The Cloned Locus of Enterocyte Effacement from EnterohemorrhagicEscherichia coli O157:H7 Is Unable To Confer the Attaching and Effacing Phenotype upon E. coliK-12
1999 ◽
Vol 67
(8)
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pp. 4260-4263
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Keyword(s):
E Coli
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ABSTRACT The locus of enterocyte effacement (LEE) pathogenicity island of enterohemorrhagic Escherichia coli (EHEC) O157:H7 possesses the same genes in identical order and orientation as the LEE of enteropathogenic E. coli (EPEC) O127:H6 but is unable to form attaching and effacing (A/E) lesions or to secrete Esp proteins when it is cloned in an E. coli K-12 background. The A/E phenotype could not be restored by trans complementation with a variety of cloned EPEC LEE fragments, suggesting functional and/or regulatory differences between the LEE pathogenicity islands of EPEC O127:H6 and EHEC O157:H7.
1998 ◽
Vol 66
(6)
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pp. 2553-2561
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1998 ◽
Vol 66
(8)
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pp. 3810-3817
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