scholarly journals Phosphate-Enhanced Stationary-Phase Fitness of Escherichia coli Is Related to Inorganic Polyphosphate Level

2009 ◽  
Vol 191 (13) ◽  
pp. 4478-4481 ◽  
Author(s):  
Lici A. Schurig-Briccio ◽  
Ricardo N. Farías ◽  
María R. Rintoul ◽  
Viviana A. Rapisarda
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Stationary Phase ◽  
Enzyme Activities ◽  
Late Stationary Phase ◽  
Inorganic Polyphosphate

ABSTRACT We found that Escherichia coli grown in media with >37 mM phosphate maintained a high polyphosphate level in late stationary phase, which could account for changes in gene expression and enzyme activities that enhance stationary-phase fitness.

SlyD-dependent nickel delivery limits maturation of [NiFe]-hydrogenases in late-stationary phase Escherichia coli cells

Metallomics ◽  
2015 ◽  
Vol 7 (4) ◽  
pp. 683-690 ◽  
Author(s):  
Constanze Pinske ◽  
Frank Sargent ◽  
R. Gary Sawers
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Late Stationary Phase ◽  
E Coli ◽  
Escherichia Coli Cells

The metallochaperone SlyD is essential for nickel delivery to hydrogenase in stationary phaseE. colicells.

scholarly journals Identical, Independent, and Opposing Roles of ppGpp and DksA in Escherichia coli

2007 ◽  
Vol 189 (14) ◽  
pp. 5193-5202 ◽  
Author(s):  
Lisa U. Magnusson ◽  
Bertil Gummesson ◽  
Predrag Joksimović ◽  
Anne Farewell ◽  
Thomas Nyström
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Amino Acid ◽  
Stationary Phase ◽  
Cell Aggregation ◽  
Phase Morphology ◽  
Deficient Mutant ◽  
Similarities And Differences ◽  
Opposing Effects ◽  
Cell Cell

ABSTRACT The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effects on many of the observed phenotypes but that DksA and ppGpp also have independent and sometimes opposing roles in the cell. Specifically, we show that overexpression of DksA can compensate for the loss of ppGpp with respect to transcription of the promoters P uspA , P livJ , and P rrnBP1 as well as amino acid auxotrophy, cell-cell aggregation, motility, filamentation, and stationary phase morphology, suggesting that DksA can function without ppGpp in regulating gene expression. In addition, ppGpp and DksA have opposing effects on adhesion. In the course of our analysis, we also discovered new features of the relaxed mutant, namely, defects in cell-cell aggregation and motility.

scholarly journals Complex Physiology and Compound Stress Responses during Fermentation of Alkali-Pretreated Corn Stover Hydrolysate by an Escherichia coli Ethanologen

2012 ◽  
Vol 78 (9) ◽  
pp. 3442-3457 ◽  
Author(s):  
Michael S. Schwalbach ◽  
David H. Keating ◽  
Mary Tremaine ◽  
Wesley D. Marner ◽  
Yaoping Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Amino Acids ◽  
Stationary Phase ◽  
Gene Expression Profiling ◽  
Stress Responses ◽  
Expression Profiling ◽  
Content Type ◽  
E Coli ◽  
Cell Maintenance

ABSTRACTThe physiology of ethanologenicEscherichia coligrown anaerobically in alkali-pretreated plant hydrolysates is complex and not well studied. To gain insight into howE. coliresponds to such hydrolysates, we studied anE. coliK-12 ethanologen fermenting a hydrolysate prepared from corn stover pretreated by ammonia fiber expansion. Despite the high sugar content (∼6% glucose, 3% xylose) and relatively low toxicity of this hydrolysate,E. coliceased growth long before glucose was depleted. Nevertheless, the cells remained metabolically active and continued conversion of glucose to ethanol until all glucose was consumed. Gene expression profiling revealed complex and changing patterns of metabolic physiology and cellular stress responses during an exponential growth phase, a transition phase, and the glycolytically active stationary phase. During the exponential and transition phases, high cell maintenance and stress response costs were mitigated, in part, by free amino acids available in the hydrolysate. However, after the majority of amino acids were depleted, the cells entered stationary phase, and ATP derived from glucose fermentation was consumed entirely by the demands of cell maintenance in the hydrolysate. Comparative gene expression profiling and metabolic modeling of the ethanologen suggested that the high energetic cost of mitigating osmotic, lignotoxin, and ethanol stress collectively limits growth, sugar utilization rates, and ethanol yields in alkali-pretreated lignocellulosic hydrolysates.

scholarly journals Global Role for ClpP-Containing Proteases in Stationary-Phase Adaptation of Escherichia coli

2003 ◽  
Vol 185 (1) ◽  
pp. 115-125 ◽  
Author(s):  
Dieter Weichart ◽  
Nadine Querfurth ◽  
Mathias Dreger ◽  
Regine Hengge-Aronis
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Growth Phase ◽  
Proteome Analysis ◽  
Resting Cells ◽  
Wild Type ◽  
Late Stationary Phase ◽  
E Coli ◽  
In The Wild ◽  
Dps Protein

ABSTRACT To elucidate the involvement of proteolysis in the regulation of stationary-phase adaptation, the clpA, clpX, and clpP protease mutants of Escherichia coli were subjected to proteome analysis during growth and during carbon starvation. For most of the growth-phase-regulated proteins detected on our gels, the clpA, clpX, or clpP mutant failed to mount the growth-phase regulation found in the wild type. For example, in the clpP and clpA mutant cultures, the Dps protein, the WrbA protein, and the periplasmic lysine-arginine-ornithine binding protein ArgT did not display the induction typical for late-stationary-phase wild-type cells. On the other hand, in the protease mutants, a number of proteins accumulated to a higher degree than in the wild type, especially in late stationary phase. The proteins affected in this manner include the LeuA, TrxB, GdhA, GlnA, and MetK proteins and alkyl hydroperoxide reductase (AhpC). These proteins may be directly degraded by ClpAP or ClpXP, respectively, or their expression could be modulated by a protease-dependent mechanism. From our data we conclude that the levels of most major growth-phase-regulated proteins in E. coli are at some point controlled by the activity of at least one of the ClpP, ClpA, and ClpX proteins. Cultures of the strains lacking functional ClpP or ClpX also displayed a more rapid loss of viability during extended stationary phase than the wild type. Therefore, regulation by proteolysis seems to be more important, especially in resting cells, than previously suspected.

scholarly journals RpoS-dependent regulation of genes expressed at late stationary phase in Escherichia coli

FEBS Letters ◽  
1996 ◽  
Vol 386 (2-3) ◽  
pp. 177-180 ◽  
Author(s):  
Ali Azam Talukder ◽  
Satoshi Yanai ◽  
Takeshi Nitta ◽  
Akio Kato ◽  
Mamoru Yamada
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Late Stationary Phase

scholarly journals Escherichia coli produces linoleic acid during late stationary phase.

1993 ◽  
Vol 175 (17) ◽  
pp. 5324-5328 ◽  
Author(s):  
H D Rabinowitch ◽  
D Sklan ◽  
D H Chace ◽  
R D Stevens ◽  
I Fridovich
Keyword(s):  
Escherichia Coli ◽  
Linoleic Acid ◽  
Stationary Phase ◽  
Late Stationary Phase

scholarly journals Stationary-Phase Quorum-Sensing Signals Affect Autoinducer-2 and Gene Expression in Escherichia coli

2004 ◽  
Vol 70 (4) ◽  
pp. 2038-2043 ◽  
Author(s):  
Dacheng Ren ◽  
Laura A. Bedzyk ◽  
Rick W. Ye ◽  
Stuart M. Thomas ◽  
Thomas K. Wood
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Quorum Sensing ◽  
Stationary Phase ◽  
Growth Stages ◽  
E Coli ◽  
Stationary Culture ◽  
Autoinducer 2 ◽  
K 12 ◽  
Quorum Sensing Signals

ABSTRACT Quorum sensing via autoinducer-2 (AI-2) has been identified in different strains, including those from Escherichia, Vibrio, Streptococcus, and Bacillus species, and previous studies have suggested the existence of additional quorum-sensing signals working in the stationary phase of Escherichia coli cultures. To investigate the presence and global effect of these possible quorum-sensing signals other than AI-2, DNA microarrays were used to study the effect of stationary-phase signals on the gene expression of early exponential-phase cells of the AI-2-deficient strain E. coli DH5α. For statistically significant differential gene expression (P < 0.05), 14 genes were induced by supernatants from a stationary culture and 6 genes were repressed, suggesting the involvement of indole (induction of tnaA and tnaL) and phosphate (repression of phoA, phoB, and phoU). To study the stability of the signals, the stationary-phase supernatant was autoclaved and was used to study its effect on E. coli gene expression. Three genes were induced by autoclaved stationary-phase supernatant, and 34 genes were repressed. In total, three genes (ompC, ptsA, and btuB) were induced and five genes (nupC, phoB, phoU, argT, and ompF) were repressed by both fresh and autoclaved stationary-phase supernatants. Furthermore, supernatant from E. coli DH5α stationary culture was found to repress E. coli K-12 AI-2 concentrations by 4.8-fold ± 0.4-fold, suggesting that an additional quorum-sensing system in E. coli exists and that gene expression is controlled as a network with different signals working at different growth stages.

Sign in / Sign up

Export Citation Format

Share Document