scholarly journals Retentive Memory of Bacteria: Long-Term Regulation of Dehalorespiration in Sulfurospirillum multivorans

2008 ◽  
Vol 191 (5) ◽  
pp. 1650-1655 ◽  
Author(s):  
Markus John ◽  
Raffael Rubick ◽  
Roland P. H. Schmitz ◽  
Jana Rakoczy ◽  
Torsten Schubert ◽  
...  
Keyword(s):  
Electron Acceptor ◽  
Initial Level ◽  
Transcript Level ◽  
Strictly Anaerobic ◽  
Terminal Electron Acceptor ◽  
Catalytically Active ◽  
Gain Energy ◽  
Pce Dehalogenase ◽  
Dehalogenase Gene

ABSTRACT The gram-negative, strictly anaerobic epsilonproteobacterium Sulfurospirillum multivorans is able to gain energy from dehalorespiration with tetrachloroethene (perchloroethylene [PCE]) as a terminal electron acceptor. The organism can also utilize fumarate as an electron acceptor. Prolonged subcultivation of S. multivorans in the absence of PCE with pyruvate as an electron donor and fumarate as an electron acceptor resulted in a decrease of PCE dehalogenase (PceA) activity. Concomitantly, the pceA transcript level equally decreased as shown by reverse transcriptase PCR. After 35 subcultivations (approximately 105 generations), a pceA transcript was not detectable and the PceA protein and activity were completely absent. In such long-term subcultivated S. multivorans cells, the biosynthesis of catalytically active PceA was restored to the initial level within about 50 h (approximately three generations) by the addition of PCE or trichloroethene. Single colonies obtained from PceA-depleted cultures were able to induce PCE dechlorination, indicating that long-term subcultured cells still contained the functional pceA gene. The results point to a novel type of long-term regulation of PCE dehalogenase gene expression in S. multivorans.

Anaerobic microorganisms and bioremediation of organohalide pollution

2015 ◽  
Vol 36 (3) ◽  
pp. 125 ◽  
Author(s):  
Matthew Lee ◽  
Chris Marquis ◽  
Bat-Erdene Judger ◽  
Mike Manefield
Keyword(s):  
Electron Acceptor ◽  
Strictly Anaerobic ◽  
Terminal Electron Acceptor ◽  
Anaerobic Microorganisms ◽  
Naturally Occurring ◽  
Subsurface Environments ◽  
Industrialised Countries

Organohalide pollution of subsurface environments is ubiquitous across all industrialised countries. Fortunately, strictly anaerobic microorganisms exist that have evolved using naturally occurring organohalides as their terminal electron acceptor. These unusual organisms are now being utilised to clean anthropogenic organohalide pollution.

scholarly journals A strictly anaerobic betaproteobacteriumGeorgfuchsia toluolicagen. nov., sp. nov. degrades aromatic compounds with Fe(III), Mn(IV) or nitrate as an electron acceptor

2009 ◽  
Vol 70 (3) ◽  
pp. 575-585 ◽  
Author(s):  
Sander A.B. Weelink ◽  
Wim van Doesburg ◽  
Flávia Talarico Saia ◽  
W. Irene C. Rijpstra ◽  
Wilfred F.M. Röling ◽  
...  
Keyword(s):  
Electron Acceptor ◽  
Aromatic Compounds ◽  
Strictly Anaerobic

Effects of terminal electron acceptor strength on film morphology and ternary memory performance of triphenylamine donor based devices

2013 ◽  
Vol 1 (24) ◽  
pp. 3816 ◽  
Author(s):  
Hao Zhuang ◽  
Qijian Zhang ◽  
Yongxiang Zhu ◽  
Xufeng Xu ◽  
Haifeng Liu ◽  
...  
Keyword(s):  
Electron Acceptor ◽  
Memory Performance ◽  
Film Morphology ◽  
Terminal Electron Acceptor

Magnetovibrio blakemorei gen. nov., sp. nov., a magnetotactic bacterium ( Alphaproteobacteria : Rhodospirillaceae ) isolated from a salt marsh

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1824-1833 ◽  
Author(s):  
Dennis A. Bazylinski ◽  
Timothy J. Williams ◽  
Christopher T. Lefèvre ◽  
Denis Trubitsyn ◽  
Jiasong Fang ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Electron Acceptor ◽  
Anaerobic Growth ◽  
Rrna Gene ◽  
Magnetotactic Bacterium ◽  
Single Chain ◽  
Circular Chromosome ◽  
Terminal Electron Acceptor ◽  
Content Type ◽  
Link Type

A magnetotactic bacterium, designated strain MV-1T, was isolated from sulfide-rich sediments in a salt marsh near Boston, MA, USA. Cells of strain MV-1T were Gram-negative, and vibrioid to helicoid in morphology. Cells were motile by means of a single polar flagellum. The cells appeared to display a transitional state between axial and polar magnetotaxis: cells swam in both directions, but generally had longer excursions in one direction than the other. Cells possessed a single chain of magnetosomes containing truncated hexaoctahedral crystals of magnetite, positioned along the long axis of the cell. Strain MV-1T was a microaerophile that was also capable of anaerobic growth on some nitrogen oxides. Salinities greater than 10 % seawater were required for growth. Strain MV-1T exhibited chemolithoautotrophic growth on thiosulfate and sulfide with oxygen as the terminal electron acceptor (microaerobic growth) and on thiosulfate using nitrous oxide (N2O) as the terminal electron acceptor (anaerobic growth). Chemo-organoautotrophic and methylotrophic growth was supported by formate under microaerobic conditions. Autotrophic growth occurred via the Calvin–Benson–Bassham cycle. Chemo-organoheterotrophic growth was supported by various organic acids and amino acids, under microaerobic and anaerobic conditions. Optimal growth occurred at pH 7.0 and 26–28 °C. The genome of strain MV-1T consisted of a single, circular chromosome, about 3.7 Mb in size, with a G+C content of 52.9–53.5 mol%.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MV-1T belongs to the family Rhodospirillaceae within the Alphaproteobacteria , but is not closely related to the genus Magnetospirillum . The name Magnetovibrio blakemorei gen. nov., sp. nov. is proposed for strain MV-1T. The type strain of Magnetovibrio blakemorei is MV-1T ( = ATCC BAA-1436T  = DSM 18854T).

Rates and potential mechanism of anaerobic nitrate-dependent microbial pyrite oxidation

2012 ◽  
Vol 40 (6) ◽  
pp. 1280-1283 ◽  
Author(s):  
Julian Bosch ◽  
Rainer U. Meckenstock
Keyword(s):  
Electron Acceptor ◽  
Pyrite Oxidation ◽  
Laboratory Data ◽  
Laboratory Studies ◽  
Mineral Phase ◽  
Terminal Electron Acceptor ◽  
Micro Organisms ◽  
Open Question ◽  
Sulfur Containing ◽  
Positive Results

Pyrite (FeS2) is a major iron- and sulfur-containing mineral phase in the environment. Oxidation of pyrite by aerobic micro-organisms has been well investigated. However, the reactivity of pyrite under anoxic conditions is still an open question. In the present paper, we summarize field and laboratory data on this chemolithotrophic respiration process with nitrate as terminal electron acceptor. Geochemical and stable isotope field data indicate that this process is occurring. Laboratory studies are more ambiguous, but recent positive results provide evidence that anaerobic microbial pyrite oxidation can, in fact, occur with nitrate as electron acceptor.

Effect of ascorbate on oxygen uptake and growth of Escherichia coli B

1988 ◽  
Vol 34 (6) ◽  
pp. 822-824 ◽  
Author(s):  
Holly E. Richter ◽  
Jacek Switala ◽  
Peter C. Loewen
Keyword(s):  
Escherichia Coli ◽  
Oxygen Uptake ◽  
Electron Acceptor ◽  
Minimal Medium ◽  
Anaerobic Growth ◽  
Rich Medium ◽  
Terminal Electron Acceptor ◽  
Subsequent Change ◽  
Escherichia Coli B

The addition of ascorbate to aerobically growing cultures of Escherichia coli B caused only a short pause in growth and no subsequent change in the rate or extent of growth. The effect of ascorbate on oxygen uptake varied from inhibition in minimal medium to stimulation in rich medium. Cyanide-resistant growth and oxygen uptake were stimulated by ascorbate. Both the rate and extent of anaerobic growth were stimulated in proportion to the amount of ascorbate added when fumarate was the terminal electron acceptor. Ascorbate had no effect on any aspect of anaerobic growth in the absence of a terminal electron acceptor or in the presence of nitrate.

scholarly journals Geobacter sulfurreducens Can Grow with Oxygen as a Terminal Electron Acceptor

2004 ◽  
Vol 70 (4) ◽  
pp. 2525-2528 ◽  
Author(s):  
W. C. Lin ◽  
M. V. Coppi ◽  
D. R. Lovley
Keyword(s):  
Electron Acceptor ◽  
Atmospheric Oxygen ◽  
Geobacter Sulfurreducens ◽  
Terminal Electron Acceptor ◽  
Anoxic Conditions ◽  
Strict Anaerobe ◽  
Subsurface Environments

ABSTRACT Geobacter sulfurreducens, previously classified as a strict anaerobe, tolerated exposure to atmospheric oxygen for at least 24 h and grew with oxygen as the sole electron acceptor at concentrations of 10% or less in the headspace. These results help explain how Geobacter species may survive in oxic subsurface environments, being poised to rapidly take advantage of the development of anoxic conditions.

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