Serum antibodies to Porphyromonas gingivalis block the prostaglandin E2 response to lipopolysaccharide by mononuclear cells.

Apical periodontitis is an inflammatory disease of microbial etiology. It has been suggested that endodontic bacterial DNA might translocate to distant organs via blood vessels, but no studies have been conducted. We aimed first to explore overall extraradicular infection, as well as specifically by Porphyromonas spp; and their potential to translocate from infected root canals to blood through peripheral blood mononuclear cells. In this cross-sectional study, healthy individuals with and without a diagnosis of apical periodontitis with an associated apical lesion of endodontic origin (both, symptomatic and asymptomatic) were included. Apical lesions (N=64) were collected from volunteers with an indication of tooth extraction. Intracanal samples (N=39) and respective peripheral blood mononuclear cells from apical periodontitis (n=14) individuals with an indication of endodontic treatment, as well as from healthy individuals (n=14) were collected. The detection frequencies and loads (DNA copies/mg or DNA copies/μL) of total bacteria, Porphyromonas endodontalis and Porphyromonas gingivalis were measured by qPCR. In apical lesions, the detection frequencies (%) and median bacterial loads (DNA copies/mg) respectively were 70.8% and 4521.6 for total bacteria; 21.5% and 1789.7 for Porphyromonas endodontalis; and 18.4% and 1493.9 for Porphyromonas gingivalis. In intracanal exudates, the detection frequencies and median bacterial loads respectively were 100% and 21089.2 (DNA copies/μL) for total bacteria, 41% and 8263.9 for Porphyromonas endodontalis; and 20.5%, median 12538.9 for Porphyromonas gingivalis. Finally, bacteria were detected in all samples of peripheral blood mononuclear cells including apical periodontitis and healthy groups, though total bacterial loads (median DNA copies/μL) were significantly higher in apical periodontitis (953.6) compared to controls (300.7), p<0.05. Porphyromonas endodontalis was equally detected in both groups (50%), but its bacterial load tended to be higher in apical periodontitis (262.3) than controls (158.8), p>0.05; Porphyromonas gingivalis was not detected. Bacteria and specifically Porphyromonas spp. were frequently detected in endodontic canals and apical lesions. Also, total bacteria and Porphyromonas endodontalis DNA were detected in peripheral blood mononuclear cells, supporting their plausible role in bacterial systemic translocation.

Download Full-text

Induction of Prostaglandin E2 by Porphyromonas gingivalis in Human Dental Pulp Cells

International Journal of Oral Biology ◽

10.11620/ijob.2017.42.4.149 ◽

2017 ◽

Vol 42 (4) ◽

pp. 149-153

Author(s):

So-Hee Kim ◽

◽

Yun-Woong Paek ◽

In-Chol Kang

Keyword(s):

Prostaglandin E2 ◽

Porphyromonas Gingivalis ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells

Download Full-text

The skin fungus-induced Th1- and Th2-related cytokine, chemokine and prostaglandin E2 production in peripheral blood mononuclear cells from patients with atopic dermatitis and psoriasis vulgaris

Clinical & Experimental Allergy ◽

10.1046/j.1365-2745.2002.01459.x ◽

2002 ◽

Vol 32 (8) ◽

pp. 1243-1250 ◽

Cited By ~ 44

Author(s):

N. Kanda ◽

K. Tani ◽

U. Enomoto ◽

K. Nakai ◽

S. Watanabe

Keyword(s):

Atopic Dermatitis ◽

Prostaglandin E2 ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Psoriasis Vulgaris ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Th1 And Th2

Download Full-text

Lipopolysaccharides from Periodontopathic Bacteria Porphyromonas gingivalis and Capnocytophaga ochracea Are Antagonists for Human Toll-Like Receptor 4

Infection and Immunity ◽

10.1128/iai.70.1.218-225.2002 ◽

2002 ◽

Vol 70 (1) ◽

pp. 218-225 ◽

Cited By ~ 124

Author(s):

Atsutoshi Yoshimura ◽

Takashi Kaneko ◽

Yoshifumi Kato ◽

Douglas T. Golenbock ◽

Yoshitaka Hara

Keyword(s):

Porphyromonas Gingivalis ◽

Mononuclear Cells ◽

Human Peripheral Blood ◽

Periodontal Diseases ◽

Chinese Hamster ◽

Nuclear Factor Κb ◽

Peripheral Blood Mononuclear ◽

Periodontopathic Bacteria ◽

Cd25 Expression ◽

Freeze Dried

ABSTRACT Toll-like receptors (TLRs) 2 and 4 have recently been identified as possible signal transducers for various bacterial ligands. To investigate the roles of TLRs in the recognition of periodontopathic bacteria by the innate immune system, a Chinese hamster ovary (CHO) nuclear factor-κB (NF-κB)-dependent reporter cell line, 7.7, which is defective in both TLR2- and TLR4-dependent signaling pathways was transfected with human CD14 and TLRs. When the transfectants were exposed to freeze-dried periodontopathic bacteria, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Capnocytophaga ochracea, and Fusobacterium nucleatum, and a non-oral bacterium, Escherichia coli, all species of the bacteria induced NF-κB-dependent CD25 expression in 7.7/huTLR2 cells. Although freeze-dried A. actinomycetemcomitans, F. nucleatum, and E. coli also induced CD25 expression in 7.7/huTLR4 cells, freeze-dried P. gingivalis did not. Similarly, lipopolysaccharides (LPS) extracted from A. actinomycetemcomitans, F. nucleatum, and E. coli induced CD25 expression in 7.7/huTLR4 cells, but LPS from P. gingivalis and C. ochracea did not. Furthermore, LPS from P. gingivalis and C. ochracea attenuated CD25 expression in 7.7/huTLR4 cells induced by repurified LPS from E. coli. LPS from P. gingivalis and C. ochracea also inhibited the secretion of interleukin-6 (IL-6) from U373 cells, the secretion of IL-1β from human peripheral blood mononuclear cells, and ICAM-1 expression in human gingival fibroblasts induced by repurified LPS from E. coli. These findings indicated that LPS from P. gingivalis and C. ochracea worked as antagonists for human TLR4. The antagonistic activity of LPS from these periodontopathic bacteria may be associated with the etiology of periodontal diseases.

Download Full-text