scholarly journals Purification of a baculovirus-expressed hepatitis E virus structural protein and utility in an enzyme-linked immunosorbent assay.

1995 ◽  
Vol 33 (12) ◽  
pp. 3308-3311 ◽  
Author(s):  
J He ◽  
W M Ching ◽  
P Yarbough ◽  
H Wang ◽  
M Carl
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Structural Protein

scholarly journals Good Performance of Immunoglobulin M Assays in Diagnosing Genotype 3 Hepatitis E Virus Infections

2009 ◽  
Vol 16 (5) ◽  
pp. 772-774 ◽  
Author(s):  
Florence Legrand-Abravanel ◽  
Isabelle Thevenet ◽  
Jean-Michel Mansuy ◽  
Karine Saune ◽  
Françoise Vischi ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis E Virus ◽  
Rapid Test ◽  
Hepatitis E ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Genotype 3

ABSTRACT We have evaluated three anti-hepatitis E virus (anti-HEV) immunoglobulin M (IgM) assays, the EIAgen HEV IgM assay (Adaltis), the HEV IgM enzyme-linked immunosorbent assay 3.0, and the Assure HEV IgM rapid test (MP Diagnostics), for the routine detection of acute genotype 3 HEV. Their sensitivities were fairly good (90%, 88%, and 82%), and their specificities were excellent (100%, 99.5%, and 100%).

scholarly journals Comparison of a New Immunochromatographic Test to Enzyme-Linked Immunosorbent Assay for Rapid Detection of Immunoglobulin M Antibodies to Hepatitis E Virus in Human Sera

2005 ◽  
Vol 12 (5) ◽  
pp. 593-598 ◽  
Author(s):  
Hsiao Ying Chen ◽  
Yang Lu ◽  
Teresa Howard ◽  
David Anderson ◽  
Priscilla Yiquan Fong ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Immunosorbent Assay ◽  
Predictive Value ◽  
Rapid Detection ◽  
Hepatitis E Virus ◽  
Rapid Test ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Acute Hepatitis E

ABSTRACT An immunochromatographic test for rapid detection of IgM antibodies in patients with acute hepatitis E infection was developed utilizing the well-characterized recombinant protein EP2.1 and monoclonal antibody 4B2. The new rapid test based on a novel reverse-flow technology was able to generate a positive result within 2 to 3 min. Our study showed that this test was able to detect anti-HEV IgM antibodies in 96.7% of the patient samples tested (n = 151) while maintaining an excellent specificity of 98.6% with samples from various patient or healthy control groups (total n = 208). Furthermore, this rapid test gave a good specificity of 90.9% when tested with rheumatoid factor (RF)-positive sera (RF value of ≤850 IU/ml; n = 11) although a higher concentration of RF in samples might cause cross-reactivity. The new test has a good agreement of 97.2% with a kappa value of 0.943 when compared with a reference enzyme-linked immunosorbent assay. The positive predictive value and the negative predictive value for the rapid test thus reached 98.0 and 97.6%, respectively. This is the first rapid, point-of-care test for hepatitis E and will be especially useful for the diagnosis of acute hepatitis E virus infection in field and emergency settings and in resource-poor countries.

Empty virus-like particle-based enzyme-linked immunosorbent assay for antibodies to hepatitis E virus

2000 ◽  
Vol 62 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Tian-Cheng Li ◽  
Jing Zhang ◽  
Haruhide Shinzawa ◽  
Masamichi Ishibashi ◽  
Michio Sata ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Like Particle

scholarly journals Double-Antigen Enzyme-Linked Immunosorbent Assay for Detection of Hepatitis E Virus-Specific Antibodies in Human or Swine Sera

2008 ◽  
Vol 15 (8) ◽  
pp. 1151-1157 ◽  
Author(s):  
Wei Ping Hu ◽  
Yang Lu ◽  
Nestor Amadeo Precioso ◽  
Hsiao Ying Chen ◽  
Teresa Howard ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Predictive Value ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Specific Antibodies ◽  
Kappa Value ◽  
Healthy Control ◽  
The Individual

ABSTRACT A new double-antigen sandwich-based enzyme-linked immunosorbent assay (ELISA) for the detection of total antibodies (immunoglobulin G [IgG] and IgM) specific for hepatitis E virus (HEV) was developed by utilizing well-characterized recombinant protein ET2.1 and its peroxidase-labeled counterpart. Our study showed that the ELISA detected all the positive patient samples (n = 265) regardless of whether they contained IgM or IgG antibodies, or both, while it maintained an excellent specificity of 98.8% with samples from various patient or healthy control groups (total number of samples, 424). The test had a detection limit for anti-HEV IgG antibodies that was equivalent to 62 mIU/ml of the international reference. Compared with the serological status of the specimens determined on the basis of tests performed at the individual collection sites, the testing outcome generated by the new ELISA had a good agreement of 99.3%, with a kappa value of 0.985. The positive predictive value and the negative predictive value for the new test reached 98.1% and 100%, respectively. This ELISA had a positive delta value of 4.836 and a negative delta value of 3.314 (where delta is a measure of the number of standard deviations by which the cutoff is separated from the mean of the sample groups) (N. Crofts, W. Maskill, and I. D. Gust, J. Virol. Methods 22:51-59, 1988), indicating that it had an excellent ability to differentiate the infected and noninfected cohorts. Furthermore, the new design enables the detection of antibodies not only in human samples but also in pig samples. Our preliminary data showed that the ELISA could detect seroconversion in samples from pigs at as early as 14 days postinoculation. The potential utility of detecting specific antibodies in pigs will be an added advantage for managing the disease, with suggested zoonotic implications.

scholarly journals Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Antibody to Epizootic Hemorrhagic Disease of Deer Virus

2000 ◽  
Vol 12 (2) ◽  
pp. 142-145 ◽  
Author(s):  
James O. Mecham ◽  
Michael M. Jochim
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hemorrhagic Disease ◽  
Structural Protein ◽  
Serum Samples ◽  
Epizootic Hemorrhagic Disease ◽  
Diagnostic Potential ◽  
The Us ◽  
Serotype 1 ◽  
Infected Cells

An enzyme-linked immunosorbent assay has been developed to detect antibodies to epizootic hemorrhagic disease of deer virus (EHDV). The assay incorporates a monoclonal antibody to EHDV serotype 2 (EHDV-2) that demonstrates specificity for the viral structural protein, VP7. The assay was evaluated with sequential sera collected from cattle experimentally infected with EHDV serotype 1 (EHDV-1) and EHDV-2, as well as the four serotypes of bluetongue virus (BTV), BTV-10, BTV-11, BTV-13, and BTV-17, that currently circulate in the US. A competitive and a blocking format as well as the use of antigen produced from both EHDV-1-and EHDV-2-infected cells were evaluated. The assay was able to detect specific antibody as early as 7 days after infection and could differentiate animals experimentally infected with EHDV from those experimentally infected with BTV. The diagnostic potential of this assay was demonstrated with field-collected serum samples from cattle, deer, and buffalo.

scholarly journals DESIGN OF HEPATITIS E VIRUS GENOTYPE 1 RECOMBINANT ORF3 PROTEIN BY CODON OPTIMIZATION METHOD

2017 ◽  
pp. 63-72
Author(s):  
G. I. Alatortseva ◽  
A. V. Sidorov ◽  
L. N. Nesterenko ◽  
L. N. Luhverchik ◽  
M. V. Zhukina ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Hepatitis E Virus ◽  
Optimization Method ◽  
Hepatitis E ◽  
Antigenic Specificity ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Genotype 1 ◽  
Recombinant Polypeptide ◽  
Full Size

Aim. The development of the hepatitis E virus (HEV) genotype 1 full-size ORF3 recombinant polypeptide. Materials and methods. Escherichia coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical and serological methods. Results. HEV genotype 1 RNA had been isolated from clinical samples collected in Kyrgyzstan. DNA copy of subgenomic virus RNA had been cloned and used for further development of E.coli strains producing full-size recombinant protein ORF3 fused to E.coli beta-galactosidase. Codons optimization method was used in aim to increase expression level of recombinant protein. Recombinant protein ORF3 had been isolated from the inclusion bodies of the E.coli biomass and purified by size exclusion chromatography. Antigenic specificity of recombinant polypeptide had been confirmed by enzyme-linked immunosorbent assay and Western blotting with the specific sera. Conclusion. HEVgenotype 1 ORF3 recombinant antigen had been designed, and it’s applicability in diagnostic tests had been experimentally confirmed.

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