolated from cases of human dysentery show a much higher rate of erythrocyte ingestion than nonpathogenic strains. However, all strains are able to phagocytize erythrocytes. The extremely high rate of phagocytic activity shown by pathogenic E. histolytica could be one of the properties related to the pathogenicity of this parasitic protozoan.

Erythrophagocytosis by Entamoeba histolytica.- Ultrastructural Study

1972 ◽  
Vol 30 ◽  
pp. 156-157 ◽  
Author(s):  
Norberto Treviño ◽  
Alfredo Feria-Velasco ◽  
I. Ruiz de Chávez
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Entamoeba Histolytica ◽  
Ultrastructural Study ◽  
Saline Solution ◽  
Physiological Saline ◽  
Ultrastructural Level ◽  
Hot Plate ◽  
Physiological Saline Solution ◽  
Axenic Conditions

Although erythrophagocytosis by various species of Entamoeba is a well known phenomenon this has not yet been studied in detail at the ultrastructural level. The present work deals with the description of the incorporation process of erythrocytes by trophozoites of E. histolytica. For this study, trophozoites of E. histolytica, HK-9:NIH strain cultured in axenic conditions and washed human erythrocytes were placed on a hot plate at 37°C in physiological saline solution. After 5 minutes, 2.5% glutarldehyde was added and the samples were processed according to conventional techniques for electron microscopy.Based upon light microscopy studies on living trophozoites in contact with erythrocytes, it seems that erythrophagocytosis only takes place in one pole of the parasite.

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

Elemental analysis of human erythrocytes

1989 ◽  
Vol 47 ◽  
pp. 242-243
Author(s):  
S. A. Livesey ◽  
A. A. del Campo ◽  
E. S. Griffey ◽  
D. Ohlmer ◽  
T. Schifani ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Elemental Analysis ◽  
Human Erythrocyte ◽  
Spectroscopic Analysis ◽  
Model System ◽  
Human Erythrocytes ◽  
List Type ◽  
Chemical Fixation ◽  
Ethanol Dehydration ◽  
Lowicryl K4m

The aim of this study is to compare methods of sample preparation for elemental analysis. The model system which is used is the human erythrocyte. Energy dispersive spectroscopic analysis has been previously reported for cryofixed and cryosectioned erythrocytes. Such work represents the reference point for this study. The use of plastic embedded samples for elemental analysis has also been documented. The work which is presented here is based on human erythrocytes which have been either chemically fixed and embedded or cryofixed and subsequently processed by a variety of techniques which culminated in plastic embedded samples.Heparinized and washed erythrocytes were prepared by the following methods for this study :(1). Chemical fixation in 4% paraformaldehyde/0.25% glutaraldehyde/0.2 M sucrose in 0.1 M Na cacodylate, pH 7.3 for 30 min, followed by ethanol dehydration, infiltration and embedding in Lowicryl K4M at -20° C.

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