scholarly journals Purification and immunochemical properties of Escherichia coli B polysaccharide cross-reacting with Salmonella typhi Vi antigen: preliminary evidence for cross-reaction of the polysaccharide with Escherichia coli K1 antigen.

1983 ◽  
Vol 41 (1) ◽  
pp. 224-231 ◽  
Author(s):  
B Szewczyk ◽  
A Taylor
1980 ◽  
Vol 30 (3) ◽  
pp. 661-667
Author(s):  
B Szewczyk ◽  
A Taylor

Two new antigenic acidic polysaccharides, Vi-P and Vi-C, were isolated from the microcapsule of Salmonella typhi by a very mild procedure. The antigens were purified to serological homogeneity, and it was found that each of them shares with Vi antigen one of its two serological determinants (a different one in each case). One of the antigens Vi-C, was also isolated from Escherichia coli B, which is not a Vi-producing strain.


Author(s):  
Manfred E. Bayer

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.


1966 ◽  
Vol 241 (13) ◽  
pp. 3090-3096
Author(s):  
Sally E. Jorgensen ◽  
James F. Koerner

1970 ◽  
Vol 245 (14) ◽  
pp. 3708-3715 ◽  
Author(s):  
Peter P.K. Ho ◽  
Elizabeth B. Milikin ◽  
Jesse L. Bobbitt ◽  
Edward L. Grinnan ◽  
Philip J. Burck ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document