scholarly journals Natural Immunity to Ascaris lumbricoidesAssociated with Immunoglobulin E Antibody to ABA-1 Allergen and Inflammation Indicators in Children

1999 ◽  
Vol 67 (2) ◽  
pp. 484-489 ◽  
Author(s):  
Charles McSharry ◽  
Yu Xia ◽  
Celia V. Holland ◽  
Malcolm W. Kennedy
Keyword(s):  
Immunoglobulin E ◽  
High Titer ◽  
Eosinophil Cationic Protein ◽  
Antibody Activity ◽  
Natural Immunity ◽  
Igg Antibody ◽  
Cationic Protein ◽  
Protective Function ◽  
Ige Antibody ◽  
Inflammatory Processes

ABSTRACT Children putatively immune to the large roundworm Ascaris lumbricoides were identified in an area of Nigeria where infection is hyperendemic. Immunity was associated with higher levels of serum ferritin, C-reactive protein, and eosinophil cationic protein, indicating ongoing acute phase or inflammatory processes. In contrast, children who were susceptible to the infection had little serological evidence of inflammation despite their high parasite burdens. Immunoglobulin G (IgG) antibody activity in all subclasses was present in high titer in most children but appeared to have no protective function. Despite exceptionally high total IgE levels, there was no evidence that atopic responses to local common allergens was associated with natural immunity to Ascaris. Among those individuals who produced IgG antibody to recombinant ABA-1 allergen ofAscaris, the naturally immune group had significantly more IgE antibody to the allergen than did those susceptible to the infection. IgE antibody responses in conjunction with innate inflammatory processes therefore appear to associate with natural immunity to ascariasis.

scholarly journals Traditional Chinese MedicineZHENGIdentification Provides a Novel Stratification Approach in Patients with Allergic Rhinitis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Kai-Li Liang ◽  
Rong-San Jiang ◽  
Chia-Lin Lee ◽  
Pei-Jung Chiang ◽  
Jui-Shan Lin ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Immunoglobulin E ◽  
Linear Regression Analysis ◽  
Eosinophil Cationic Protein ◽  
Multiple Linear Regression Analysis ◽  
Total Serum ◽  
Blood Eosinophil ◽  
Cationic Protein ◽  
Explanatory Factors ◽  
Severity Scores

Background. We aimed to apply theZHENGidentification to provide an easy and useful tool to stratify the patients with allergic rhinitis (AR) through exploring the correlation between the quantified scores of AR symptoms and the TCMZHENGs.Methods. A total of 114 AR patients were enrolled in this observational study. All participants received the examinations of anterior rhinoscopy and acoustic rhinometry. Their blood samples were collected for measurement of total serum immunoglobulin E (IgE), blood eosinophil count (Eos), and serum eosinophil cationic protein (ECP). They also received two questionnaire to assess the severity scores of AR symptoms and quantified TCMZHENGscores. Multiple linear regression analysis was used to determine explanatory factors for the score of AR manifestations.Results. IgE and ECP level, duration of AR, the 2 derived TCMZHENGscores of “Yin-Xu − Yang-Xu”, and “Qi-Xu+Blood-Xu” were 5 explanatory variables to predict the severity scores of AR symptoms. The patients who had higher scores of “Yin-Xu − Yang-Xu” or “Qi-Xu+Blood-Xu” tended to manifest as “sneezer and runner” or “blockers,” respectively.Conclusions.The TCMZHENGscores correlated with the severity scores of AR symptoms and provided an easy and useful tool to stratify the AR patients.

Allergy tests

2013 ◽  
pp. 533-554
Author(s):  
Gavin P Spickett
Keyword(s):  
Drug Allergy ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Ige Receptor ◽  
Cationic Protein ◽  
Challenge Tests ◽  
Specific Igg ◽  
Receptor Antibodies

Introduction Allergen-specific IgE Allergen-specific IgG antibodies Basophil activation test CD23, soluble (Fcε‎ receptor) C3a, C4a, and C5a (anaphylotoxins) Challenge tests Drug allergy testing Eosinophil cationic protein (ECP) Eosinophil count Flow-CAST® and CAST-ELISA® Histamine-release assays Immunoglobulin E (total IgE) IgE autoantibodies/IgE receptor antibodies...

scholarly journals Faecal eosinophil cationic protein and serum immunoglobulin E in relation to infant feeding practices

2016 ◽  
Vol 54 (2) ◽  
pp. 246-252 ◽  
Author(s):  
Man-Chin Hua ◽  
Chien-Chang Chen ◽  
Sui-Ling Liao ◽  
Tsung-Chieh Yao ◽  
Ming-Han Tsai ◽  
...  
Keyword(s):  
Intestinal Inflammation ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Serum Immunoglobulin ◽  
Breastfeeding Duration ◽  
Total Serum ◽  
Cationic Protein ◽  
Solid Foods ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

Background To date, the effects of exclusive breastfeeding duration and timing of solid food introduction on allergy prevention are unclear. The aim of this study was to determine the effect of variable feeding practices on intestinal inflammation in infants using faecal eosinophil cationic protein as a surrogate marker and to assess whether faecal eosinophil cationic protein is associated with serum immunoglobulin E. Methods Subjects ( n = 206) were enrolled from the Prediction of Allergies in Taiwanese CHildren (PATCH) birth cohort study. Stool samples were collected at 6 and 12 months for determining eosinophil cationic protein, and blood was collected for determining total and allergen-specific immunoglobulin E at 12 months. We compared these biomarkers between infants with variable exclusive breastfeeding duration and infants introduced to solid foods at various periods. The association between faecal eosinophil cationic protein, total serum immunoglobulin E and specific immunoglobulin E was also analysed. Results Faecal eosinophil cationic protein was significantly higher in exclusively breastfed infants compared with formula-fed infants and infants who were not exclusively breastfed at 6 months of age ( P < 0.05). At 12 months, infants who were introduced to solid foods at 5–6 months had the lowest faecal eosinophil cationic protein compared with those who were introduced at earlier and later periods. There was no significant association between faecal eosinophil cationic protein and serum immunoglobulin E. Conclusion We found that breastfeeding exclusively for >6 months did not reduce serum immunoglobulin E, but rather increased intestinal inflammation. Faecal eosinophil cationic protein was not associated with total serum immunoglobulin E and specific immunoglobulin E and might not be a useful indictor of immunoglobulin E sensitization in infancy.

scholarly journals Non-allergic activation of eosinophils after strenuous endurance exercise

2004 ◽  
Vol 16 (2) ◽  
pp. 12
Author(s):  
AJ McKune ◽  
LL Smith ◽  
SJ Semple ◽  
AA Wadee
Keyword(s):  
Endurance Exercise ◽  
Sports Medicine ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Upper Respiratory Tract ◽  
Serum Concentrations ◽  
Cationic Protein ◽  
Level Of Significance

Objective. To determine the effect of prolonged endurance exercise on the serum concentrations of eosinophil cationic protein (ECP), immunoglobulin E (IgE) and upper respiratory tract symptoms (URTS). Design. In 11 healthy, experienced volunteers (6 males, 5 females, age 43 ± 9.8 years) the serum concentrations of ECP and IgE were measured, 24 hours prior to projected finishing time, immediately post exercise (IPE), and 3 h, 24 h, and 72 h after an ultramarathon (90 km). Self-reported URTS were also recorded for 14 days after the race. ECP was measured using radioimmunoassay and IgE using the Alastat Microplate Total IgE kit. The after-exercise values were corrected for plasma volume changes, which were calculated from haematocrit and haemoglobin values. Serum concentrations of ECP and IgE were analysed using an analysis of variance (ANOVA) comparing values with before-exercise levels. Level of significance was set at p ≤ 0.05. Results. ECP was significantly elevated at 72 hours (+52%), whilst IgE was not significantly altered after the ultramarathon. There were no reported URTS for the 14 days after the race. Conclusion. The eosinophil is a pro-inflammatory leukocyte involved in bronchial hyperreactivity and allergic inflammation of the airways. IgE is associated with allergic diseases such as asthma and rhinitis. Serum ECP is a sensitive marker of eosinophil activation. The result provides evidence for the non-allergic activation of blood eosinophils during prolonged endurance exercise. Whether this indicates exercise or environmentally induced airway inflammation, or a role for ECP in muscle /tissue repair, are hypotheses that require additional research. SA Sports Medicine Vol.16(2) 2004: 12-16

scholarly journals Endotype of Eosinophilic Nasal Polyposis According to the Presence of Atopy

2020 ◽  
Vol 63 (12) ◽  
pp. 579-585
Author(s):  
Do Hyun Kim ◽  
Boo-Young Kim ◽  
Il Hwan Lee ◽  
Sung Won Kim ◽  
Soo Whan Kim
Keyword(s):  
Nasal Polyps ◽  
Nasal Polyposis ◽  
Transforming Growth Factor ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Transforming Growth Factor Β ◽  
Orphan Receptor ◽  
Cationic Protein ◽  
Group A ◽  
Eosinophil Counts

Background and Objectives We evaluated differences in the pathophysiology of atopic and non-atopic eosinophilic nasal polyps and investigated their distinct inflammatory profiles.Subjects and Method A total of 36 patients were recruited: 10 controls (Group C), 14 with chronic rhinosinusitis with eosinophilic nasal polyps with atopy (Group A), and 12 without atopy (Group NA).Results Serum eosinophil counts, total immunoglobulin E, eosinophil cationic protein levels, and tissue eosinophil counts were elevated in Groups A and NA vs. Group C. Real-time polymerase chain reaction results showed increased GATA-3, interleukin (IL)-4, IL-33 levels, but decreased levels of retinoic acid-related orphan receptor gamma t, IL-17 in Groups A and NA. Related to the regulatory T (T-reg) cell response, forkhead box P3 (Foxp3<sup>+</sup>) (A: <i>p</i><0.001, NA: <i>p</i><0.001) and IL-10 (A: <i>p</i><0.001, NA: <i>p</i><0.001) levels were elevated and transforming growth factor-β levels (A: <i>p</i><0.001, NA: <i>p</i><0.001) were decreased in Group A and Group NA in comparison to those in Group C. The Foxp3<sup>+</sup> (<i>p</i>=0.001) and IL-10 (<i>p</i><0.001) were significantly higher in Group A than in Group NA.Conclusion T-reg cells and IL-10 may be major factors differentiating the pathophysiology of atopic and non-atopic eosinophilic nasal polyps, and the T helper (Th) 2/Th17/T-reg imbalance might be important in the development of eosinophilic nasal polyposis.

Correlation of serum interleukin-31 with pruritus and blood eosinophil markers in children with atopic dermatitis

2020 ◽  
Vol 41 (1) ◽  
pp. 59-65
Author(s):  
Jung Hye Byeon ◽  
Wonsuck Yoon ◽  
So Hyun Ahn ◽  
Hyo Sun Lee ◽  
Seunghyun Kim ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Skin Inflammation ◽  
Clinical Severity ◽  
Blood Eosinophil ◽  
Healthy Children ◽  
Cationic Protein ◽  
Clinical Biomarkers ◽  
Significant Difference

Background: Atopic dermatitis (AD) is chronic pruritic inflammatory skin disease in children. Interleukin (IL) 31 is a recently discovered cytokine associated with chronic skin inflammation and pruritus. Objectives: The aims of this study were to determine whether serum IL-31 levels are increased in children with AD and to examine the relationship between IL-31 and other clinical biomarkers in AD. Methods: Serum cytokine levels, including IL-31, IL-4, and IL-12, were measured in 38 patients with AD and 10 healthy children. Peripheral blood eosinophils, serum immunoglobulin E levels, eosinophil cationic protein, and thymic stromal lymphopoietin (TSLP) were measured. We also estimated the clinical severity of AD by using the Scoring Atopic Dermatitis (SCORAD) index by a single clinician. Results: The serum IL-31 levels were significantly higher in the patients with AD than in the healthy children. IL-31 correlated well with the SCORAD index and blood eosinophilic inflammatory markers. The serum level of TSLP was also higher in patients with AD than in the healthy children; however, levels of IL-4 and IL-12 were not different between AD and healthy children. There was no significant difference in serum IL-31 levels between patients with atopic AD and nonatopic AD. Conclusion: This study showed that serum IL-31 levels were significantly elevated in patients with AD than in the healthy children and correlated well with disease severity. IL-31 seemed to be one of the cytokines that induce pruritus and eosinophilic inflammation in AD. Serum IL-31 correlated with pruritic symptoms and disease course of AD.

scholarly journals Non-allergic activation of eosinophils after strenuous endurance exercise

2004 ◽  
Vol 16 (2) ◽  
pp. 12 ◽  
Author(s):  
AJ McKune ◽  
LL Smith ◽  
SJ Semple ◽  
AA Wadee
Keyword(s):  
Endurance Exercise ◽  
Sports Medicine ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Upper Respiratory Tract ◽  
Serum Concentrations ◽  
Cationic Protein ◽  
Level Of Significance

Objective. To determine the effect of prolonged endurance exercise on the serum concentrations of eosinophil cationic protein (ECP), immunoglobulin E (IgE) and upper respiratory tract symptoms (URTS). Design. In 11 healthy, experienced volunteers (6 males, 5 females, age 43 ± 9.8 years) the serum concentrations of ECP and IgE were measured, 24 hours prior to projected finishing time, immediately post exercise (IPE), and 3 h, 24 h, and 72 h after an ultramarathon (90 km). Self-reported URTS were also recorded for 14 days after the race. ECP was measured using radioimmunoassay and IgE using the Alastat Microplate Total IgE kit. The after-exercise values were corrected for plasma volume changes, which were calculated from haematocrit and haemoglobin values. Serum concentrations of ECP and IgE were analysed using an analysis of variance (ANOVA) comparing values with before-exercise levels. Level of significance was set at p ≤ 0.05. Results. ECP was significantly elevated at 72 hours (+52%), whilst IgE was not significantly altered after the ultramarathon. There were no reported URTS for the 14 days after the race. Conclusion. The eosinophil is a pro-inflammatory leukocyte involved in bronchial hyperreactivity and allergic inflammation of the airways. IgE is associated with allergic diseases such as asthma and rhinitis. Serum ECP is a sensitive marker of eosinophil activation. The result provides evidence for the non-allergic activation of blood eosinophils during prolonged endurance exercise. Whether this indicates exercise or environmentally induced airway inflammation, or a role for ECP in muscle /tissue repair, are hypotheses that require additional research. SA Sports Medicine Vol.16(2) 2004: 12-16

Immune responses in children infected with the pinwormEnterobius vermicularisin central Greece

2015 ◽  
Vol 90 (3) ◽  
pp. 337-341 ◽  
Author(s):  
G.G. Patsantara ◽  
E.-T. Piperaki ◽  
C. Tzoumaka-Bakoula ◽  
M.G. Kanariou
Keyword(s):  
Immune Response ◽  
Allergic Disease ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Protective Role ◽  
Intestinal Helminth ◽  
Cationic Protein ◽  
Central Greece ◽  
History Of

AbstractPrevious studies have suggested an immunomodulatory and even protective role forEnterobius vermicularis, the least pathogenic human intestinal helminth. Here, in a study using haematological and serological parameters, we tested a total of 215 children from central Greece, with a mean age of 8.39, of whom 105 (48.84%) were infected withE. vermicularisand 110 (51.16%) were matched healthy controls. In particular, we analysed eosinophil counts (EO), serum eosinophil cationic protein (ECP), total and specific serum immunoglobulin E (IgE) and the ECP/EO ratio. The atopic status and the potential occurrence of clinically expressed allergic diseases were both taken into account. Eosinophils, ECP and IgE were found to be higher in infected than in uninfected children, indicating a type-2 immune response activation during infection. Atopic infected children exhibited higher IgE levels compared to non-atopic ones. EO and ECP were found to be lower in atopic children who had a history of allergic disease than in those with no such history. The type-2 oriented immune response elicited againstE. vermiculariscould contribute to a balanced activation of the immune system in the examined children. Interestingly, although the atopic children showed a stronger activation, they did not exhibit any symptoms and, moreover, there seemed to be some indication of immunosuppression in those children with a positive history of allergic disease.

High Eosinophil Cationic Protein Level in Asthmatic Patients with "Heat" Zheng

2003 ◽  
Vol 31 (02) ◽  
pp. 277-283 ◽  
Author(s):  
Ching-Hsaing Hsu ◽  
Min-Chien Yu ◽  
Chang-Hsien Lee ◽  
Tsung-Cheng Lee ◽  
Su-Yu Yang
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Serum Level ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Allergic Reactions ◽  
Cationic Protein ◽  
Asthmatic Patients ◽  
Yin And Yang ◽  
Total Immunoglobulin

In traditional Chinese medicine (TCM), the imbalance of yin and yang is one of the basic pathogeneses of a disease. Preponderance of yang leads to "heat" manifestations including thirst, dryness of the throat, dark scanty urine and constipation. Treatment of asthma in TCM is based on the differentiation of "heat" Zheng according to the manifestations. Some of the patients with allergic asthma also present typical "heat" manifestations. To investigate the essence of "heat" manifestation in asthma, we measured the serum level of eosinophil cationic protein (ECP) in asthmatic patients. ECP usually represents the activation of eosinophils which are the main effectors in late allergic reactions. Our results demonstrated that asthmatic patients with "heat" manifestations had higher serum ECP levels, compared to those without "heat" manifestations (34.3 ± 4 μg/l versus 15.3 ± 3 μg/l). However, total immunoglobulin E (IgE), and the eosinophil count in peripheral blood did not show any difference between the "heat" and "non-heat" groups. Therefore, we conclude that ECP in asthmatic patients plays an important role in the development of "heat" manifestations as diagnosed by TCM.

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