scholarly journals Identification of Staphylococcus aureus and Group B Streptococci from Bovine Mastitis Cases by Latex Agglutination

1995 ◽  
Vol 48 (1) ◽  
pp. 13-17
Author(s):  
Ichiro NAKAYA ◽  
Etsuji HAKOGI ◽  
Yoshiyuki MARUO ◽  
Yuji OGURA ◽  
Susumu NOMA ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Mastitis ◽  
Latex Agglutination ◽  
Group B Streptococci ◽  
Group B

scholarly journals Demonstration of the capsular antigens of bovine group B streptococci by the serum-soft agar method

1975 ◽  
Vol 2 (1) ◽  
pp. 35-41
Author(s):  
J A Kane ◽  
A E Rabkin ◽  
W W Karakawa
Keyword(s):  
Effective Means ◽  
Bovine Mastitis ◽  
Preliminary Evidence ◽  
Soft Agar ◽  
Chemical Analyses ◽  
Compact Type ◽  
Group B Streptococci ◽  
Group B ◽  
Capsular Antigens ◽  
Capsular Material

The elaboration of type-specific capsular antigens by group B streptococci can be demonstrated by the serum-soft agar technique. Group B streptococci isolated from bovine mastitis, namely, strains 9F, 14Mi, 8Mo, 44B, and 4S, were shown to form diffuse and compact types of colony morphology in serum-soft agar. Immunochemical and chemical analyses of antigens isolated from diffuse and compact colonies of strain 9F indicated that the diffuse-type growth of this strain was due to the elaboration of a galactose-rich surface antigen, whereas the compact 9F strain was devoid of this antigen. Specific 9F antiserum was effective in converting the diffuse 9F colonies of the compact type, indicating the presence of capsular material. Preliminary evidence suggests that the serum-soft agar technique could also be used to determine the antigenic diversity of the surface antigens of group B streptococci, thus providing an effective means of typing those organisms.

Cross protection in mice with the Smith diffuse strain of Staphylococcus aureus versus a type Ia strain of group B streptococci

1982 ◽  
Vol 28 (7) ◽  
pp. 726-732 ◽  
Author(s):  
Yoshitoshi Ichiman ◽  
Kosaku Yoshida
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Surface ◽  
Active Immunization ◽  
Cell Vaccine ◽  
Group B Streptococci ◽  
Type Ia ◽  
Whole Cell Vaccine ◽  
Surface Polysaccharides ◽  
Surface Polysaccharide ◽  
Group B

Active immunization of mice with whole cell vaccine or cell surface polysaccharide from either the Smith diffuse strain of Staphylococcus aureus or SS-615 (type Ia of group B streptococci) protected against challenge by either the homologous or heterologous strains. In the peritoneal cavity of mice immunized with either of these organisms rapid phagocytosis and reduction of the viable cells was observed at 6 h after the challenge. Cell surface polysaccharides extracted from strains Smith diffuse and SS-615, both prepared by the same procedure as that of the Smith surface antigen, were capable of absorbing the protective antibody in rabbit hyperimmune sera prepared with homologous or heterologous strains.

scholarly journals Analysis of the microbial flora in breast abscess: a retrospective cohort study conducted in the emergency department

2017 ◽  
Vol 4 (7) ◽  
pp. 2143 ◽  
Author(s):  
Ramya Ramakrishnan ◽  
Ramakrishnan V. Trichur ◽  
Sowmya Murugesan ◽  
Srihari Cattamanchi
Keyword(s):  
Staphylococcus Aureus ◽  
Cohort Study ◽  
Retrospective Cohort Study ◽  
Retrospective Cohort ◽  
Microbial Flora ◽  
Breast Abscess ◽  
Group B Streptococci ◽  
Culture Sensitivity ◽  
Group B ◽  
Age Range

Background: Breast abscess develops as a complication of lactational mastitis. Recently, there is an increase in the incidence of non-lactational breast abscess. The aim of the study was to analyse the microbial flora in the lactational and non-lactational breast abscess in the ED and to elucidate the susceptibility of flora to different antibiotics.Methods: This is a retrospective cohort study of breast abscess patients registered in the ED over two years. The case records of these patients were retrieved, and the details of their age, clinical presentation, investigation, and treatment were recorded. Specifically, the pus culture sensitivity and antibiotics used in the management of these patients were noted, and the pattern of microbiological flora analyzed.Results: A total of 124 patients were included in the study, with 97 women were categorized into lactational breast abscess, and 27 women were classified as a non-lactational breast abscess. Mean age was 24 years of age (Range 18 to 56 years). The majority of the women were young between 18 and 34 years of age (83%). The culture was grown in 92% of the patient pus specimens. Staphylococcus aureus (83%) was common organisms cultured in lactational breast abscess. Mixed Flora including Staphylococcus aureus and Group B Streptococci were grown in non-lactational breast abscess.Conclusions: Appropriate antibiotic choices are of immense importance in the management of breast abscess. Mixed flora is common in non - lactational breast abscess when compared with a lactational breast abscess. Staphylococcus Aureus is the most common isolate in both groups.

Isolation, Antibiogram and Molecular Characterization of Group B Streptococci Isolates from Bovine Mastitis

2021 ◽  
Author(s):  
Rajwent Singh ◽  
A.K. Arora ◽  
T.S. Rai ◽  
Mudit Chandra
Keyword(s):  
Genetic Diversity ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Bovine Mastitis ◽  
Marker Genes ◽  
Group B Streptococci ◽  
Milk Samples ◽  
Development Of Resistance ◽  
Group B

Background: Group B streptococcus (GBS) or Streptococcus agalactiae is an important pathogen associated with bovine mastitis. The organism is also of public health consequences and may cause variety of infections ranging from neonatal sepsis, pneumonia and meningitis to localized infections and urinary tract infection or arthritisin adult humans. Widespread use of antibiotics in veterinary medicine has led to development of resistance among the pathogens. So there is need for surveillance of antimicrobial resistance to ensure effective treatment. Methods: Milk samples collected from mastitis affected animals were processed for isolation of Streptococcus agalactiae. The isolates were tested for antimicrobial susceptibility. Molecular characterisation was carried out by PCR to study the occurrence of resistance marker genes and virulence marker genes. RAPD was carried out to study genetic diversity among the isolates. Result: Six isolates of S. agalactiae were obtained from 182 milk samples. Highest resistance was observed against co-trimoxazole and tetracycline followed by ampicillin. tetM gene and tetO genes could be amplified in four and three isolates, respectively. None of the isolates showed amplification for ermA, ermB, mefA and mefE genes. Three isolates were positive for the five virulence genes tested (glnA, cfb, hylB, scaA and cyl). RAPD analysis demonstrated great intraspecific genetic diversity among the streptococcal isolates.

scholarly journals Genital Flora during Pregnancy and Colonization of the Newborn

1980 ◽  
Vol 73 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Jean M Ross ◽  
J R Needham
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pregnant Women ◽  
Isolation Rate ◽  
Group B Streptococci ◽  
Vaginal Microflora ◽  
Postnatal Mothers ◽  
Similar Range ◽  
Group B ◽  
And Staphylococcus Aureus

A longitudinal study of the cervical and vaginal microflora of 131 pregnant women showed a similar range of organisms at first visit (up to 16 weeks), at 28 weeks and at 36 weeks of pregnancy. Twenty different groups or genera of microorganisms were recovered, predominantly lactobacilli. There was a fall in the isolation rate of organisms in the mothers 6 to 8 weeks after delivery. Lactobacilli and yeasts including Candida albicans were recovered less frequently whereas the incidence of Escherichia coli and group B streptococci increased. Infants bom to these mothers were swabbed within 24 hours of delivery and yielded organisms from the umbilicus, ear and mouth in 24%, 33% and 38% of cases respectively. Alpha haemolytic streptococci and Staphylococcus aureus were the predominant organisms. Sixteen different groups or genera were isolated. The isolation rates in non-pregnant women attending a ‘well-women’ clinic were similar to those in the postnatal mothers; few women of 60 years or more were colonized.

A novel pcr format for screening of methicillin-resistant Staphylococcus aureus and group b streptococci in swabs

Pathology ◽  
2019 ◽  
Vol 51 ◽  
pp. S138-S139
Author(s):  
Christopher J. McIver ◽  
Ranapanada Nilushika D. De Silva ◽  
Noel Er ◽  
Ryanbi Pratama ◽  
Chinmoy Mukerjee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Group B Streptococci ◽  
Methicillin Resistant ◽  
Group B

Enterotoxin Production by Staphylococcus aureus Isolated from Mastitic Cows

2003 ◽  
Vol 66 (9) ◽  
pp. 1693-1696 ◽  
Author(s):  
B. T. CENCI-GOGA ◽  
M. KARAMA ◽  
P. V. ROSSITTO ◽  
R. A. MORGANTE ◽  
J. S. CULLOR
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Products ◽  
Bovine Mastitis ◽  
Food Poisoning ◽  
Dairy Farms ◽  
Latex Agglutination ◽  
San Joaquin Valley ◽  
In Vitro Synthesis ◽  
Milk And Dairy Products

Staphylococcus aureus is an important cause of mastitis in cows. The ability of S. aureus strains to produce one or more enterotoxins in milk and dairy products is linked to staphylococcal food poisoning. To determine whether staphylococci causing bovine mastitis could cause human foodborne intoxication, the production of staphylococcal enterotoxins A through D (SEA, SEB, SEC, and SED) by 160 S. aureus isolates was evaluated with the use of a reverse passive latex agglutination enterotoxin kit. All S. aureus strains were isolated over a 9-month period from 2,343 routine submissions of a composite quarter collection of individual mastitic cows at 18 dairy farms in the San Joaquin Valley in California. Prior to enterotoxin detection, isolates were grown by a method that enhances the in vitro synthesis of enterotoxin. Twenty-two of 160 S. aureus isolates produced enterotoxin. Seven produced SEC, 12 produced SED, and 3 produced both SEC and SED. None of the isolates produced SEA or SEB.

scholarly journals Rapid slide coagglutination test for identifying and typing group B streptococci

1977 ◽  
Vol 6 (3) ◽  
pp. 266-270
Author(s):  
M K Kirkegaard ◽  
C R Field
Keyword(s):  
Staphylococcus Aureus ◽  
Blind Study ◽  
Blood Agar ◽  
Acid Extract ◽  
Group B Streptococci ◽  
Type Group ◽  
Precipitin Test ◽  
Group B

A Cowan I strain of Staphylococcus aureus was labeled with either group B streptococcal grouping or typing antiserum. These antibody-labeled reagent cells (ARC) were used in a slide coagglutination test to identify and type group B streptococci from blood agar plates. All streptococci were also identified by the standard Lancefield capillary precipitin test. In a blind study, all 141 group B streptococci were correctly identified by the coagglutination grouping test. None of the 148 non-group B streptococci caused agglutination of ARC. The coagglutination grouping test required an acid extract prepared from only four colonies and could be completed less than 30 min after colonies were removed from plates. The coagglutination typing test correctly identified 98.6% of the types of the 141 group B streptococcal strains tested. At least 88.6% of these streptococci could be typed directly from blood agar plates within 5 min by the coagglutination typing test. The remaining 11.4% of the group B streptococci were acid extracted (less than a 30-min procedure), and the extract was used for coagglutination typing. Coagglutination typing can be performed with only four colonies. The coagglutination grouping and typing tests are inexpensive, rapid, reliable, and easy to perform.

Sign in / Sign up

Export Citation Format

Share Document