Definitive Identification of Laribacter hongkongensis Acquired in the United States

Laribacter hongkongensisis a potential emerging pathogen associated with community-acquired gastroenteritis and traveler's diarrhea. We report the isolation ofL. hongkongensisfrom the stool of a patient who had no history of travel outside the United States. The organism was identified by phenotypic tests, mass spectrometry, and gene sequencing.

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Mycobacterium talmoniae, a Potential Pulmonary Pathogen Isolated from Multiple Patients with Bronchiectasis in the United States, Including the First Case of Clinical Disease in a Patient with Cystic Fibrosis

Journal of Clinical Microbiology ◽

10.1128/jcm.00906-18 ◽

2018 ◽

Vol 57 (2) ◽

Cited By ~ 1

Author(s):

Ravikiran Vasireddy ◽

Sruthi Vasireddy ◽

Barbara A. Brown-Elliott ◽

Alexander L. Greninger ◽

Rebecca M. Davidson ◽

...

Keyword(s):

United States ◽

Cystic Fibrosis ◽

16S Rrna ◽

Type Strain ◽

Gene Sequencing ◽

The United States ◽

Average Nucleotide Identity ◽

Content Type ◽

First Case ◽

Multiple Patients

ABSTRACTWe characterize three respiratory isolates of the recently described speciesMycobacterium talmoniaerecovered in Texas, Louisiana, and Massachusetts, including the first case of disease in a patient with underlying cystic fibrosis. The three isolates had a 100% match toM. talmoniaeNE-TNMC-100812Tby complete 16S rRNA,rpoBregion V, andhsp65 gene sequencing. Core genomic comparisons between one isolate and the type strain revealed an average nucleotide identity of 99.8%. The isolates were susceptible to clarithromycin, amikacin, and rifabutin, while resistance was observed for tetracyclines, ciprofloxacin, and linezolid.M. talmoniaeshould be added to the list of potential pulmonary pathogens, including in the setting of cystic fibrosis.

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Serotype and Genotype (Multilocus Sequence Type) ofStreptococcus suisIsolates from the United States Serve as Predictors of Pathotype

Journal of Clinical Microbiology ◽

10.1128/jcm.00377-19 ◽

2019 ◽

Vol 57 (9) ◽

Cited By ~ 12

Author(s):

April A. Estrada ◽

Marcelo Gottschalk ◽

Stephanie Rossow ◽

Aaron Rendahl ◽

Connie Gebhart ◽

...

Keyword(s):

United States ◽

Odds Ratio ◽

Clinical Information ◽

The United States ◽

Growing Pigs ◽

Sequence Type ◽

Primary Methods ◽

Content Type ◽

History Of ◽

Sequence Types

ABSTRACTStreptococcus suisis a significant cause of mortality in piglets and growing pigs worldwide. The species contains pathogenic and commensal strains, with pathogenic strains causing meningitis, arthritis, endocarditis, polyserositis, and septicemia. Serotyping and multilocus sequence typing (MLST) are primary methods to differentiate strains, but the information is limited for strains found in the United States. The objective of this study was to characterize the diversity of 208S. suisisolates collected between 2014 and 2017 across North America (mainly the United States) by serotyping and MLST and to investigate associations between subtype and pathotype classifications (pathogenic, possibly opportunistic, and commensal), based on clinical information and site of isolation. Twenty serotypes were identified, and the predominant serotypes were 1/2 and 7. Fifty-eight sequence types (STs) were identified, and the predominant ST was ST28. Associations among serotypes, STs, and pathotypes were investigated using odds ratio and clustering analyses. Evaluation of serotype and ST with pathotype identified a majority of isolates of serotypes 1, 1/2, 2, 7, 14, and 23 and ST1, ST13, ST25, ST28, ST29, ST94, ST108, ST117, ST225, ST373, ST961, and ST977 as associated with the pathogenic pathotype. Serotypes 21 and 31, ST750, and ST821 were associated with the commensal pathotype, which is composed of isolates from farms with no known history ofS. suis-associated disease. Our study demonstrates the use of serotyping and MLST to differentiate pathogenic from commensal isolates and establish links between pathotype and subtype, thus increasing the knowledge aboutS. suisstrains circulating in the United States.

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First Report of blaVIM-4- and mcr-9-Coharboring Enterobacter Species Isolated from a Pediatric Patient

mSphere ◽

10.1128/msphere.00629-19 ◽

2019 ◽

Vol 4 (5) ◽

Cited By ~ 22

Author(s):

Kalyan D. Chavda ◽

Lars F. Westblade ◽

Michael J. Satlin ◽

Andrew C. Hemmert ◽

Mariana Castanheira ◽

...

Keyword(s):

United States ◽

Pediatric Patient ◽

The United States ◽

Regulatory Genes ◽

Colistin Resistance ◽

Content Type ◽

First Report ◽

Insertion Elements ◽

History Of ◽

Enterobacter Hormaechei

ABSTRACT An Enterobacter hormaechei isolate harboring blaVIM-4 and mcr-9 was recovered from a pediatric patient in a U.S. hospital. The blaVIM-4 and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS903 and IS1, but lacks the downstream regulatory genes (qseC and qseB) found in other isolates that harbor mcr-9. IMPORTANCE We describe the complete genome assembly and sequence of a clinical Enterobacter isolate harboring both blaVIM-4 and mcr-9 recovered from a pediatric patient in the United States with a history of travel to Egypt. Moreover, to the best of our knowledge, this is the first report of an Enterobacter isolate harboring both blaVIM-4 and mcr-9 from the United States. The blaVIM-4 and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS903 and IS1, but lacks the downstream regulatory genes (qseC and qseB) found in other isolates that harbor mcr-9.

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California's Cushing connection: Harvey Cushing trained California's first neurosurgeons

Journal of Neurosurgery ◽

10.3171/jns.2002.97.3.0728 ◽

2002 ◽

Vol 97 (3) ◽

pp. 728-735 ◽

Cited By ~ 4

Author(s):

Thomas M. Keller

Keyword(s):

United States ◽

History Of Medicine ◽

The United States ◽

Content Type ◽

Surgical Practice ◽

Harvey Cushing ◽

History Of ◽

Fine Print

✓ On September 9, 2000, California celebrated its sesquicentennial anniversary of its admission to the United States. One hundred years ago, in September of 1901, Harvey Cushing began his surgical practice in Baltimore, Maryland. The proximity of these dates presents an auspicious moment for a review of the careers of three neurosurgeons trained by Dr. Cushing—Howard Naffziger, Carl Rand, and Edward Towne—who played defining roles in establishing modern neurosurgery in the Golden State. In promulgating Cushing's surgical principles and philosophy, these physicians achieved a distinguished place in the history of medicine in California.

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PacBio Genome Sequences of Eight Escherichia albertii Strains Isolated from Humans in the United States

Microbiology Resource Announcements ◽

10.1128/mra.01663-18 ◽

2019 ◽

Vol 8 (9) ◽

Cited By ~ 2

Author(s):

Rebecca L. Lindsey ◽

Lori A. Rowe ◽

Dhwani Batra ◽

Peyton Smith ◽

Nancy A. Strockbine

Keyword(s):

United States ◽

Escherichia Coli ◽

Virulence Genes ◽

The United States ◽

Genome Sequences ◽

Emerging Pathogen ◽

Content Type ◽

E Coli

Escherichia albertii is an emerging pathogen that is closely related to Escherichia coli and can carry some of the same virulence genes as E. coli. Here, we report the release of Illumina-corrected PacBio sequences for eight E. albertii genomes.

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Clinical and Microbiologic Characteristics of Cephalosporin-Resistant Escherichia coli at Three Centers in the United States

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.05650-11 ◽

2012 ◽

Vol 56 (4) ◽

pp. 1870-1876 ◽

Cited By ~ 23

Author(s):

Yoon Soo Park ◽

Jennifer M. Adams-Haduch ◽

Kathleen A. Shutt ◽

Daniel M. Yarabinec ◽

Laura E. Johnson ◽

...

Keyword(s):

United States ◽

Escherichia Coli ◽

Empirical Therapy ◽

The United States ◽

Solid Organ ◽

Content Type ◽

Highly Active ◽

History Of ◽

Cephalosporin Resistance ◽

Intra Abdominal Infection

ABSTRACTWe investigated the clinical and microbiologic features of 300 cases of cephalosporin-resistantEscherichia coliproducing extended-spectrum β-lactamase (ESBL) or plasmid-mediated AmpC β-lactamase (pAmpC) at three medical centers in the United States. Solid-organ malignancy, connective tissue disease, and a recent history of surgery were more common among pAmpC-producing cases (n= 49), whereas urinary catheter at enrollment, diabetes, and hospitalization in the past year were more common among ESBL-producing cases (n= 233). The factors independently associated with clinical outcome were the following: the presence of cardiovascular disease (odds ratio [OR], 2.88; 95% confidence interval [CI], 1.29 to 6.43), intra-abdominal infection (OR, 6.35; 95% CI, 1.51 to 26.7), other or multiples sources of infection (OR, 8.12; 95% CI, 2.3 to 28.6), age of 65 years or greater (OR, 0.43; 95% CI, 0.2 to 0.95), favorable baseline health status (OR, 0.39; 95% CI, 0.16 to 0.95), and appropriate empirical antimicrobial therapy given in the first 72 h (OR, 0.42; 95% CI, 0.20 to 0.88). β-Lactamase genes responsible for cephalosporin resistance were identified in 291 cases. CTX-M-type ESBLs accounted for 72.0%. Of those, 88.0% were CTX-M-15. The next most common type was CMY-type pAmpC (16.7%), followed by SHV- and TEM-type ESBLs (6.3 and 1.3%, respectively). Seven cases (2.3%) had KPC-type β-lactamase. Ertapenem, imipenem, meropenem, doripenem, piperacillin-tazobactam, amikacin, nitrofurantoin, and tigecycline were highly active, with greater than 90% of the isolates being susceptible. Cefepime was less active, with only 74.2% being susceptible due to the predominance of CTX-M-15. These findings have implications in the selection of appropriate empirical therapy when infection due to cephalosporin-resistantE. coliis suspected.

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