scholarly journals Regulatory role of recF in the SOS response of Escherichia coli: impaired induction of SOS genes by UV irradiation and nalidixic acid in a recF mutant.

1987 ◽  
Vol 169 (4) ◽  
pp. 1731-1736 ◽  
Author(s):  
B Thoms ◽  
W Wackernagel
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Uv Irradiation ◽  
Sos Response ◽  
Regulatory Role

scholarly journals Regulatory Role of PlaR (YiaJ) for Plant Utilization in Escherichia coli K-12

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Tomohiro Shimada ◽  
Yui Yokoyama ◽  
Takumi Anzai ◽  
Kaneyoshi Yamamoto ◽  
Akira Ishihama
Keyword(s):  
Escherichia Coli ◽  
Genetic System ◽  
Regulatory Role ◽  
E Coli ◽  
Warm Blooded Animal ◽  
Plant Utilization ◽  
K 12

AbstractOutside a warm-blooded animal host, the enterobacterium Escherichia coli K-12 is also able to grow and survive in stressful nature. The major organic substance in nature is plant, but the genetic system of E. coli how to utilize plant-derived materials as nutrients is poorly understood. Here we describe the set of regulatory targets for uncharacterized IclR-family transcription factor YiaJ on the E. coli genome, using gSELEX screening system. Among a total of 18 high-affinity binding targets of YiaJ, the major regulatory target was identified to be the yiaLMNOPQRS operon for utilization of ascorbate from fruits and galacturonate from plant pectin. The targets of YiaJ also include the genes involved in the utilization for other plant-derived materials as nutrients such as fructose, sorbitol, glycerol and fructoselysine. Detailed in vitro and in vivo analyses suggest that L-ascorbate and α-D-galacturonate are the effector ligands for regulation of YiaJ function. These findings altogether indicate that YiaJ plays a major regulatory role in expression of a set of the genes for the utilization of plant-derived materials as nutrients for survival. PlaR was also suggested to play protecting roles of E. coli under stressful environments in nature, including the formation of biofilm. We then propose renaming YiaJ to PlaR (regulator of plant utilization).

Regulatory role of cardiolipin in the activity of an ATP-dependent protease, Lon, from Escherichia coli

2011 ◽  
Vol 149 (5) ◽  
pp. 519-527 ◽  
Author(s):  
N. Minami ◽  
T. Yasuda ◽  
Y. Ishii ◽  
K. Fujimori ◽  
F. Amano
Keyword(s):  
Escherichia Coli ◽  
Regulatory Role

scholarly journals Deuterium oxide enhances the SOS response of Escherichia coli induced by bactericidal agents

2020 ◽  
pp. 79-80
Author(s):  
С.В. Смирнова ◽  
Т.Н. Шапиро ◽  
Е.В. Игонина ◽  
С.К. Абилев
Keyword(s):  
Escherichia Coli ◽  
Dna Damage ◽  
Nalidixic Acid ◽  
Deuterium Oxide ◽  
Gene Promoter ◽  
Sos Response ◽  
Genotoxic Effect ◽  
E Coli ◽  
Lux Biosensor ◽  
First Time

Изучали генотоксическое действие бактерицидных средств диоксидина, фурацилина и налидиксовой кислоты на клетки дейтерированной культуры lux-биосенсора E.coli MG1655 (pColD::lux), люминесцирующего в результате активации промотора гена колицина colD в ответ на повреждение ДНК. Впервые показано, что оксид дейтерия (D2O) в концентрации 9% усиливает SOS-ответ, индуцированный исследуемыми лекарственными препаратами, в 1,6-2,8 раза в клетках E. coli. We studied the genotoxic effect of bactericidal agents: dioxine, furaciline and nalidixic acid on cells of the deuterated culture lux-biosensor E. coli MG1655 (pColD::lux), which luminesces as a result of activation of the colicin gene promoter colD in response to DNA damage. For the first time, it was shown that deuterium oxide (D2O) at a concentration of 9% increases the SOS response by 1.6-2.8 times in E. coli cells induced by the studied drugs.

Role of the outer membrane in the accumulation of quinolones bySerratia marcescens

2000 ◽  
Vol 46 (8) ◽  
pp. 716-722 ◽  
Author(s):  
Mercedes Berlanga ◽  
Neus Ruiz ◽  
Jordi Hernandez-Borrell ◽  
Teresa Montero ◽  
Miguel Viñas
Keyword(s):  
Escherichia Coli ◽  
Serratia Marcescens ◽  
Outer Membrane ◽  
Nalidixic Acid ◽  
Effective Barrier ◽  
Highly Hydrophobic

Accumulation of four quinolones by Serratia marcescens was measured fluorometrically. The passage of quinolones through the outer membrane was studied in both lipopolysaccharide-deficient and porin-deficient mutants. The lipopolysaccharide (LPS) layer formed a partially effective barrier for highly hydrophobic quinolones such as nalidixic acid. Quinolones with a low relative hydrophobicity coefficient seemed to pass preferentially through the water-filled Omp3 porin channels. Results were confirmed when Omp3 was cloned in a porin-defective Escherichia coli.

The role of thiol redox systems in the response of Escherichia coli to far-UV irradiation

Microbiology ◽  
2009 ◽  
Vol 78 (3) ◽  
pp. 290-295
Author(s):  
O. N. Oktyabr’skii ◽  
V. Yu. Ushakov ◽  
N. G. Muzyka ◽  
G. V. Smirnova
Keyword(s):  
Escherichia Coli ◽  
Uv Irradiation ◽  
Redox Systems ◽  
Thiol Redox
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