First Report of Vancomycin-Intermediate Resistance in Sequence Type 72 Community Genotype Methicillin-Resistant Staphylococcus aureus

ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) sequence type 630 (ST630) and spa type t4549 is an emerging lineage in Nordic countries, and some representatives carry the CRISPR-Cas system. Here, the complete genome sequences of two isolates from this lineage are presented, comprising chromosomes of 2,918,239 and 2,877,083 nucleotides, respectively, and a 2,473-nucleotide plasmid carrying erm(C).

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Complete Genome Sequence of Systemically Disseminated Sequence Type 8 Staphylococcal Cassette Chromosome mec Type IVl Community-Acquired Methicillin-Resistant Staphylococcus aureus

Genome Announcements ◽

10.1128/genomea.00852-17 ◽

2017 ◽

Vol 5 (35) ◽

Cited By ~ 4

Author(s):

Junzo Hisatsune ◽

Hideharu Hagiya ◽

Sumiko Shiota ◽

Motoyuki Sugai

Keyword(s):

Staphylococcus Aureus ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Methicillin Resistant Staphylococcus Aureus ◽

Sequence Type ◽

Methicillin Resistant ◽

Content Type ◽

Staphylococcal Cassette Chromosome ◽

Epidermal Cell Differentiation

ABSTRACT Staphylococcus aureus JH4899, a community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolate collected from a patient with systematically disseminated infection, is classified as sequence type 8 and carries the staphylococcal cassette chromosome mec type IVl (SCCmecIVl). It produces TSST-1, SEC, a newly discovered enterotoxin (SE1), and epidermal cell differentiation inhibitor A (EDIN-A). Here, we present the complete genome sequence of the chromosome and a plasmid harboring the se1 and ednA genes.

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Presence of both heterogeneous vancomycin-intermediate resistance and β-lactam antibiotic-induced vancomycin resistance phenotypes is associated with the outcome in methicillin-resistant Staphylococcus aureus bloodstream infection

Scandinavian Journal of Infectious Diseases ◽

10.3109/00365548.2012.723221 ◽

2012 ◽

Vol 45 (3) ◽

pp. 203-212 ◽

Cited By ~ 11

Author(s):

Tohru Takata ◽

Motoyasu Miyazaki ◽

Maki Futo ◽

Shuji Hara ◽

Shouichi Shiotsuka ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Bloodstream Infection ◽

Methicillin Resistant Staphylococcus Aureus ◽

Vancomycin Resistance ◽

Methicillin Resistant ◽

Intermediate Resistance ◽

Lactam Antibiotic ◽

Staphylococcus Aureus Bloodstream Infection

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Pediatric pharmaceutical care with anti-infective medication in a patient with acute hematogenous osteomyelitis caused by methicillin-resistant Staphylococcus aureus

International Journal of Immunopathology and Pharmacology ◽

10.1177/2058738420925713 ◽

2020 ◽

Vol 34 ◽

pp. 205873842092571

Author(s):

Chanmei Lv ◽

Jiantao Lv ◽

Yue Liu ◽

Qifeng Liu ◽

Dongna Zou

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

First Line ◽

Methicillin Resistant ◽

First Report ◽

First Line Therapy ◽

Acute Hematogenous Osteomyelitis ◽

Serious Infections ◽

Daily Dose ◽

Red Man Syndrome

The infection of the bone marrow system caused by methicillin-resistant Staphylococcus aureus (MRSA) leads to a variety of common diseases which usually occur in children under the age of 12. Vancomycin (VCM) is the first-line therapy for MRSA-caused serious infections such as bacteremia, infective endocarditis, osteomyelitis, meningitis, pneumonia, and severe skin and soft-tissue infection (e.g. necrotizing fasciitis) with a recommended dosage of 15–20 μg/mL. In this study, we first report a case of a child with MRSA-caused osteomyelitis who was successfully cured by VCM at a concentration of 4.86 μg/mL. VCM’s clinical daily dose of more than 4 g was of concern in light of recent evidence suggesting the increased risks of nephrotoxicity and red man syndrome when Cmin ⩾15 μg/mL and doses ⩾10 mg/kg in children. As far as we know, this is the first report on the lower dose of VCM in children with MRSA osteomyelitis.

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Genome Sequence of a Recently Emerged, Highly Transmissible, Multi-Antibiotic- and Antiseptic-Resistant Variant of Methicillin-Resistant Staphylococcus aureus, Sequence Type 239 (TW)

Journal of Bacteriology ◽

10.1128/jb.01255-09 ◽

2009 ◽

Vol 192 (3) ◽

pp. 888-892 ◽

Cited By ~ 131

Author(s):

Matthew T. G. Holden ◽

Jodi A. Lindsay ◽

Craig Corton ◽

Michael A. Quail ◽

Joshua D. Cockfield ◽

...

Keyword(s):

Heavy Metals ◽

Staphylococcus Aureus ◽

Genome Sequence ◽

Resistance Genes ◽

Methicillin Resistant Staphylococcus Aureus ◽

Housekeeping Genes ◽

Sequence Type ◽

Resistant Variant ◽

Methicillin Resistant ◽

Wide Range

ABSTRACT The 3.1-Mb genome of an outbreak methicillin-resistant Staphylococcus aureus (MRSA) strain (TW20) contains evidence of recently acquired DNA, including two large regions (635 kb and 127 kb). The strain is resistant to a wide range of antibiotics, antiseptics, and heavy metals due to resistance genes encoded on mobile genetic elements and also mutations in housekeeping genes.

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Whole-Genome Analysis of Livestock-Associated Methicillin-Resistant Staphylococcus aureus Sequence Type 398 Strains Isolated From Patients With Bacteremia in China

The Journal of Infectious Diseases ◽

10.1093/infdis/jiz575 ◽

2020 ◽

Vol 221 (Supplement_2) ◽

pp. S220-S228 ◽

Cited By ~ 1

Author(s):

Hongbin Chen ◽

Yuyao Yin ◽

Xiaohua Li ◽

Shuguang Li ◽

Hua Gao ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Sequence Type ◽

Teaching Hospitals ◽

Molecular Characteristics ◽

Whole Genome Analysis ◽

Methicillin Resistant ◽

Severe Infections ◽

Type V ◽

Mrsa St398

Abstract Sequence type (ST) 398 is the most prevalent clone of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA). To evaluate the molecular characteristics and phylogeny of Chinese ST398 isolates, 4 MRSA ST398 strains and 4 methicillin-susceptible S. aureus (MSSA) ST398 strains were collected from patients with bacteremia at 6 teaching hospitals in China between 1999 and 2016. Moreover, 689 ST398 genome sequences were downloaded from the GenBank database for comparison. The 4 MRSA ST398 strains were resistant to β-lactam antibiotics, and 2 strains were also resistant to erythromycin. Among the 4 MSSA ST398 strains, 2 strains displayed multidrug resistance (MDR) and were resistant to penicillin, erythromycin, tetracycline, and gentamicin. The accessory genome of MSSA ST398 was more diverse than that of MRSA ST398. All 4 MRSA ST398 strains carried type V staphylococcal cassette chromosome mec elements; however, MSSA ST398 carried more resistance genes than MRSA ST398. These 4 MRSA ST398 strains carried hemolysin, along with virulence genes associated with immune invasion and protease. Phylogenic analysis showed that the 4 MRSA ST398 strains clustered in 1 clade. The global ST398 phylogeny showed that ST398 was divided into an animal clade and a human clade, and the ST398 strains of this study clustered in the human clade. A small number of human strains were also present in the animal clade and vice versa, suggesting transmission of ST398 between animals and humans. In conclusion, livestock-associated MRSA ST398 has caused severe infections in Chinese hospitals, and it should therefore be paid more attention to and monitored.

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