Amplification of the Tetracycline Resistance Determinant of pAMα1 in Enterococcus faecalis Requires a Site-Specific Recombination Event Involving Relaxase
2002 ◽
Vol 184
(18)
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pp. 5187-5193
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Keyword(s):
A Site
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ABSTRACT The small multicopy plasmid pAMα1 (9.75 kb) encoding tetracycline resistance in Enterococcus faecalis is known to generate tandem repeats of a 4.1-kb segment carrying tet(L) when cells are grown extensively in the presence of tetracycline. Here we show that the initial (rate-limiting) step involves a site-specific recombination event involving plasmid-encoded relaxase activity acting at two recombination sequences (RS1 and RS2) that flank the tet determinant. We also present the complete nucleotide sequence of pAMα1.
1990 ◽
Vol 172
(2)
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pp. 610-618
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2019 ◽
Vol 265
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pp. 113-116
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Keyword(s):
2002 ◽
Vol 184
(7)
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pp. 1859-1864
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Keyword(s):
2002 ◽
Vol 184
(11)
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pp. 3017-3026
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1985 ◽
Vol 82
(20)
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pp. 6990-6994
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