scholarly journals OpsX from Haemophilus influenzae Represents a Novel Type of Heptosyltransferase I in Lipopolysaccharide Biosynthesis

2005 ◽  
Vol 187 (17) ◽  
pp. 6242-6247 ◽  
Author(s):  
Sabine Gronow ◽  
Werner Brabetz ◽  
Buko Lindner ◽  
Helmut Brade
Keyword(s):  
Substrate Specificity ◽  
Haemophilus Influenzae ◽  
Inner Core ◽  
Core Region ◽  
The Other ◽  
Lipopolysaccharide Biosynthesis

ABSTRACT The inner core region of the lipopolysaccharide (LPS) of Haemophilus influenzae is characterized by the presence of a phosphorylated 3-deoxy-α-d-manno-octulosonic acid (Kdo). In this study, we show that the heptosyltransferase I adding the first l-glycero-d-manno-heptose residue to this acceptor is encoded by the gene opsX, which differs in substrate specificity from the other heptosyltransferase I, known as WaaC.

The role of lex2 in lipopolysaccharide biosynthesis in Haemophilus influenzae strains RM7004 and RM153

Microbiology ◽  
2003 ◽  
Vol 149 (11) ◽  
pp. 3165-3175 ◽  
Author(s):  
Ruth Griffin ◽  
Andrew D. Cox ◽  
Katherine Makepeace ◽  
James C. Richards ◽  
E. Richard Moxon ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Inner Core ◽  
Phase Variable ◽  
Specific Monoclonal Antibody ◽  
Variable Expression ◽  
Sequence Identity ◽  
Lipopolysaccharide Biosynthesis ◽  
Serotype B ◽  
Reading Frames

The locus lex2, comprising lex2A and lex2B, contributes to the phase-variable expression of lipopolysaccharide (LPS) of Haemophilus influenzae and was found to be present in 74 % of strains investigated. lex2A contains 5′-GCAA repeats which vary in number from 4 to 46 copies between strains. The locus was cloned from the serotype b strains RM7004 and RM153 and showed >99 % nucleotide sequence identity between these strains and the published lex2 sequence. Disruption of the lex2B gene in strain RM7004 resulted in truncation of some LPS glycoforms, shown by gel fractionation, with only one glycoform reacting with a digalactoside-specific monoclonal antibody, 4C4, compared with four LPS glycoforms in the more elongated LPS of the parent strain. Mass spectrometry and NMR analyses of LPS from the lex2B mutant revealed loss of the terminal digalactoside as well as the second β-glucose extending from the first heptose of the inner core. The authors conclude that Lex2B is the β-(1-4)-glucosyltransferase that adds the second β-glucose to the first β-glucose as part of the oligosaccharide extension from the first heptose of the LPS of strain RM7004. Investigation of the expression of the lex2 locus indicated that the genes are co-transcribed and that both reading frames are required for addition of this second β-glucose in a phase-variable manner.

Experimental and simulation study of impurity transport response to RMPs in RF-heated H-mode plasmas at EAST

2021 ◽  
Vol 87 (2) ◽  
Author(s):  
Germán Vogel ◽  
Hongming Zhang ◽  
Yongcai Shen ◽  
Shuyu Dai ◽  
Youwen Sun ◽  
...  
Keyword(s):  
Inner Core ◽  
Extreme Ultraviolet ◽  
Transport Coefficients ◽  
Three Dimensional ◽  
Line Emission ◽  
Core Region ◽  
Emission Mitigation ◽  
One Dimensional ◽  
Impurity Transport ◽  
Transport Code

Spatial profiles of impurity emission measurements in the extreme ultraviolet (EUV) spectroscopic range in radiofrequency (RF)-heated discharges are combined with one-dimensional and three-dimensional transport simulations to study the effects of resonant magnetic perturbations (RMPs) on core impurity accumulation at EAST. The amount of impurity line emission mitigation by RMPs appears to be correlated with the ion Z for lithium, carbon, iron and tungsten monitored, i.e. stronger suppression of accumulation for heavier ions. The targeted effect on the most detrimental high-Z impurities suggests a possible advantage using RMPs for impurity control. Profiles of transport coefficients are calculated with the STRAHL one-dimensional impurity transport code, keeping $\nu /D$ fixed and using the measured spatial profiles of $\textrm{F}{\textrm{e}^{20 + }}$ , $\textrm{F}{\textrm{e}^{21 + }}$ and $\textrm{F}{\textrm{e}^{22 + }}$ to disentangle the transport coefficients. The iron diffusion coefficient ${D_{\textrm{Fe}}}$ increases from $1.0- 2.0\;{\textrm{m}^2}\;{\textrm{s}^{ - 1}}$ to $1.5- 3.0\;{\textrm{m}^2}\;{\textrm{s}^{ - 1}}$ from the core region to the edge region $(\rho \gt 0.5)$ after the onset of RMPs. Meanwhile, an inward pinch of iron convective velocity ${\nu _{\textrm{Fe}}}$ decreases in magnitude in the inner core region and increases significantly in the outer confined region, simultaneously contributing to preserving centrally peaked $\textrm{Fe}$ profiles and exhausting the impurities. The ${D_{\textrm{Fe}}}$ and ${\nu _{\textrm{Fe}}}$ variations lead to reduced impurity contents in the plasma. The three-dimensional edge impurity transport code EMC3-EIRENE was also applied for a case of RMP-mitigated high-Z accumulation at EAST and compared to that of low-Z carbon. The exhaust of ${\textrm{C}^{6 + }}$ toward the scrape-off layer accompanying an overall suppression of heavier ${\textrm{W}^{30 + }}$ is observed when using RMPs.

scholarly journals Revisiting the Balanced and Unbalanced Aspects of Tropical Cyclone Intensification

2017 ◽  
Vol 74 (8) ◽  
pp. 2575-2591 ◽  
Author(s):  
Junyao Heng ◽  
Yuqing Wang ◽  
Weican Zhou
Keyword(s):  
Boundary Layer ◽  
Tropical Cyclone ◽  
Inner Core ◽  
Model Simulation ◽  
Surface Friction ◽  
Core Region ◽  
Physics Model ◽  
The One ◽  
Balanced Dynamics ◽  
Balanced Solution

Abstract The balanced and unbalanced aspects of tropical cyclone (TC) intensification are revisited with the balanced contribution diagnosed with the outputs from a full-physics model simulation of a TC using the Sawyer–Eliassen (SE) equation. The results show that the balanced dynamics can well capture the secondary circulation in the full-physics model simulation even in the inner-core region in the boundary layer. The balanced dynamics can largely explain the intensification of the simulated TC. The unbalanced dynamics mainly acts to prevent the boundary layer agradient flow in the inner-core region from further intensification. Although surface friction can enhance the boundary layer inflow and make the inflow penetrate more inward into the eye region, contributing to the eyewall contraction, the net dynamical effect of surface friction on TC intensification is negative. The sensitivity of the balanced solution to the procedure used to ensure the ellipticity condition for the SE equation is also examined. The results show that the boundary layer inflow in the balanced response is very sensitive to the adjustment to inertial stability in the upper troposphere and the calculation of radial wind at the surface with relatively coarse vertical resolution in the balanced solution. Both the use of the so-called global regularization and the one-sided finite-differencing scheme used to calculate the surface radial wind in the balanced solution as utilized in some previous studies can significantly underestimate the boundary layer inflow. This explains why the boundary layer inflow in the balanced response is too weak in some previous studies.

scholarly journals The length of the dry season may be associated with leaf scleromorphism in cerrado plants

2015 ◽  
Vol 87 (3) ◽  
pp. 1691-1699 ◽  
Author(s):  
MARCELO C. SOUZA ◽  
AUGUSTO C. FRANCO ◽  
MUNDAYATAN HARIDASAN ◽  
DAVI R. ROSSATTO ◽  
JANAÍNA F. DE ARAÚJO ◽  
...  
Keyword(s):  
Soil Fertility ◽  
Specific Leaf Area ◽  
Woody Species ◽  
Dry Season ◽  
Core Region ◽  
The Other ◽  
Low Fertility ◽  
Fertility Rates ◽  
Cerrado Vegetation ◽  
Central Brazil

Despite limitations of low fertility and high acidity of the soils, the cerrado flora is the richest amongst savannas. Many cerrado woody species show sclerophyllous leaves, which might be related to the availability of water and nutrients in the soil. To better understand the function and structure of cerrado vegetation within its own variations, we compared two cerrado communities: one in its core region in central Brazil (Brasília, DF) and the other on its southern periphery (Itirapina, SP). We contrasted the length of the dry season, soil fertility rates, leaf concentrations of N, P, K, Ca and Mg and the specific leaf area (SLA) between these communities. The dry season was shorter on the periphery, where the soil was more fertile although more acidic. Plants from the periphery showed higher SLA and higher leaf concentrations of N, P, Ca and Mg. We propose that the higher SLA of plants from the periphery is related to the shorter dry season, which allows better conditions for nutrient uptake.

scholarly journals Elucidation of the Monoclonal Antibody 5G8-Reactive, Virulence-Associated Lipopolysaccharide Epitope of Haemophilus influenzae and Its Role in Bacterial Resistance to Complement-Mediated Killing

2005 ◽  
Vol 73 (4) ◽  
pp. 2213-2221 ◽  
Author(s):  
Ruth Griffin ◽  
Andrew D. Cox ◽  
Katherine Makepeace ◽  
James C. Richards ◽  
E. Richard Moxon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Haemophilus Influenzae ◽  
Bacterial Resistance ◽  
Inner Core ◽  
Phase Variation ◽  
Phase Variable ◽  
Type B ◽  
Variable Expression ◽  
Virulent Type ◽  
Unknown Structure

ABSTRACT The phase-variable locus lex2 is required for expression of a Haemophilus influenzae lipopolysaccharide (LPS) epitope of previously unknown structure. This epitope, which is reactive with monoclonal antibody (MAb) 5G8, has been associated with virulence of type b strains. When strain RM118 (from the same source as strain Rd), in which the lex2 locus and MAb 5G8 reactivity are absent, was transformed with lex2 DNA, transformants that were reactive with MAb 5G8 were obtained. Surprisingly, the 5G8 reactivity of these transformants was phase variable, although the lex2 locus lacked tetrameric repeats and was constitutively expressed. This phase variation was shown to be the result of phase-variable expression of phosphorylcholine (PCho) such that MAb 5G8 reacted only in the absence of PCho. Structural analysis showed that, compared to RM118, the lex2 transformant had acquired a tetrasaccharide, Gal-α1,4-Gal-β1,4-Glc-β1,4-Glc-β1,4, linked to the proximal heptose (HepI). A terminal GalNAc was detected in a minority of glycoforms. LPS derived from a mutant of RM7004, a virulent type b strain which naturally expresses lex2 and has LPS containing the same tetrasaccharide linked to HepI as the sole oligosaccharide extension from the inner core, confirmed that GalNAc is not a part of the MAb 5G8-reactive epitope. Thus, MAb 5G8 specifically binds to the structure Gal-α1,4-Gal-β1,4-Glc-β1,4-Glc-β attached via a 1,4 linkage to HepI of H. influenzae LPS, and we show that the ability to synthesize this novel tetrasaccharide was associated with enhanced bacterial resistance to complement-mediated killing.

Outer Membrane Protein Composition in Disease Isolates of Haemophilus influenzae : Pathogenic and Epidemiological Implications

1980 ◽  
Vol 30 (3) ◽  
pp. 709-717
Author(s):  
Marilyn R. Loeb ◽  
David H. Smith
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Haemophilus Influenzae ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Outer Membrane Proteins ◽  
Protein Composition ◽  
The Other ◽  
Major Protein ◽  
Single Strain

The outer membrane protein composition of 50 disease isolates of Haemophilus influenzae has been determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. All strains, including 28 strains of serotype b , one strain each of serotypes a, c, d, e , and f , and 17 untypable strains, had an outer membrane protein composition typical of gram-negative bacteria, i.e., these membranes contained two to three dozen proteins with four to six proteins accounting for most of their protein content. Variation in the mobility of these major outer membrane proteins from strain to strain was common but not universal; the observed patterns provided useful data and new insight into the epidemiology of type b disease. The basic findings can be summarized as follows: (i) All 50 strains possessed three proteins (one minor and two major) each having identical mobilities. The other proteins, both major and minor, varied in mobility. (ii) All type b strains possessed a fourth (major) protein of identical mobility. (iii) The 28 type b strains, on the basis of the mobility of the six major outer membrane proteins, could be divided into eight subtypes. Of all the other strains examined, both typable and untypable, only the serotype a strain belonged to one of these subtypes. (iv) The untypable strains showed considerable variation in the mobilities of their major outer membrane proteins. Of these 17 strains, 13 had an additional major outer membrane protein not present in encapsulated strains. (v) The outer membrane protein composition of a single strain remained unchanged after many passages on solid media, but varied with the growth phase. (vi) The outer membrane protein composition of isolates obtained from nine patients during an epidemic of type b meningitis varied, indicating that a single strain was not responsible for the epidemic. At least five different strains were responsible for these nine cases. (vii) Identical outer membrane protein compositions were observed in the following: in a type b strain and a mutant of this strain deficient in capsule production, indicating that the level of capsule synthesis is not obviously related to outer membrane protein composition; in type b strains isolated from different anatomic sites of patients acutely ill with meningitis, indicating that the strain associated with bacteremia is the same as that isolated from the cerebrospinal fluid; in type b strains isolated from siblings who contracted meningitis at about the same time, indicating infection with the same strain; and in type b strains isolated from the initial and repeat infection of a single patient, suggesting that reinfection was due to the same strain.

Diffuse alveolar haemorrhage secondary to haemophilus influenzae in a vaping patient

2021 ◽  
Vol 14 (6) ◽  
pp. e242701
Author(s):  
Aahd Kubbara ◽  
Feras Hawari ◽  
John Johnkoski
Keyword(s):  
Lung Cancer ◽  
Haemophilus Influenzae ◽  
Case Reports ◽  
Healthcare Providers ◽  
Community Acquired Pneumonia ◽  
The Other ◽  
Alveolar Haemorrhage ◽  
Diffuse Alveolar Haemorrhage ◽  
Life Saving ◽  
Intravenous Steroids

Diffuse alveolar haemorrhage (DAH) is known to occur from several infectious and non-infectious aetiologies. Among the infectious aetiologies, Haemophilus influenzae, an organism known to cause community-acquired pneumonia, has not been reported in association with DAH prior to this case. On the other hand, vaping, an evolving epidemic concern, has been linked to various types of lung injury, including DAH. However, DAH related to vaping is only limited to several case reports. Our case describes H. influenzae pneumonia with DAH in a patient known to have vaped until the night prior to elective lobectomy for lung cancer and developed DAH within 24 hours of hospitalisation. He subsequently recovered with treatment. DAH requires immediate recognition, and healthcare providers need to be aware that neither haemoptysis nor decrease in haemoglobin level is necessarily associated. Prior to diagnosis, empirical treatment with intravenous steroids and antibiotics can be life-saving.

Sign in / Sign up

Export Citation Format

Share Document