Use of simulated blood cultures for time to detection comparison between BacT/ALERT™ and BACTEC™ 9240 blood culture systems

2002 ◽  
Vol 44 (3) ◽  
pp. 235-240 ◽  
Author(s):  
Egidio Francesco Viganò ◽  
Emanuela Vasconi ◽  
Carlo Agrappi ◽  
Pierangelo Clerici
Keyword(s):  
Blood Culture ◽  
Blood Cultures ◽  
Culture Systems ◽  
Time To Detection

scholarly journals Comparative study of manual conventional blood cultures versus automated blood culture system in cases of septicemia

2021 ◽  
Vol 8 (4) ◽  
pp. 327-332
Author(s):  
Humera Qudsia Fatima Ansari ◽  
Lubna Saher ◽  
Mustafa Afzal
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Cultures ◽  
Diffusion Method ◽  
Suspected Case ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Gold Standard Method ◽  
Blood Culture System ◽  
Culture Systems

: Blood cultures are a proven gold standard method for the identification of causative agents of bloodstream infections. Identification of causative organism along with antibiotic susceptibility plays a pivotal role in proposing suitable antibiotic therapy. Automated blood culture systems show improved monitoring of blood cultures by reducing the time and by ensuring more accurate results when compared to the conventional blood culture system. To isolate the organism from given blood samples of a suspected case of septicemia and to compare the results of conventional and automated blood culture systems and to study the antimicrobial susceptibility pattern of the pathogens isolated. A prospective study of 6 months period was conducted among 100 subjects attending the Department of Microbiology in a tertiary care hospital. Subjects with symptoms and signs of septicemia were included. 25ml of venous blood was drawn aseptically from the venipuncture site, of which 5ml of blood was inoculated into 50ml of Brain Heart Infusion bottle in conventional blood culture system and 10ml each into aerobic and anaerobic BACTEC PLUS bottle in Automated blood culture system BACTEC FX40. Overall, 48% and 60% of the samples revealed positive growth by the conventional and automated blood culture system BACTEC FX40, respectively. Gram Positive Cocci were 52.08% and Gram Negative Bacilli were 47.91% isolated by conventional blood culture system, whereas automated blood culture system BACTEC FX40 isolated 45% and 55%, respectively. Isolates were detected within 24-48hrs and 12-24 hrs by conventional and automated blood culture systems, respectively. The anti-microbial susceptibility pattern of the pathogens isolated was also recorded by Kirby Bauer disc diffusion method of antimicrobial susceptiblity testing. Automated blood culture systems are a trustworthy substitute to conventional blood culture systems. The automated blood culture systems being more sensitive and rapid in detecting septicemia in subjects acts as an appropriate means for the initial identification and detection of blood pathogens and improved provision of antimicrobial therapeutic options for septic Patients especially in Critical Care and Intensive Care Units where positive culture reporting is crucial.

scholarly journals 11. Evaluation of the Bact/alert® VIRTUO™ in Terms of Time to Detection, Performance, Workflow Efficiency and Impact on Patient Management, Compared to the BACTEC™ FX Automated Blood Culture System

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S7-S7
Author(s):  
Ana V Halperin ◽  
José Luis Cortés Cuevas ◽  
Juan Antonio Del Castillo Polo ◽  
Miriam Cuesta ◽  
Sergio Talens ◽  
...  
Keyword(s):  
Blood Culture ◽  
Large Scale ◽  
Clinical Performance ◽  
Bloodstream Infections ◽  
Blood Cultures ◽  
Causative Organism ◽  
Research Support ◽  
Hands On ◽  
Time To Detection ◽  
Research Grant

Abstract Background Bloodstream infections are associated with high rates of morbidity and mortality, therefore prompt identification and antimicrobial susceptibility testing of the causative organism(s) are critical. We compared the microbiological/clinical performance of BacT/ALERT®-VIRTUO™-(BioMerieux) to that of the BACTEC™FX-(BD) instrument, with time-to-detection (TTD, from loading into system until positivity) as the primary outcome. Secondary microbiological outcomes were positivity and contamination rates, hands-on-time, turn-around-time (TAT) and time-to-identification. Methods We performed a prospective cross-over study using blood cultures from patients (>18 years) suspected of bacteremia/fungemia, localized in different wards into two strata (Stratum-1: Emergency Department-ED-; Stratum-2: in-hospital patients). Testing was performed in BACTEC™-PlusAerobic/F and BACTEC-Lytic/10-Anaerobic/F bottles and incubated in BACTEC™FX, or BacT/ALERT®FA-Plus and FN-Plus bottles and incubated in VIRTUO™. Initially, each strata was randomly assigned to one of the incubators and then alternated every 2-weeks for 6 months (October-16th-2018 to April-16th-2019). All samples were processed in parallel with the same work-flow from the moment they were flagged positive. Maximum incubation time was 5 days. Results We included a total of 4782 extractions (9510 bottles) in VIRTUO and 5139 (10193 bottles) in BACTEC. The median age was 67 years for both groups and the samples were equally distributed for each ward (ED: VIRTUO 80.9%, BD 76.4%). The number of blood cultures with at least one positive extraction was 873(18.3%) for VIRTUO and 802(15.6%) for BACTEC (p=0.0003). TTD and proportion of aerobic/anaerobic bottles is shown in Table. Hands-on-time was reduced by 15 minutes/day when using VIRTUO. Table Conclusion We have compared on a large scale and in a “real world” setting the performance of two automatic blood culture incubators. TTD was significantly lower for the VIRTUO incubated samples, with differences in both systems depending on the type of bottle (aerobic vs. anaerobic). The number of positive results was significantly higher for the VIRTUO incubated samples, which might impact antimicrobial prescription and clinical outcomes. Disclosures Ana V. Halperin, MD, Biomérieux (Grant/Research Support) José Luis Cortés Cuevas, MD, biomerieux (Research Grant or Support)biomerieux (Research Grant or Support) Juan Antonio Del Castillo Polo, MD, Biomérieux (Research Grant or Support) Sergio Talens, n/a, Biomerieux (Research Grant or Support) Robert Birch, n/a, bioMerieux Inc. (Employee) Rafael Cantón, PharmD PhD, Biomérieux (Grant/Research Support)

scholarly journals 2178. Sensitivity of Blood Cultures in Detection of Bacteremia in Febrile Neutropenia

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S739-S739
Author(s):  
Vanisha Patel ◽  
Jose Amadeo A Ferrolino ◽  
Randall Hayden ◽  
Randall Hayden ◽  
Aditya H Gaur
Keyword(s):  
Blood Culture ◽  
Febrile Neutropenia ◽  
Positive Blood Culture ◽  
Culture Media ◽  
Febrile Episode ◽  
Advisory Board ◽  
Blood Cultures ◽  
Coagulase Negative Staphylococcus ◽  
Media Type ◽  
Time To Detection

Abstract Background Febrile neutropenia (FN) secondary to bacteremia is a treatable complication of chemotherapy that increases mortality if not promptly recognized and managed. Methods The sensitivity of blood cultures collected in pediatric oncology patients with FN was assessed and stratified based on the day of FN episode, culture media type, and the source of blood culture draw at a single US center between 2013 and 2018. Paired aerobic and lytic media bottles were inoculated with each culture draw using a weight-based volume of blood; anaerobic cultures were included with initial cultures starting in September of 2015. Results In a retrospective analysis of 10,596 patients, a total of 3,039 episodes of FN were identified. Of the FN episodes, 17.7% had at least one positive blood culture; 84.5%, 1.3%, 0.9% and 13.3% of positive cultures were collected on day 0, day 1, day 2 and ≥ day 3 of a febrile episode. Among the positive day 0 cultures, the median time to detection of an organism was 14.1 hours. Host characteristics of blood culture-positive FN episodes are summarized in Table 1. Bacteremia was identified in 537 FN cases; 18.1%, 11.9% and 2.6% of cultures were positive in only aerobic, lytic or anaerobic media cultures, respectively. The most commonly isolated organisms were Escherichia coli, coagulase-negative Staphylococcus, viridans group streptococcus, Klebsiella pneumoniae and Pseudomonas aeruginosa. Fifteen percent of infectious episodes with a positive blood culture were polymicrobial. Conclusion In summary, the study findings have important clinical implications such as emphasizing the value of day 0 cultures and highlighting the importance of routinely collecting blood cultures in more than one media type. Despite an optimized blood culture approach, less than a fifth of FN episodes had a blood culture-based diagnosis. Disclosures Randall Hayden, MD, Abbott Molecular: Advisory Board; Quidel: Advisory Board; Roche Diagnostics: Advisory Board.

scholarly journals Rapid detection of bacteraemia

2010 ◽  
Vol 31 (3) ◽  
pp. 131
Author(s):  
Graeme R Nimmo
Keyword(s):  
Blood Culture ◽  
Antimicrobial Therapy ◽  
Automated Detection ◽  
Detection Methods ◽  
Automated Identification ◽  
Initial Management ◽  
Culture Systems ◽  
Time To Detection ◽  
Phenotypic Methods ◽  
Further Development

Bacteraemic sepsis has a high mortality that can be reduced by early diagnosis and initiation of appropriate antimicrobial therapy 1. Rapid confirmation of the diagnosis and identification of the causal agent provide guidance on the adequacy and duration of antimicrobial therapy and on the need for source investigation. Clinical microbiology laboratories have rightly placed great emphasis on this aspect of their practice. As causal organisms are usually present in low titre, direct microscopy is impractical and laboratories have generally relied on culture of blood in broth, which is relatively insensitive and too slow to influence initial management. Phenotypic methods for the identification and antimicrobial susceptibility testing (AST) of isolates have progressively improved over the last two decades, but still require significant periods of incubation. Similarly, commercial blood culture systems have been refined with better systems for automated detection of growth in broth, but still require incubation for up to five days and subculture for the isolation of pathogens. Constantly monitored blood culture systems and automated identification/AST are now the norm in most clinical laboratories. Although there will undoubtedly be further development of phenotypic methods, with incremental improvements in sensitivity and time-to-detection, research and development now concentrated on molecular detection methods has the potential to result in a paradigm shift in our approach to the microbiological diagnosis of this condition.

scholarly journals Comparison of microorganism detection and time-to-positivity in pediatric and standard media from three major commercial continuously monitored blood culture systems

2021 ◽  
Author(s):  
Melanie L. Yarbrough ◽  
Meghan A. Wallace ◽  
Carey-Ann D. Burnham
Keyword(s):  
Blood Culture ◽  
Detection Rate ◽  
Culture Media ◽  
Blood Cultures ◽  
Kingella Kingae ◽  
Packed Red Blood Cells ◽  
Culture Systems ◽  
Time To Positivity ◽  
Species Specific ◽  
Blood Volumes

New blood culture instrumentation and media formulations have led to improved time-to-positivity (TTP) for positive blood cultures. Data regarding the necessity of pediatric blood culture bottles with contemporary blood culture systems are sparse. We compared performance of three commercial blood culture systems, evaluating impact of blood volumes in standard and pediatric blood culture media across systems. Simulated blood cultures with packed red blood cells and three Gram-positive, four Gram-negative, and one anaerobic organism (final concentrations ranging from 0.5-19 CFU/mL blood) on the VIRTUO, VersaTREK, and Bactec FX were evaluated with FAN Plus, REDOX, and BACTEC Plus media, respectively. For each media/instrument/organism combination 1, 3, 5, and 10 mL blood volumes were evaluated in triplicate. Detection rate was not affected by blood volume. Aerobic organisms that demonstrated variable detection were Kingella kingae, Haemophilus influenzae and Neisseria meningitidis. Bacteroides fragilis was detected in 83%, 100%, and 100% of VIRTUO, VersaTREK, and Bactec anaerobic bottles. Average TTP of standard media for aerobic organisms detected on VIRTUO was decreased compared to VersaTREK (-2.3 h) and Bactec (-4.9 h). Compared to standard media, detection rate and TTP was unchanged on VIRTUO, while TTP was reduced with pediatric media for 2/8 organisms tested on Bactec and 7/8 organisms on VersaTREK, illustrating the potential benefit of pediatric media on VersaTREK or BACTEC when low blood volumes (<5 mL) are collected. These results demonstrate that TTP is decreased on the VIRTUO compared to VersaTREK and Bactec for many microorganisms associated with BSI but may have species-specific limitations.

scholarly journals Real-world Time to Positivity of 2 Widely Used Commercial Blood Culture Systems in Patients With Severe Manifestations of Sepsis: An Analysis of the FABLED Study

2020 ◽  
Vol 7 (9) ◽  
Author(s):  
Guillaume Butler-Laporte ◽  
Cedric P Yansouni ◽  
Katryn Paquette ◽  
Alexander Lawandi ◽  
Sarah N Stabler ◽  
...  
Keyword(s):  
Blood Culture ◽  
Prospective Study ◽  
Bacterial Species ◽  
Blood Cultures ◽  
Study Cohort ◽  
Analytical Methodology ◽  
Multi Centre Study ◽  
Clinical Sensitivity ◽  
Culture Systems ◽  
Time To Positivity

Abstract Background Of all microbiological tests performed, blood cultures have the most impact on patient care. Timely results are essential, especially in the management of sepsis. While there are multiple available blood culture systems on the market, they have never been compared in a prospective study in a critically ill population. Methods We performed an analysis of the FABLED study cohort to compare culture results and time to positivity (TTP) of 2 widely used blood culture systems: BacT/Alert and BACTEC. In this multisite prospective study, patients with severe manifestations of sepsis had cultures drawn before antibiotics using systematic enrollment criteria and blood drawing methodology allowing for minimization of pre-analytical biases. Results We enrolled 315 patients; 144 had blood cultures (47 positive) with BacT/Alert and 171 with BACTEC (53 positive). Patients whose blood cultures were processed using the BacT/Alert system were younger (median, 64 vs 70 years; P = .003), had a higher proportion of HIV (9.03% vs 1.75%; P = .008) and a lower qSOFA (P = .003). There were no statistically significant differences in the most commonly identified bacterial species. TTP was shorter for BACTEC (median [interquartile range {IQR}], 12.5 [10–14] hours) compared with BacT/Alert (median [IQR], 17 [14–21] hours; P &lt; .0001). Conclusions In this large prospective multi-centre study comparing the two blood culture systems among patients with severe manifestations of sepsis, and using a rigorous pre-analytical methodology, the BACTEC system yielded positive culture results 4.5 hours earlier than BacT/Alert. These results apply to commonly isolated bacteria. However, our study design did not allow direct comparison of TTP for unusual pathogens nor of clinical sensitivity between systems. More research is needed to determine the clinical implications of this finding.

scholarly journals Time-to-detection of bacteria and yeast with the BACTEC FX versus BacT/Alert Virtuo blood culture systems

2018 ◽  
Vol 38 (3) ◽  
pp. 194-199 ◽  
Author(s):  
Ali Mohammed Somily ◽  
Hanan Ahmed Habib Babay ◽  
Armen Albert Torchyan ◽  
Samina B. Sayyed ◽  
Muhammed Absar ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture Systems ◽  
Time To Detection ◽  
Bactec Fx

Establishing a Long-Term Model for Analysis and Improvement of Underfilled Blood Culture Volumes

2018 ◽  
Vol 151 (2) ◽  
pp. 164-170 ◽  
Author(s):  
Michael Zaleski ◽  
Patrick Erdman ◽  
Joshua Adams ◽  
Amanda Michael ◽  
Abigail Rudy ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Blood Culture ◽  
False Negative ◽  
Blood Cultures ◽  
Surgical Intensive Care Unit ◽  
Surgical Intensive Care ◽  
Hospital Units ◽  
Time To Detection ◽  
Oncology Unit

Abstract Objectives Underfilling of blood culture bottles decreases the sensitivity of the culture. We attempt to increase average blood culture fill volumes (ABCFVs) through an educational program. Methods Partnerships were established with four hospital units (surgical intensive care unit [SICU], medical intensive care unit [MICU], medical intermediate care unit [MIMCU], and hematology and oncology unit [HEME/ONC]). ABCFVs were continuously tracked and communicated to each unit monthly. Educational sessions were provided to each unit. Results ABCFVs for the SICU, MICU, MIMCU, and HEME/ONC were 4.8, 5.0, 5.0, and 6.3 mL/bottle, respectively. After the final education session, the SICU, MICU, MIMCU, and HEME/ONC were able to maintain an ABCFV of 6.8, 8.1, 7.9, and 8.2 mL/bottle, respectively. Conclusions Partnering with a specific unit and providing monthly volume reports with educational sessions has a direct positive correlation on increasing ABCFVs. Increasing ABCFVs has the potential to decrease false-negative blood cultures, time to detection of positive blood cultures, and time to appropriate and specific antimicrobial therapy, as well as improve patient outcomes in high-acuity patient care units.

scholarly journals In Vitro Evaluation of BacT/Alert FA Blood Culture Bottles and T2Candida Assay for Detection of Candida in the Presence of Antifungals

2018 ◽  
Vol 56 (8) ◽  
Author(s):  
Nicholas D. Beyda ◽  
Jonathan Amadio ◽  
Jose R. Rodriguez ◽  
Karen Malinowski ◽  
Kevin W. Garey ◽  
...  
Keyword(s):  
Blood Culture ◽  
Whole Blood ◽  
Blood Cultures ◽  
Assay Sensitivity ◽  
Content Type ◽  
High Inoculum ◽  
Culture Sensitivity ◽  
Time To Detection ◽  
The Impact

ABSTRACT The T2Candida assay is a novel, non-culture-based assay for the diagnosis of candidemia directly from whole blood. The impact of antifungals on the performance of the T2Candida assay and blood culture bottles has not been well described. In this study, the performance of the T2Candida assay was compared to that of blood culture in detecting Candida spp. in spiked blood cultures with or without the presence of antifungals. Clinical bloodstream isolates of Candida spp. were inoculated into human whole blood at low (1 to 5 cells/ml) and high (10 to 50 cells/ml) concentrations with or without the presence of caspofungin and fluconazole. Time to detection (TTD) was assessed for prepared samples using BacT/Alert FA aerobic blood culture bottles or the T2Candida assay. In the absence of antifungals, T2Candida assay sensitivity was comparable to that of blood culture at both the low inoculum and the high inoculum (95% versus 97.5% and 100% versus 100%, respectively) and the assay had an average TTD that was significantly shorter (5.1 h versus 27.2 to 30 h, respectively). Neither caspofungin nor fluconazole was observed to impact the sensitivity or TTD of the T2Candida assay, while fluconazole reduced the overall blood culture sensitivity by 7.5% to 12.5% (at the low inoculum and high inoculum, respectively) and significantly increased the TTD of Candida albicans, C. tropicalis, and C. parapsilosis by 14.8 to 67 h. Neither caspofungin nor fluconazole impacted the performance of the T2Candida assay in vitro, and the assay may be useful for the diagnosis of candidemia in patients receiving antifungal therapy.

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