scholarly journals Demonstration of the capsular antigens of bovine group B streptococci by the serum-soft agar method

1975 ◽  
Vol 2 (1) ◽  
pp. 35-41
Author(s):  
J A Kane ◽  
A E Rabkin ◽  
W W Karakawa
Keyword(s):  
Effective Means ◽  
Bovine Mastitis ◽  
Preliminary Evidence ◽  
Soft Agar ◽  
Chemical Analyses ◽  
Compact Type ◽  
Group B Streptococci ◽  
Group B ◽  
Capsular Antigens ◽  
Capsular Material

The elaboration of type-specific capsular antigens by group B streptococci can be demonstrated by the serum-soft agar technique. Group B streptococci isolated from bovine mastitis, namely, strains 9F, 14Mi, 8Mo, 44B, and 4S, were shown to form diffuse and compact types of colony morphology in serum-soft agar. Immunochemical and chemical analyses of antigens isolated from diffuse and compact colonies of strain 9F indicated that the diffuse-type growth of this strain was due to the elaboration of a galactose-rich surface antigen, whereas the compact 9F strain was devoid of this antigen. Specific 9F antiserum was effective in converting the diffuse 9F colonies of the compact type, indicating the presence of capsular material. Preliminary evidence suggests that the serum-soft agar technique could also be used to determine the antigenic diversity of the surface antigens of group B streptococci, thus providing an effective means of typing those organisms.

scholarly journals Identification of Staphylococcus aureus and Group B Streptococci from Bovine Mastitis Cases by Latex Agglutination

1995 ◽  
Vol 48 (1) ◽  
pp. 13-17
Author(s):  
Ichiro NAKAYA ◽  
Etsuji HAKOGI ◽  
Yoshiyuki MARUO ◽  
Yuji OGURA ◽  
Susumu NOMA ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Mastitis ◽  
Latex Agglutination ◽  
Group B Streptococci ◽  
Group B

Isolation, Antibiogram and Molecular Characterization of Group B Streptococci Isolates from Bovine Mastitis

2021 ◽  
Author(s):  
Rajwent Singh ◽  
A.K. Arora ◽  
T.S. Rai ◽  
Mudit Chandra
Keyword(s):  
Genetic Diversity ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Bovine Mastitis ◽  
Marker Genes ◽  
Group B Streptococci ◽  
Milk Samples ◽  
Development Of Resistance ◽  
Group B

Background: Group B streptococcus (GBS) or Streptococcus agalactiae is an important pathogen associated with bovine mastitis. The organism is also of public health consequences and may cause variety of infections ranging from neonatal sepsis, pneumonia and meningitis to localized infections and urinary tract infection or arthritisin adult humans. Widespread use of antibiotics in veterinary medicine has led to development of resistance among the pathogens. So there is need for surveillance of antimicrobial resistance to ensure effective treatment. Methods: Milk samples collected from mastitis affected animals were processed for isolation of Streptococcus agalactiae. The isolates were tested for antimicrobial susceptibility. Molecular characterisation was carried out by PCR to study the occurrence of resistance marker genes and virulence marker genes. RAPD was carried out to study genetic diversity among the isolates. Result: Six isolates of S. agalactiae were obtained from 182 milk samples. Highest resistance was observed against co-trimoxazole and tetracycline followed by ampicillin. tetM gene and tetO genes could be amplified in four and three isolates, respectively. None of the isolates showed amplification for ermA, ermB, mefA and mefE genes. Three isolates were positive for the five virulence genes tested (glnA, cfb, hylB, scaA and cyl). RAPD analysis demonstrated great intraspecific genetic diversity among the streptococcal isolates.

scholarly journals A haemagglutinating adhesin of group B streptococci isolated from cases of bovine mastitis mediates adherence to HeLa cells

1993 ◽  
Vol 139 (9) ◽  
pp. 2173-2178 ◽  
Author(s):  
I W. T. Wibawan ◽  
C. Lammler ◽  
R. S. Seleim ◽  
F. H. Pasaribu
Keyword(s):  
Hela Cells ◽  
Bovine Mastitis ◽  
Group B Streptococci ◽  
Group B

Susceptibility to Six Antibiotics of Group B Streptococci Isolated from Cerebrospinal Fluid

1985 ◽  
Vol 17 (2) ◽  
pp. 191-193
Author(s):  
Chris Mulder ◽  
Pieter Bol ◽  
Arjan Nabbe ◽  
Bob Zanen
Keyword(s):  
Cerebrospinal Fluid ◽  
Group B Streptococci ◽  
Group B

scholarly journals Influence of group B streptococci on piglet pulmonary artery response to bradykinin

1999 ◽  
Vol 86 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Richard M. Whitehurst ◽  
Rachel Laskey ◽  
Ronald N. Goldberg ◽  
Donald Herbert ◽  
Cornelius Van Breemen
Keyword(s):  
Pulmonary Artery ◽  
Contractile Response ◽  
Isometric Force ◽  
Control Group ◽  
Relaxed Controls ◽  
Vascular Response ◽  
Group B Streptococci ◽  
Resting Tension ◽  
Neonatal Group ◽  
Group B

To study whether a sepsis-induced increase in des-Arg9-bradykinin (des-Arg9-BK) and bradykinin (BK) B1-receptor activity participates in the observed increase in pulmonary vascular resistance in neonatal group B streptococcal sepsis (GBS), isometric force bioassays of pulmonary artery (PA) rings were studied, after 4-h exposure to either Krebs or GBS, by using the following protocols: 1) BK dose-response curve, 2) vascular response to BK with N G-nitro-l-arginine methyl ester (l-NAME), and 3) response to des-Arg9-BK (BK metabolite and B1 agonist). PA rings exposed to BK resulted in contraction in the GBS group and a decrease in resting tension in the Control group ( P = 0.034) at a concentration of 10−5 M. GBS-treated PA rings contracted more to des-Arg9-BK than did Controls ( P < 0.001). BK (10−6 M) relaxed preconstricted PA rings incubated in GBS less than BK relaxed Controls ( P < 0.001), and preincubation withl-NAME decreased relaxation in both. These results suggest that GBS decreased endothelium-dependent BK relaxation and increased contractile response to des-Arg9-BK. We speculate that this occurs secondary to upregulation of B1 receptors reflected by B1-agonist-mediated PA contraction.

Sign in / Sign up

Export Citation Format

Share Document