scholarly journals fpr, a Deficient Xer Recombination Site from a Salmonella Plasmid, Fails To Confer Stability by Dimer Resolution: Comparative Studies with the pJHCMW1 mwr Site

2009 ◽  
Vol 192 (3) ◽  
pp. 883-887 ◽  
Author(s):  
Tung Tran ◽  
David J. Sherratt ◽  
Marcelo E. Tolmasky
Keyword(s):  
Central Region ◽  
Comparative Studies ◽  
Recombination Site ◽  
Binding Region ◽  
Site Specific ◽  
Site Specific Recombination ◽  
A Site

ABSTRACT Salmonella plasmid pFPTB1 includes a Tn3-like transposon and a Xer recombination site, fpr, which mediates site-specific recombination at efficiencies lower than those required for stabilizing a plasmid by dimer resolution. Mutagenesis and comparative studies with mwr, a site closely related to fpr, indicate that there is an interdependence of the sequences in the XerC binding region and the central region in Xer site-specific recombination sites.

scholarly journals Osmoregulation of Dimer Resolution at the Plasmid pJHCMW1 mwr Locus by Escherichia coli XerCD Recombination

2002 ◽  
Vol 184 (6) ◽  
pp. 1607-1616 ◽  
Author(s):  
Huong Pham ◽  
Ken J. Dery ◽  
David J. Sherratt ◽  
Marcelo E. Tolmasky
Keyword(s):  
Escherichia Coli ◽  
Central Region ◽  
High Salt ◽  
Recombination Reaction ◽  
Recombination Site ◽  
Site Specific ◽  
Site Specific Recombination ◽  
E Coli ◽  
Low Salt ◽  
Ionic Effects

ABSTRACT Xer-mediated dimer resolution at the mwr site of plasmid pJHCMW1 is osmoregulated in Escherichia coli. Whereas under low-salt conditions, the site-specific recombination reaction is efficient, under high-salt conditions, it proceeds inefficiently. Regulation of dimer resolution is independent of H-NS and is mediated by changes in osmolarity rather than ionic effects. The low level of recombination at high salt concentrations can be overcome by high levels of PepA or by mutating the ARG box to a sequence closer to the E. coli ARG box consensus. The central region of the mwr core recombination site plays a role in regulation of site-specific recombination by the osmotic pressure of the medium.

scholarly journals Amplification of the Tetracycline Resistance Determinant of pAMα1 in Enterococcus faecalis Requires a Site-Specific Recombination Event Involving Relaxase

2002 ◽  
Vol 184 (18) ◽  
pp. 5187-5193 ◽  
Author(s):  
M. Victoria Francia ◽  
Don B. Clewell
Keyword(s):  
Enterococcus Faecalis ◽  
Recombination Event ◽  
Tandem Repeats ◽  
Tetracycline Resistance ◽  
Multicopy Plasmid ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Rate Limiting Step ◽  
A Site ◽  
Rate Limiting

ABSTRACT The small multicopy plasmid pAMα1 (9.75 kb) encoding tetracycline resistance in Enterococcus faecalis is known to generate tandem repeats of a 4.1-kb segment carrying tet(L) when cells are grown extensively in the presence of tetracycline. Here we show that the initial (rate-limiting) step involves a site-specific recombination event involving plasmid-encoded relaxase activity acting at two recombination sequences (RS1 and RS2) that flank the tet determinant. We also present the complete nucleotide sequence of pAMα1.

scholarly journals Construction of an Integration-Proficient Vector Based on the Site-Specific Recombination Mechanism of Enterococcal Temperate Phage φFC1

2002 ◽  
Vol 184 (7) ◽  
pp. 1859-1864 ◽  
Author(s):  
Hee-Youn Yang ◽  
Young-Woo Kim ◽  
Hyo-Ihl Chang
Keyword(s):  
Pcr Amplification ◽  
Temperate Phage ◽  
Vector System ◽  
Recombination Mechanism ◽  
Integrase Gene ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Integration Vector ◽  
Site Specific Integration ◽  
A Site

ABSTRACT The genome of temperate phage φFC1 integrates into the chromosome of Enterococcus faecalis KBL 703 via site-specific recombination. In this study, an integration vector containing the attP site and putative integrase gene mj1 of phage φFC1 was constructed. A 2,744-bp fragment which included the attP site and mj1 was inserted into a pUC19 derivative containing the cat gene to construct pEMJ1-1. E. faecalis KBL 707, which does not contain the bacteriophage but which has a putative attB site within its genome, could be transformed by pEMJ1-1. Southern hybridization, PCR amplification, and DNA sequencing revealed that pEMJ1-1 was integrated specifically at the putative attB site within the E. faecalis KBL 707 chromosome. This observation suggested that the 2,744-bp fragment carrying mj1 and the attP site of phage φFC1 was sufficient for site-specific recombination and that pEMJ1-1 could be used as a site-specific integration vector. The transformation efficiency of pEMJ1-1 was as high as 6 × 103 transformants/μg of DNA. In addition, a vector (pATTB1) containing the 290-bp attB region was constructed. pATTB1 was transformed into Escherichia coli containing a derivative of the pET14b vector carrying attP and mj1. This resulted in the formation of chimeric plasmids by site-specific recombination between the cloned attB and attP sequences. The results indicate that the integration vector system based on the site-specific recombination mechanism of phage φFC1 can be used for genetic engineering in E. faecalis and in other hosts.

scholarly journals Site-Specific Nutrient Management for Irrigated Rice in South Central Region of Bangladesh

2018 ◽  
Vol 20 (2) ◽  
pp. 1-9
Author(s):  
MAA Mamun ◽  
SA Islam ◽  
MS Islam ◽  
AJ Mridha ◽  
MA Saleque
Keyword(s):  
Grain Yield ◽  
Central Region ◽  
Field Trial ◽  
Nutrient Management ◽  
Rice Yield ◽  
Substantial Variation ◽  
Irrigated Rice ◽  
Site Specific ◽  
South Central ◽  
A Site

A site-specific nutrient management (SSNM) field trial was conducted for irrigated rice using five fertilizer treatments: i) omission of N, ii) omission of P, iii) omission of K, iv) NPK and v) farmers’ practice (FP). Substantial variation in the native N, P, and K supply was found among farmers’ fields. The indigenous soil K produced 4.5 to 5.0 t ha-1 but native P and N gave only rice yield of 3.5 to 4.0 t ha-1. The highest grain yield (6.0 to 7.5 t ha-1) was obtained from balanced fertilization, followed by FP (4.0 to 5.0 t ha-1).The optimal grain yield at Faridpur was obtained by using N, P and K at 135, 8 and 49 kg ha-1; 139, 9 and 42 kg ha-1; and 140, 10 and 43 kg ha-1 for high, medium and low land rice, respectively. However, for Gopalgonj district fertilizer doses of N, P and K were 140, 11 and 38 kg ha-1; 142, 10 and 42 kg ha-1; and 138, 10 and 49 kg ha-1; and for Madaripur district, 126, 8 and 46 kg ha-1; 120, 7 and 38 kg ha-1; and 99, 6 and 27 kg ha-1 for high, medium and low land rice, respectively. These predicted fertilizer doses increase farmers’ income and protect environment from pollution.Bangladesh Agron. J. 2017, 20(2): 1-9

Deletion of the human T-cell receptor δ-gene by a site-specific recombination

Nature ◽  
1988 ◽  
Vol 335 (6186) ◽  
pp. 170-174 ◽  
Author(s):  
Jean-Pierre de Villartay ◽  
Richard D. Hockett ◽  
David Coran ◽  
Stanley J. Korsmeyer ◽  
David I. Cohen
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Human T Cell ◽  
A Site

scholarly journals Characterization of the Class 3 Integron and the Site-Specific Recombination System It Determines

2002 ◽  
Vol 184 (11) ◽  
pp. 3017-3026 ◽  
Author(s):  
Christina M. Collis ◽  
Mi-Jurng Kim ◽  
Sally R. Partridge ◽  
H. W. Stokes ◽  
Ruth M. Hall
Keyword(s):  
Terminal Inverted Repeat ◽  
Class 1 Integron ◽  
Recombination Mechanism ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Class 1 Integrons ◽  
Recombination System ◽  
Class 1 ◽  
A Site ◽  
Site Specific Recombination System

ABSTRACT Integrons capture gene cassettes by using a site-specific recombination mechanism. As only one class of integron and integron-determined site-specific recombination system has been studied in detail, the properties of a second class, the only known class 3 integron, were examined. The configuration of the three potentially definitive features of integrons, the intI3 gene, the adjacent attI3 recombination site, and the Pc promoter that directs transcription of the cassettes, was similar to that found in the corresponding region (5′ conserved segment) of class 1 integrons. The integron features are flanked by a copy of the terminal inverted repeat, IRi, from class 1 integrons on one side and a resolvase-encoding tniR gene on the other, suggesting that they are part of a transposable element related to Tn402 but with the integron module in the opposite orientation. The IntI3 integrase was active and able to recognize and recombine both known types of IntI-specific recombination sites, the attI3 site in the integron, and different cassette-associated 59-be (59-base element) sites. Both integration of circularized cassettes into the attI3 site and excision of integrated cassettes were also catalyzed by IntI3. The attI3 site was localized to a short region adjacent to the intI3 gene. Recombination between a 59-be and secondary sites was also catalyzed by IntI3, but at frequencies significantly lower than observed with IntI1, the class 1 integron integrase.

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