scholarly journals Sequence and characterization of the bacteriophage T4 comC alpha gene product, a possible transcription antitermination factor.

1992 ◽  
Vol 174 (20) ◽  
pp. 6539-6547 ◽  
Author(s):  
B Sanson ◽  
M Uzan
Keyword(s):  
Gene Product ◽  
Bacteriophage T4 ◽  
Transcription Antitermination ◽  
Alpha Gene

scholarly journals IDENTIFICATION AND CHARACTERIZATION OF THE alc GENE PRODUCT OF BACTERIOPHAGE T4

Genetics ◽  
1984 ◽  
Vol 108 (2) ◽  
pp. 291-304
Author(s):  
Elizabeth Kutter ◽  
Rolf Drivdahl ◽  
Keith Rand
Keyword(s):  
Rna Polymerase ◽  
Gene Product ◽  
Dna Sequence ◽  
Bacteriophage T4 ◽  
Two Dimensional ◽  
Phage Dna ◽  
Precise Mapping ◽  
Identification And Characterization ◽  
Α Subunits

ABSTRACT Bacteriophage T4 infection rapidly and almost completely inhibits transcription of host and other phage DNAs. Two processes have been implicated to date in this inhibition: (1) ADP ribosylation of the α subunits of the RNA polymerase, involving gp alt (which is injected with the phage DNA) and, later, gp mod; and (2) the action of the T4 alc/unf gene product, synthesized immediately after infection. The latter unfolds the host genome and also blocks transcription of cytosine-containing DNA. Here, we describe the identification on two-dimensional polyacrylamide gels of gp alc/unf, the more precise mapping of the gene and the identification and analysis of the appropriate DNA sequence from an Unf+  alc mutant.

Sign in / Sign up

Export Citation Format

Share Document