scholarly journals Comparison of microorganism detection and time-to-positivity in pediatric and standard media from three major commercial continuously monitored blood culture systems

2021 ◽  
Author(s):  
Melanie L. Yarbrough ◽  
Meghan A. Wallace ◽  
Carey-Ann D. Burnham
Keyword(s):  
Blood Culture ◽  
Detection Rate ◽  
Culture Media ◽  
Blood Cultures ◽  
Kingella Kingae ◽  
Packed Red Blood Cells ◽  
Culture Systems ◽  
Time To Positivity ◽  
Species Specific ◽  
Blood Volumes

New blood culture instrumentation and media formulations have led to improved time-to-positivity (TTP) for positive blood cultures. Data regarding the necessity of pediatric blood culture bottles with contemporary blood culture systems are sparse. We compared performance of three commercial blood culture systems, evaluating impact of blood volumes in standard and pediatric blood culture media across systems. Simulated blood cultures with packed red blood cells and three Gram-positive, four Gram-negative, and one anaerobic organism (final concentrations ranging from 0.5-19 CFU/mL blood) on the VIRTUO, VersaTREK, and Bactec FX were evaluated with FAN Plus, REDOX, and BACTEC Plus media, respectively. For each media/instrument/organism combination 1, 3, 5, and 10 mL blood volumes were evaluated in triplicate. Detection rate was not affected by blood volume. Aerobic organisms that demonstrated variable detection were Kingella kingae, Haemophilus influenzae and Neisseria meningitidis. Bacteroides fragilis was detected in 83%, 100%, and 100% of VIRTUO, VersaTREK, and Bactec anaerobic bottles. Average TTP of standard media for aerobic organisms detected on VIRTUO was decreased compared to VersaTREK (-2.3 h) and Bactec (-4.9 h). Compared to standard media, detection rate and TTP was unchanged on VIRTUO, while TTP was reduced with pediatric media for 2/8 organisms tested on Bactec and 7/8 organisms on VersaTREK, illustrating the potential benefit of pediatric media on VersaTREK or BACTEC when low blood volumes (<5 mL) are collected. These results demonstrate that TTP is decreased on the VIRTUO compared to VersaTREK and Bactec for many microorganisms associated with BSI but may have species-specific limitations.

scholarly journals Real-world Time to Positivity of 2 Widely Used Commercial Blood Culture Systems in Patients With Severe Manifestations of Sepsis: An Analysis of the FABLED Study

2020 ◽  
Vol 7 (9) ◽  
Author(s):  
Guillaume Butler-Laporte ◽  
Cedric P Yansouni ◽  
Katryn Paquette ◽  
Alexander Lawandi ◽  
Sarah N Stabler ◽  
...  
Keyword(s):  
Blood Culture ◽  
Prospective Study ◽  
Bacterial Species ◽  
Blood Cultures ◽  
Study Cohort ◽  
Analytical Methodology ◽  
Multi Centre Study ◽  
Clinical Sensitivity ◽  
Culture Systems ◽  
Time To Positivity

Abstract Background Of all microbiological tests performed, blood cultures have the most impact on patient care. Timely results are essential, especially in the management of sepsis. While there are multiple available blood culture systems on the market, they have never been compared in a prospective study in a critically ill population. Methods We performed an analysis of the FABLED study cohort to compare culture results and time to positivity (TTP) of 2 widely used blood culture systems: BacT/Alert and BACTEC. In this multisite prospective study, patients with severe manifestations of sepsis had cultures drawn before antibiotics using systematic enrollment criteria and blood drawing methodology allowing for minimization of pre-analytical biases. Results We enrolled 315 patients; 144 had blood cultures (47 positive) with BacT/Alert and 171 with BACTEC (53 positive). Patients whose blood cultures were processed using the BacT/Alert system were younger (median, 64 vs 70 years; P = .003), had a higher proportion of HIV (9.03% vs 1.75%; P = .008) and a lower qSOFA (P = .003). There were no statistically significant differences in the most commonly identified bacterial species. TTP was shorter for BACTEC (median [interquartile range {IQR}], 12.5 [10–14] hours) compared with BacT/Alert (median [IQR], 17 [14–21] hours; P &lt; .0001). Conclusions In this large prospective multi-centre study comparing the two blood culture systems among patients with severe manifestations of sepsis, and using a rigorous pre-analytical methodology, the BACTEC system yielded positive culture results 4.5 hours earlier than BacT/Alert. These results apply to commonly isolated bacteria. However, our study design did not allow direct comparison of TTP for unusual pathogens nor of clinical sensitivity between systems. More research is needed to determine the clinical implications of this finding.

scholarly journals Neither Blood Culture Positivity nor Time to Positivity is Associated with Mortality among Patients Presenting with Severe Manifestations of Sepsis: The FABLED Cohort Study

2021 ◽  
Author(s):  
Katryn Paquette ◽  
David Sweet ◽  
Robert Stenstrom ◽  
Sarah N Stabler ◽  
Alexander Lawandi ◽  
...  
Keyword(s):  
Emergency Department ◽  
Cohort Study ◽  
Blood Culture ◽  
Blood Cultures ◽  
Cell Counts ◽  
Culture Positivity ◽  
Time To Positivity ◽  
Blood Culture Positivity ◽  
Normal White Blood Cell ◽  
Support Fund

Abstract Background Sepsis is a leading cause of morbidity, mortality, and health care costs worldwide. Methods We conducted a multi-center, prospective cohort study evaluating the yield of blood cultures drawn before and after empiric antimicrobial administration among adults presenting to the emergency department with severe manifestations of sepsis (ClinicalTrials.gov: NCT01867905). Enrolled patients who had the requisite blood cultures drawn were followed for 90 days. We explored the independent association between blood culture positivity and its time to positivity in relation to 90-day mortality. Findings 325 participants were enrolled; 90-day mortality among the 315 subjects followed-up was 25·4% (80/315). Mortality was associated with age (mean age in those who died was 72·5 ±15·8 vs. 62·9 ±17·7 years among survivors, p&lt;0·0001), greater Charlson Comorbidity Index (2 (IQR 1,3) vs. 1 (IQR 0,3), p=0·008), dementia (13/80 (16·2%) vs. 18/235 (7·7%), p=0·03), cancer (27/80 (33·8%) vs. 47/235 (20·0%), p=0·015), positive qSOFA score (57/80 (71·2%) vs. 129/235 (54·9%), p=0·009), and normal white blood cell counts (25/80 (31·2%) vs. 42/235 (17·9%), p=0·02). The presence of bacteremia, persistent bacteremia after antimicrobial infusion, and shorter time to blood culture positivity were not associated with mortality. Neither the source of infection nor pathogen affected mortality. Interpretation Although severe sepsis is an inflammatory condition triggered by infection, its 90-day survival is not influenced by blood culture positivity nor its time to positivity. Funding Vancouver Coastal Health; St-Paul’s Hospital Foundation Emergency Department Support Fund; the Fonds de Recherche Santé – Québec (CPY); Intramural Research Program of the NIH, Clinical Center (AL); the Maricopa Medical Foundation

Use of simulated blood cultures for time to detection comparison between BacT/ALERT™ and BACTEC™ 9240 blood culture systems

2002 ◽  
Vol 44 (3) ◽  
pp. 235-240 ◽  
Author(s):  
Egidio Francesco Viganò ◽  
Emanuela Vasconi ◽  
Carlo Agrappi ◽  
Pierangelo Clerici
Keyword(s):  
Blood Culture ◽  
Blood Cultures ◽  
Culture Systems ◽  
Time To Detection

scholarly journals Comparative study of manual conventional blood cultures versus automated blood culture system in cases of septicemia

2021 ◽  
Vol 8 (4) ◽  
pp. 327-332
Author(s):  
Humera Qudsia Fatima Ansari ◽  
Lubna Saher ◽  
Mustafa Afzal
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Cultures ◽  
Diffusion Method ◽  
Suspected Case ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Gold Standard Method ◽  
Blood Culture System ◽  
Culture Systems

: Blood cultures are a proven gold standard method for the identification of causative agents of bloodstream infections. Identification of causative organism along with antibiotic susceptibility plays a pivotal role in proposing suitable antibiotic therapy. Automated blood culture systems show improved monitoring of blood cultures by reducing the time and by ensuring more accurate results when compared to the conventional blood culture system. To isolate the organism from given blood samples of a suspected case of septicemia and to compare the results of conventional and automated blood culture systems and to study the antimicrobial susceptibility pattern of the pathogens isolated. A prospective study of 6 months period was conducted among 100 subjects attending the Department of Microbiology in a tertiary care hospital. Subjects with symptoms and signs of septicemia were included. 25ml of venous blood was drawn aseptically from the venipuncture site, of which 5ml of blood was inoculated into 50ml of Brain Heart Infusion bottle in conventional blood culture system and 10ml each into aerobic and anaerobic BACTEC PLUS bottle in Automated blood culture system BACTEC FX40. Overall, 48% and 60% of the samples revealed positive growth by the conventional and automated blood culture system BACTEC FX40, respectively. Gram Positive Cocci were 52.08% and Gram Negative Bacilli were 47.91% isolated by conventional blood culture system, whereas automated blood culture system BACTEC FX40 isolated 45% and 55%, respectively. Isolates were detected within 24-48hrs and 12-24 hrs by conventional and automated blood culture systems, respectively. The anti-microbial susceptibility pattern of the pathogens isolated was also recorded by Kirby Bauer disc diffusion method of antimicrobial susceptiblity testing. Automated blood culture systems are a trustworthy substitute to conventional blood culture systems. The automated blood culture systems being more sensitive and rapid in detecting septicemia in subjects acts as an appropriate means for the initial identification and detection of blood pathogens and improved provision of antimicrobial therapeutic options for septic Patients especially in Critical Care and Intensive Care Units where positive culture reporting is crucial.

scholarly journals Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media

1977 ◽  
Vol 6 (1) ◽  
pp. 1-3 ◽  
Author(s):  
J Eng ◽  
E Holten
Keyword(s):  
Blood Culture ◽  
Neisseria Meningitidis ◽  
Growth Medium ◽  
Solid Medium ◽  
Culture Media ◽  
Inhibitory Effect ◽  
Blood Cultures ◽  
Nutrient Broth

The inhibitory effect of sodium polyanethol sulfonate (0.05%) upon growth of Neisseria meningitidis was found to be neutralized by adding gelatin (l.1%) to the growth medium. The neutralizing effect was demonstrated in solid medium, as well as in nutrient broth for blood cultures. The findings parallel those of Wilkins and West (6) regarding gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Peptostreptococcus anaerobius.

scholarly journals Evaluation of Optimal Blood Culture Incubation Time to Maximize Clinically Relevant Results from a Contemporary Blood Culture Instrument and Media System

2020 ◽  
Author(s):  
Eric M. Ransom ◽  
Zahra Alipour ◽  
Meghan A. Wallace ◽  
Carey-Ann D. Burnham
Keyword(s):  
Blood Culture ◽  
Incubation Time ◽  
Negative Impact ◽  
Culture Media ◽  
Detection System ◽  
Blood Cultures ◽  
Standard Interval ◽  
Media System ◽  
Blood Culture Positivity ◽  
Optimal Incubation

Timely diagnosis of microorganisms in blood cultures is necessary to optimize therapy. Although blood culture media and systems have evolved for decades, the standard interval for incubation prior to discard as negative has remained five days. Here, we evaluated the optimal incubation time for the BACT/ALERT VIRTUO blood culture detection system (bioMérieux) using FA Plus (aerobic) and FN Plus (anaerobic) resin culture bottles in routine clinical use. Following IRB approval, a retrospective review evaluated the outcomes of 158,710 bottles collected between November 2018 and October 2019. The number of positive blood bottles was 13,592 (8.6%); 99% of positive aerobic and anaerobic bottles flagged positive by 91.5 h and 108 h, respectively. The mean (median) time-to-positivity for Staphylococcus aureus was 18.4 h (15.6 h), Escherichia coli 12.3 h (9.5 h), Pseudomonas aeruginosa 22.2 h (15.9 h), and Candida spp. 48.9 h (42.9 h). Only 175 bottles (0.1% of all bottles) flagged positive after four days of incubation; 89 (51%) of these bottles grew Cutibacterium (Propionibacterium) species. Chart review of blood cultures positive after four days (96 h) rarely had clinical impact, and sometimes had a negative impact on patientcare. Finally, a seeded study of the HACEK group, historically associated with delayed blood culture positivity, demonstrated no benefit to extended incubation beyond four days. Collectively, these findings demonstrated that a four-day incubation time was sufficient for the VIRTUO system and media. Implementation of the four-day incubation time could enhance clinically relevant results by reducing recovery of contaminants and finalizing blood cultures one day earlier.

P935 Time-to-positivity of aerobic blood cultures by using BacTec 9120 blood culture system

2007 ◽  
Vol 29 ◽  
pp. S244-S245
Author(s):  
St. Fokas ◽  
Sp. Fokas ◽  
G. Altouvas ◽  
M. Tsironi ◽  
S. Kaptanis ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Cultures ◽  
Blood Culture System ◽  
Time To Positivity
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