scholarly journals Diagnostic Accuracy of Presepsin (sCD14-ST) for Prediction of Bacterial Infection in Cerebrospinal Fluid Samples from Children with Suspected Bacterial Meningitis or Ventriculitis

2015 ◽  
Vol 53 (4) ◽  
pp. 1239-1244 ◽  
Author(s):  
David Stubljar ◽  
Andreja Natasa Kopitar ◽  
Mojca Groselj-Grenc ◽  
Kristina Suhadolc ◽  
Teja Fabjan ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
16S Rrna ◽  
Bacterial Meningitis ◽  
16S Rrna Gene ◽  
Diagnostic Accuracy ◽  
Bacterial Infection ◽  
Area Under The Curve ◽  
Rrna Gene ◽  
Blood Contamination ◽  
Culture Methods

Children with temporary external ventricular drains (EVD) are prone to nosocomial infections. Diagnosis of bacterial meningitis and ventriculitis in these children is challenging due to frequent blood contamination of cerebrospinal fluid (CSF) and the presence of chemical ventriculitis. The aim of this study was to compare diagnostic accuracy of presepsin (sCD14-ST), a novel biomarker of bacterial infection in CSF, to predict bacterial infection in comparison to the accuracy of established biomarkers like those demonstrated in biochemical analysis of CSF. We conducted a prospective study with 18 children with suspected bacterial meningitis or ventriculitis who had 66 episodes of disease. CSF samples were taken from external ventricular drainage. We measured presepsin in CSF, as well as CSF leukocyte count, glucose, and proteins. CSF was also taken to prove bacterial infection with culture methods or with 16S rRNA gene broad-range PCR (SepsiTest; Molzym, Germany). Infection was clinically confirmed in 57 (86%) episodes of suspected meningitis or ventriculitis. Chemical ventriculitis was diagnosed in 9 (14%) episodes of suspected meningitis or ventriculitis. Diagnostic accuracies presented as area under the curve (AUC) for sCD14-ST, leukocytes, and proteins measured in CSF were 0.877 (95% confidence interval [CI], 0.793 to 0.961), 0.798 (95% CI, 0.677 to 0.920), and 0.857 (95% CI, 0.749 to 0.964), respectively. With CSF culture, we detected bacteria in 17 samples, compared to 37 detected with broad-range PCR. It was found that presepsin was present at a significantly higher level in children with clinically proven ventriculitis than in those without meningitis or ventriculitis. Diagnostic accuracies of presepsin were superior to those of leukocytes or proteins in CSF. Presepsin-guided 16S rRNA gene PCR could be used in everyday clinical practice to improve etiological diagnosis of meningitis and ventriculitis and to prescribe more appropriate antibiotics.

scholarly journals Cerebrospinal fluid MinION sequencing of 16S rRNA gene for rapid and accurate diagnosis of bacterial meningitis

2020 ◽  
Vol 80 (4) ◽  
pp. 469-496
Author(s):  
Nguyen Thi Thu Hong ◽  
Ho Dang Trung Nghia ◽  
Tran Tan Thanh ◽  
Nguyen Phu Huong Lan ◽  
Nguyen Thi Han Ny ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
16S Rrna ◽  
Bacterial Meningitis ◽  
16S Rrna Gene ◽  
Accurate Diagnosis ◽  
Rrna Gene

scholarly journals Cerebrospinal fluid MinION sequencing of 16S rRNA gene for rapid and accurate diagnosis of bacterial meningitis

2020 ◽  
Vol 101 ◽  
pp. 180
Author(s):  
H. Nguyen ◽  
N. Ho Dang Trung ◽  
T. Tan Tran ◽  
L. Nguyen Phu Huong ◽  
N. Nguyen Thi Han ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
16S Rrna ◽  
Bacterial Meningitis ◽  
16S Rrna Gene ◽  
Accurate Diagnosis ◽  
Rrna Gene

Meningoencephalitis due to Gemella haemolysans

2014 ◽  
Vol 63 (1) ◽  
pp. 138-139 ◽  
Author(s):  
Benjamin T. Galen ◽  
David B. Banach ◽  
Melissa R. Gitman ◽  
Terence K. Trow
Keyword(s):  
Cerebrospinal Fluid ◽  
16S Rrna ◽  
Bacterial Meningitis ◽  
16S Rrna Gene ◽  
Potential Role ◽  
Invasive Disease ◽  
Rrna Gene ◽  
Cerebrospinal Fluid Culture ◽  
Culture Negative ◽  
Fluid Culture

Gemella haemolysans is an uncommon but described cause of invasive disease in humans. We report a case of meningitis due to G. haemolysans that did not grow in cerebrospinal fluid culture, demonstrating a potential role for direct 16S rRNA gene PCR and sequencing in culture-negative cerebrospinal fluid when bacterial meningitis is suspected.

scholarly journals Bacillus massiliensis sp. nov., isolated from cerebrospinal fluid

2006 ◽  
Vol 56 (7) ◽  
pp. 1485-1488 ◽  
Author(s):  
Olga O. Glazunova ◽  
Didier Raoult ◽  
Véronique Roux
Keyword(s):  
Cerebrospinal Fluid ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Bacillus Sphaericus ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics

An unidentified Gram-negative-staining, aerobic, rod-shaped, spore-forming bacterium was isolated from a sample of cerebrospinal fluid. Based on comparative analysis of 16S rRNA gene sequences and phenotypic characteristics, the novel isolate was included in the Bacillus sphaericus-like group. The isolate was closely related to Bacillus odysseyi and Bacillus silvestris, with 96.2 and 94.4 % 16S rRNA gene sequence similarity, respectively. The major fatty acid was iso-C15 : 0 (48 %). The name Bacillus massiliensis sp. nov. is proposed for the novel isolate, with strain 4400831T (=CIP 108446T=CCUG 49529T) as the type strain.

scholarly journals Diagnostic Utility of Broad Range Bacterial 16S rRNA Gene PCR with Degradation of Human and Free Bacterial DNA in Bloodstream Infection Is More Sensitive Than an In-House Developed PCR without Degradation of Human and Free Bacterial DNA

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Petra Rogina ◽  
Miha Skvarc ◽  
David Stubljar ◽  
Romina Kofol ◽  
Achim Kaasch
Keyword(s):  
Blood Culture ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Bacterial Infection ◽  
Blood Stream Infection ◽  
Diagnostic Algorithm ◽  
Rrna Gene ◽  
Suspected Sepsis ◽  
Bacterial Dna

We compared a commercial broad range 16S rRNA gene PCR assay (SepsiTest) to an in-house developed assay (IHP). We assessed whether CD64 index, a biomarker of bacterial infection, can be used to exclude patients with a low probability of systemic bacterial infection. From January to March 2010, 23 patients with suspected sepsis were enrolled. CD64 index, procalcitonin, and C-reactive protein were measured on admission. Broad range 16S rRNA gene PCR was performed from whole blood (SepsiTest) or blood plasma (IHP) and compared to blood culture results. Blood samples spiked withStaphylococcus aureuswere used to assess sensitivity of the molecular assaysin vitro. CD64 index was lower in patients where possible sepsis was excluded than in patients with microbiologically confirmed sepsis (P=0.004). SepsiTest identified more relevant pathogens than blood cultures (P=0.008); in three patients (13%) results from blood culture and SepsiTest were congruent, whereas in four cases (17.4%) relevant pathogens were detected by SepsiTest only.In vitrospiking experiments suggested equal sensitivity of SepsiTest and IHP. A diagnostic algorithm using CD64 index as a decision maker to perform SepsiTest shows improved detection of pathogens in patients with suspected blood stream infection and may enable earlier targeted antibiotic therapy.

scholarly journals Periductal Mastitis: An Inflammatory Disease Related to Bacterial Infection and Consequent Immune Responses?

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Lu Liu ◽  
Fei Zhou ◽  
Pin Wang ◽  
Lixiang Yu ◽  
Zhongbing Ma ◽  
...  
Keyword(s):  
Risk Factors ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Bacterial Infection ◽  
Inflammatory Disease ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Rrna Gene Sequencing ◽  
Periductal Mastitis

Periductal mastitis (PDM) is a prolonged inflammatory disease, but the cause of PDM is poorly understood. In the present case control study, 87 PDM and 87 healthy controls were enrolled and the results were evaluated to identify the significant risk factors for PDM. To investigate the roles of bacterial infection and critical cytokines expression, 16S rRNA gene sequencing and bacterial culturing were conducted. We also measured the levels of interferon-γ, interleukin-12A, and interleukin-17A by semiquantitative immunohistochemistry method. In a multivariable logistic regression model, we identified overweight/obesity and late onset of menarche as independent risk factors for PDM. In contrast, age of first birth >27 years had a protective effect. With 16S rRNA gene sequencing, we confirmed bacterial infections were found in all PDM patients, but none of the control patients was positive on the gene expression of 16S rRNA. Our results also demonstrated significant increases of the IFN-γ and IL-12A expression in PDM, but there was no difference in IL-17A expression in these two groups. Taken together, this study suggests that reproductive factors and overweight/obesity are possible predisposing risk factors for PDM. Bacterial infection and the increased expression of some proinflammatory cytokines are associated with the pathogenesis of this disease.

scholarly journals Comprehensive Analysis of Bacterial Flora in Postoperative Maxillary Cyst Fluid by 16S rRNA Gene and Culture Methods

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Naoto Sano ◽  
Yoshio Yamashita ◽  
Kazumasa Fukuda ◽  
Hatsumi Taniguchi ◽  
Masaaki Goto ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bacterial Species ◽  
Bacterial Flora ◽  
Gene Analysis ◽  
Rrna Gene ◽  
Culture Methods ◽  
16S Rrna Gene Analysis ◽  
Staphylococcus Capitis ◽  
Maxillary Cyst

Intracystic fluid was aseptically collected from 11 patients with postoperative maxillary cyst (POMC), and DNA was extracted from the POMC fluid. Bacterial species were identified by sequencing after cloning of approximately 580 bp of the 16S rRNA gene. Identification of pathogenic bacteria was also performed by culture methods. The phylogenetic identity was determined by sequencing 517–596 bp in each of the 1139 16S rRNA gene clones. A total of 1114 clones were classified while the remaining 25 clones were unclassified. A total of 103 bacterial species belonging to 42 genera were identified in POMC fluid samples by 16S rRNA gene analysis. Species of Prevotella (91%), Neisseria (73%), Fusobacterium (73%), Porphyromonas (73%), and Propionibacterium (73%) were found to be highly prevalent in all patients. Streptococcus mitis (64%), Fusobacterium nucleatum (55%), Propionibacterium acnes (55%), Staphylococcus capitis (55%), and Streptococcus salivarius (55%) were detected in more than 6 of the 11 patients. The results obtained by the culture method were different from those obtained by 16S rRNA gene analysis, but both approaches may be necessary for the identification of pathogens, especially of bacteria that are difficult to detect by culture methods, and the development of rational treatments for patients with POMC.

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