scholarly journals Localization of P42 and F1-ATPase  -Subunit Homolog of the Gliding Machinery in Mycoplasma mobile Revealed by Newly Developed Gene Manipulation and Fluorescent Protein Tagging

2014 ◽  
Vol 196 (10) ◽  
pp. 1815-1824 ◽  
Author(s):  
I. Tulum ◽  
M. Yabe ◽  
A. Uenoyama ◽  
M. Miyata
Keyword(s):  
Fluorescent Protein ◽  
Gene Manipulation ◽  
Atpase Subunit ◽  
F1 Atpase ◽  
Protein Tagging

scholarly journals An orange fluorescent protein tagging system for real-time pollen tracking

2013 ◽  
Vol 6 (1) ◽  
Author(s):  
J Hollis Rice ◽  
Reginald J Millwood ◽  
Richard E Mundell ◽  
Orlando D Chambers ◽  
Laura L Abercrombie ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Protein ◽  
Orange Fluorescent Protein ◽  
Protein Tagging ◽  
Tagging System

Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies

2014 ◽  
pp. 131-145
Author(s):  
Anna Doskočilová ◽  
Ivan Luptovčiak ◽  
Veronika Smékalová ◽  
Jozef Šamaj
Keyword(s):  
Fluorescent Protein ◽  
Protein Tagging

scholarly journals A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila

Fly ◽  
2019 ◽  
Vol 13 (1-4) ◽  
pp. 29-41
Author(s):  
Jun Luo ◽  
Pingping Shen ◽  
Jiong Chen
Keyword(s):  
Fluorescent Protein ◽  
Protein Tagging

scholarly journals A Simple and Efficient CRISPR Technique for Protein Tagging

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2618
Author(s):  
Fanning Zeng ◽  
Valerie Beck ◽  
Sven Schuierer ◽  
Isabelle Garnier ◽  
Carole Manneville ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Cell Types ◽  
Luciferase Assay ◽  
Primary Cells ◽  
Homology Directed Repair ◽  
Independent Gene ◽  
Protein Tagging ◽  
Selection Of

Genetic knock-in using homology-directed repair is an inefficient process, requiring the selection of few modified cells and hindering its application to primary cells. Here, we describe Homology independent gene Tagging (HiTag), a method to tag a protein of interest by CRISPR in up to 66% of transfected cells with one single electroporation. The technique has proven effective in various cell types and can be used to knock in a fluorescent protein for live cell imaging, to modify the cellular location of a target protein and to monitor the levels of a protein of interest by a luciferase assay in primary cells.

scholarly journals Gene manipulation of fluorescent protein through site-selective hydrolysis by Ce(IV)/EDTA

2005 ◽  
Vol 49 (1) ◽  
pp. 281-282
Author(s):  
Yoshihito Kitamura ◽  
Satoshi Mori ◽  
Makoto Komiyama
Keyword(s):  
Fluorescent Protein ◽  
Selective Hydrolysis ◽  
Gene Manipulation ◽  
Site Selective
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