scholarly journals Comparison of Multilocus Sequence Typing, Pulsed-Field Gel Electrophoresis, and Antimicrobial Susceptibility Typing for Characterization of Salmonella enterica Serotype Newport Isolates

2006 ◽  
Vol 44 (7) ◽  
pp. 2449-2457 ◽  
Author(s):  
H. Harbottle ◽  
D. G. White ◽  
P. F. McDermott ◽  
R. D. Walker ◽  
S. Zhao
Keyword(s):  
Antimicrobial Susceptibility ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Salmonella Enterica ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Antimicrobial Susceptibility and Characterization of Salmonella Isolates from Processed Bison Carcasses

2006 ◽  
Vol 72 (4) ◽  
pp. 3046-3049 ◽  
Author(s):  
Qiongzhen Li ◽  
Jerod A. Skyberg ◽  
Mohamed K. Fakhr ◽  
Julie S. Sherwood ◽  
Lisa K. Nolan ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Virulence Genes ◽  
Pulsed Field Gel Electrophoresis ◽  
Cross Contamination ◽  
Class 1 Integron ◽  
Pulsed Field ◽  
Class 1

ABSTRACT Seventeen Salmonella enterica serovar Hadar isolates recovered from bison were found to possess a range of virulence genes and resistance to tetracycline, gentamicin, sulfamethoxazole, and streptomycin simultaneously. A 1-kb class 1 integron containing the aadA1 gene was identified in all isolates. Pulsed-field gel electrophoresis found that all isolates were closely related, indicating the possibility of cross-contamination during processing.

Prevalence and Characterization of Salmonella Serovars Isolated from Oysters Served Raw in Restaurants

2011 ◽  
Vol 74 (6) ◽  
pp. 1025-1029 ◽  
Author(s):  
CRYSTAL D. BRILLHART ◽  
LYNN A. JOENS
Keyword(s):  
Health Risk ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Pulsed Field Gel Electrophoresis ◽  
Salmonella Spp ◽  
Pulsed Field ◽  
Plate Counts ◽  
Salmonella Serovars

To determine if Salmonella-contaminated oysters are reaching consumer tables, a survey of raw oysters served in eight Tucson restaurants was performed from October 2007 to September 2008. Salmonella spp. were isolated during 7 of the 8 months surveyed and were present in 1.2% of 2,281 oysters tested. This observed prevalence is lower than that seen in a previous study in which U.S. market oysters were purchased from producers at bays where oysters are harvested. To test whether the process of refrigerating oysters in restaurants for several days reduces Salmonella levels, oysters were artificially infected with Salmonella and kept at 4°C for up to 13 days. Direct plate counts of oyster homogenate showed that Salmonella levels within oysters did not decrease during refrigeration. Six different serovars of Salmonella enterica were found in the restaurant oysters, indicating multiple incidences of Salmonella contamination of U.S. oyster stocks. Of the 28 contaminated oysters, 12 (43%) contained a strain of S. enterica serovar Newport that matched by pulsed-field gel electrophoresis a serovar Newport strain seen predominantly in the study of bay oysters performed in 2002. The repeated occurrence of this strain in oyster surveys is concerning, since the strain was resistant to seven antimicrobials tested and thus presents a possible health risk to consumers of raw oysters.

Characterization of Listeria monocytogenes on Retail Food from Jiangsu Province, China

2013 ◽  
Vol 781-784 ◽  
pp. 1332-1335
Author(s):  
Tao Yu
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Gel Electrophoresis ◽  
Susceptibility Testing ◽  
Pulsed Field Gel Electrophoresis ◽  
Restriction Patterns ◽  
Pulsed Field ◽  
Retail Food

Listeria monocytogenes isolates recovered from retail foods were characterized by serotyping, genotyping using pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Six serotypes were identified among the isolates and the predominant serotype was 1/2a (42, 48.8%). PFGE differentiated the 86 isolates into 23 ApaI restriction patterns. Twenty-five (29.1%) of the L.monocytogenes strains exhibited resistance to at least one antimicrobial. Resistance to ciprofloxacin (17.4% of the isolates were resistant) and resistance to tetracycline (17.4%) were observed frequently.

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