In Vivo Transduction with Shiga Toxin 1-Encoding Phage
1998 ◽
Vol 66
(9)
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pp. 4496-4498
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Keyword(s):
E Coli
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ABSTRACT To facilitate the study of intestinal transmission of the Shiga toxin 1 (Stx1)-converting phage H-19B, Tn10d-blamutagenesis of an Escherichia coli H-19B lysogen was undertaken. Two mutants containing insertions in the gene encoding the A subunit of Stx1 were isolated. The resultant ampicillin-resistantE. coli strains lysogenic for these phages produced infectious H-19B particles but not active toxin. These lysogens were capable of transducing an E. coli recipient strain in the murine gastrointestinal tract, thereby demonstrating that lysogens of Shiga toxin-converting phages give rise to infectious virions within the host gastrointestinal tract.
Keyword(s):
2007 ◽
Vol 73
(10)
◽
pp. 3144-3150
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Keyword(s):