Allergy tests

2013 ◽  
pp. 533-554
Author(s):  
Gavin P Spickett
Keyword(s):  
Drug Allergy ◽  
Immunoglobulin E ◽  
Eosinophil Cationic Protein ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Ige Receptor ◽  
Cationic Protein ◽  
Challenge Tests ◽  
Specific Igg ◽  
Receptor Antibodies

Introduction Allergen-specific IgE Allergen-specific IgG antibodies Basophil activation test CD23, soluble (Fcε‎ receptor) C3a, C4a, and C5a (anaphylotoxins) Challenge tests Drug allergy testing Eosinophil cationic protein (ECP) Eosinophil count Flow-CAST® and CAST-ELISA® Histamine-release assays Immunoglobulin E (total IgE) IgE autoantibodies/IgE receptor antibodies...

A Pentaherbs Capsule as a Treatment Option for Atopic Dermatitis in Children: An Open-Labeled Case Series

2004 ◽  
Vol 32 (06) ◽  
pp. 941-950 ◽  
Author(s):  
Kam-Lun Ellis Hon ◽  
Ting-Fan Leung ◽  
Yin Wong ◽  
Wai-Kei Christopher Lam ◽  
De-Qi Bill Guan ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Immunoglobulin E ◽  
Complete Blood Count ◽  
Eosinophil Cationic Protein ◽  
Case Series ◽  
Specific Ige ◽  
Controlled Study ◽  
Therapeutic Effects ◽  
Cationic Protein ◽  
Beneficial Effects

Traditional Chinese Medicine (TCM) has been used in patients with atopic dermatitis (AD), but its therapeutic effects are debatable. We evaluated the clinical and biochemical effects of a TCM capsule (PentaHerbs capsule) in children with AD. After a run-in period of 4 weeks, children old enough to manage oral medication were admitted and their disease severity was evaluated by the SCORing Atopic Dermatitis (SCORAD) index. Blood was obtained for complete blood count, total and allergen-specific immunoglobulin E (IgE), biochemical studies and inflammatory markers of AD severity [serum cutaneous T cell-attracting chemokine (CTACK), macrophage-derived chemokine (MDC), thymus and activation-regulated chemokine (TARC) and eosinophil cationic protein (ECP)] prior to, and after 3 months of, TCM use. Three PentaHerbs capsules twice a day were prescribed for 4 months. Patients were followed monthly to ensure compliance, and SCORAD scores were obtained at each visit. Five boys and four girls participated in the study. All patients had detectable food or inhalant-specific IgE in serum. There was significant improvement in the overall and component SCORAD scores. There were no significant differences between the pre- and post-treatment values of the serum CTACK, MDC, TARC and ECP levels but CTACK showed a decreasing trend (p=0.069). No clinical or biochemical evidence of any adverse drug reaction was observed during the study period. The PentaHerbs capsules were well tolerated by the children and apparent beneficial effects were noted clinically. A larger, randomized placebo-controlled study is required to confirm the efficacy of this formulation for AD.

scholarly journals Neo-antigens for the serological diagnosis of IgE-mediated drug allergic reactions to antibiotics cephalosporin, carbapenem and monobactam

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Edurne Peña-Mendizabal ◽  
Sergi Morais ◽  
Ángel Maquieira
Keyword(s):  
Drug Allergy ◽  
High Sensitivity ◽  
Specific Ige ◽  
In Vitro Tests ◽  
Allergic Reactions ◽  
Serum Samples ◽  
Lactam Ring ◽  
Specific Igg ◽  
Ige Mediated

Abstract New antigens deriving from -lloyl and -llanyl, major and minor determinants, respectively, were produced for β-lactam antibiotics cefuroxime, cefotaxime, ceftriaxone, meropenem and aztreonam. Twenty β-lactam antigens were produced using human serum albumin and histone H1 as carrier proteins. Antigens were tested by multiplex in vitro immunoassays and evaluated based on the detection of specific IgG and IgE in the serum samples. Both major and minor determinants were appropriate antigens for detecting specific anti-β-lactam IgG in immunised rabbit sera. In a cohort of 37 allergic patients, we observed that only the minor determinants (-llanyl antigens) were suitable for determining specific anti-β-lactam IgE antibodies with high sensitivity (< 0.01 IU/mL; 24 ng/L) and specificity (100%). These findings reveal that not only the haptenisation of β-lactam antibiotics renders improved molecular recognition events when the 4-member β-lactam ring remains unmodified, but also may contribute to develop promising minor antigens suitable for detecting specific IgE-mediated allergic reactions. This will facilitate the development of sensitive and selective multiplexed in vitro tests for drug-allergy diagnoses to antibiotics cephalosporin, carbapenem and monobactam.

scholarly journals Human toxocariasis and atopy

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 32 ◽  
Author(s):  
Jean-François Magnaval ◽  
Judith Fillaux ◽  
Sophie Cassaing ◽  
Alexis Valentin ◽  
Xavier Iriart ◽  
...  
Keyword(s):  
Eosinophil Cationic Protein ◽  
Multivariate Regression Analysis ◽  
Specific Ige ◽  
Outcome Variable ◽  
Bivariate Analysis ◽  
Blood Eosinophil ◽  
University Hospitals ◽  
Cationic Protein ◽  
Serum Total Ige ◽  
Inhalant Allergens

To assess the possible influence of atopy on the clinical picture of human toxocariasis, a retrospective study was carried out using file records for patients who attended the Outpatient Clinic of Parasitology in Toulouse University Hospitals. A total of 106 file records for patients who had been diagnosed with common/covert toxocariasis were extracted from the database. Forty-nine patients (20 females and 29 males) were considered atopic since they exhibited a long (≥ 1 year) history of various allergic issues along with a titer ≥ 0.7 kIU/L for specific IgE against at least two out of nine mixes of common inhalant allergens. Fifty-seven patients (42 females and 15 males) were designated nonatopic on the basis of a negative result (<0.35 kIU/L) of the test for specific IgE. Demographic (age and sex), clinical (20 signs or symptoms) and laboratory (blood eosinophil count, eosinophil cationic protein, serum total IgE, and specific anti-Toxocara IgE) variables were investigated by bivariate analysis followed by multivariate regression analysis using “atopy” as the outcome variable. On the basis of our results, the clinical or laboratory picture of toxocaral disease was not affected by the presence of an atopic status.

Eosinophil cationic protein, specific IgE and IgG4 in human toxocariasis

2006 ◽  
Vol 80 (4) ◽  
pp. 417-423 ◽  
Author(s):  
J.-F. Magnaval ◽  
J.-H. Faufingue ◽  
B. Morassin ◽  
R. Fabre
Keyword(s):  
Eosinophil Cationic Protein ◽  
Specific Ige ◽  
Blood Eosinophil ◽  
Regression Analyses ◽  
Cationic Protein ◽  
Blood Eosinophil Count ◽  
Atopic Status ◽  
Serum Total Ige ◽  
Inhalant Allergens ◽  
High Level

AbstractAmong 67 French patients presenting a toxocaral infection, various demographic, environmental, clinical and laboratory parameters (blood eosinophil count, eosinophil cationic protein (ECP), serum total IgE, specific IgE against common inhalant allergens, specific IgE and IgG4 againstToxocaraexcretory-secretory antigens) were investigated. Correlation studies and logistic regression analyses were conducted, testing elevated levels of ECP, specific anti-ToxocaraIgE or IgG4 as outcome variables An elevated ECP level was significantly associated with both cough and rhinitis, a high level of specific anti-ToxocaraIgE with itchy rashes and possible atopic status, and an increase of specific anti-ToxocaraIgG4 with rural residence.

scholarly journals Traditional Chinese MedicineZHENGIdentification Provides a Novel Stratification Approach in Patients with Allergic Rhinitis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Kai-Li Liang ◽  
Rong-San Jiang ◽  
Chia-Lin Lee ◽  
Pei-Jung Chiang ◽  
Jui-Shan Lin ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Immunoglobulin E ◽  
Linear Regression Analysis ◽  
Eosinophil Cationic Protein ◽  
Multiple Linear Regression Analysis ◽  
Total Serum ◽  
Blood Eosinophil ◽  
Cationic Protein ◽  
Explanatory Factors ◽  
Severity Scores

Background. We aimed to apply theZHENGidentification to provide an easy and useful tool to stratify the patients with allergic rhinitis (AR) through exploring the correlation between the quantified scores of AR symptoms and the TCMZHENGs.Methods. A total of 114 AR patients were enrolled in this observational study. All participants received the examinations of anterior rhinoscopy and acoustic rhinometry. Their blood samples were collected for measurement of total serum immunoglobulin E (IgE), blood eosinophil count (Eos), and serum eosinophil cationic protein (ECP). They also received two questionnaire to assess the severity scores of AR symptoms and quantified TCMZHENGscores. Multiple linear regression analysis was used to determine explanatory factors for the score of AR manifestations.Results. IgE and ECP level, duration of AR, the 2 derived TCMZHENGscores of “Yin-Xu − Yang-Xu”, and “Qi-Xu+Blood-Xu” were 5 explanatory variables to predict the severity scores of AR symptoms. The patients who had higher scores of “Yin-Xu − Yang-Xu” or “Qi-Xu+Blood-Xu” tended to manifest as “sneezer and runner” or “blockers,” respectively.Conclusions.The TCMZHENGscores correlated with the severity scores of AR symptoms and provided an easy and useful tool to stratify the AR patients.

scholarly journals Natural Immunity to Ascaris lumbricoidesAssociated with Immunoglobulin E Antibody to ABA-1 Allergen and Inflammation Indicators in Children

1999 ◽  
Vol 67 (2) ◽  
pp. 484-489 ◽  
Author(s):  
Charles McSharry ◽  
Yu Xia ◽  
Celia V. Holland ◽  
Malcolm W. Kennedy
Keyword(s):  
Immunoglobulin E ◽  
High Titer ◽  
Eosinophil Cationic Protein ◽  
Antibody Activity ◽  
Natural Immunity ◽  
Igg Antibody ◽  
Cationic Protein ◽  
Protective Function ◽  
Ige Antibody ◽  
Inflammatory Processes

ABSTRACT Children putatively immune to the large roundworm Ascaris lumbricoides were identified in an area of Nigeria where infection is hyperendemic. Immunity was associated with higher levels of serum ferritin, C-reactive protein, and eosinophil cationic protein, indicating ongoing acute phase or inflammatory processes. In contrast, children who were susceptible to the infection had little serological evidence of inflammation despite their high parasite burdens. Immunoglobulin G (IgG) antibody activity in all subclasses was present in high titer in most children but appeared to have no protective function. Despite exceptionally high total IgE levels, there was no evidence that atopic responses to local common allergens was associated with natural immunity to Ascaris. Among those individuals who produced IgG antibody to recombinant ABA-1 allergen ofAscaris, the naturally immune group had significantly more IgE antibody to the allergen than did those susceptible to the infection. IgE antibody responses in conjunction with innate inflammatory processes therefore appear to associate with natural immunity to ascariasis.

THE HIGH-AFFINITY IMMUNOGLOBULIN E (IgE) RECEPTOR (FcεRI) MEDIATES IgE-DEPENDENT ALLERGEN PRESENTATION

PEDIATRICS ◽  
1996 ◽  
Vol 98 (2) ◽  
pp. 325-325
Author(s):  
Russell Hopp
Keyword(s):  
Immunoglobulin E ◽  
Late Phase ◽  
Specific Ige ◽  
Delayed Response ◽  
Target Area ◽  
Type I ◽  
Ige Receptor ◽  
High Affinity ◽  
Ige Receptors ◽  
Response Target

Low numbers of monocytes with highaffinity IgE receptors and bound-specific IgE, attracted to the late-phase response target area (skin, nose, or lungs) may be a prime cell for the initiation of further Type I immediate and Type I late-phase responses (referred to as T cell mediated, delayed response, in this paper).

Specific IgE antibodies and serum eosinophil cationic protein in children with atopic dermatitis alone

1994 ◽  
Vol 36 (2) ◽  
pp. 146-149 ◽  
Author(s):  
ATSUO URISU ◽  
YASUTO KONDO ◽  
EIKO WADA ◽  
FUMIYA HORIBA ◽  
MITSUTOSI TSURUTA ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Eosinophil Cationic Protein ◽  
Specific Ige ◽  
Cationic Protein ◽  
Ige Antibodies ◽  
Specific Ige Antibodies
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