scholarly journals Comparison of a New Immunochromatographic Test to Enzyme-Linked Immunosorbent Assay for Rapid Detection of Immunoglobulin M Antibodies to Hepatitis E Virus in Human Sera

2005 ◽  
Vol 12 (5) ◽  
pp. 593-598 ◽  
Author(s):  
Hsiao Ying Chen ◽  
Yang Lu ◽  
Teresa Howard ◽  
David Anderson ◽  
Priscilla Yiquan Fong ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Immunosorbent Assay ◽  
Predictive Value ◽  
Rapid Detection ◽  
Hepatitis E Virus ◽  
Rapid Test ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Acute Hepatitis E

ABSTRACT An immunochromatographic test for rapid detection of IgM antibodies in patients with acute hepatitis E infection was developed utilizing the well-characterized recombinant protein EP2.1 and monoclonal antibody 4B2. The new rapid test based on a novel reverse-flow technology was able to generate a positive result within 2 to 3 min. Our study showed that this test was able to detect anti-HEV IgM antibodies in 96.7% of the patient samples tested (n = 151) while maintaining an excellent specificity of 98.6% with samples from various patient or healthy control groups (total n = 208). Furthermore, this rapid test gave a good specificity of 90.9% when tested with rheumatoid factor (RF)-positive sera (RF value of ≤850 IU/ml; n = 11) although a higher concentration of RF in samples might cause cross-reactivity. The new test has a good agreement of 97.2% with a kappa value of 0.943 when compared with a reference enzyme-linked immunosorbent assay. The positive predictive value and the negative predictive value for the rapid test thus reached 98.0 and 97.6%, respectively. This is the first rapid, point-of-care test for hepatitis E and will be especially useful for the diagnosis of acute hepatitis E virus infection in field and emergency settings and in resource-poor countries.

scholarly journals Good Performance of Immunoglobulin M Assays in Diagnosing Genotype 3 Hepatitis E Virus Infections

2009 ◽  
Vol 16 (5) ◽  
pp. 772-774 ◽  
Author(s):  
Florence Legrand-Abravanel ◽  
Isabelle Thevenet ◽  
Jean-Michel Mansuy ◽  
Karine Saune ◽  
Françoise Vischi ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis E Virus ◽  
Rapid Test ◽  
Hepatitis E ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Genotype 3

ABSTRACT We have evaluated three anti-hepatitis E virus (anti-HEV) immunoglobulin M (IgM) assays, the EIAgen HEV IgM assay (Adaltis), the HEV IgM enzyme-linked immunosorbent assay 3.0, and the Assure HEV IgM rapid test (MP Diagnostics), for the routine detection of acute genotype 3 HEV. Their sensitivities were fairly good (90%, 88%, and 82%), and their specificities were excellent (100%, 99.5%, and 100%).

scholarly journals Double-Antigen Enzyme-Linked Immunosorbent Assay for Detection of Hepatitis E Virus-Specific Antibodies in Human or Swine Sera

2008 ◽  
Vol 15 (8) ◽  
pp. 1151-1157 ◽  
Author(s):  
Wei Ping Hu ◽  
Yang Lu ◽  
Nestor Amadeo Precioso ◽  
Hsiao Ying Chen ◽  
Teresa Howard ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Predictive Value ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Specific Antibodies ◽  
Kappa Value ◽  
Healthy Control ◽  
The Individual

ABSTRACT A new double-antigen sandwich-based enzyme-linked immunosorbent assay (ELISA) for the detection of total antibodies (immunoglobulin G [IgG] and IgM) specific for hepatitis E virus (HEV) was developed by utilizing well-characterized recombinant protein ET2.1 and its peroxidase-labeled counterpart. Our study showed that the ELISA detected all the positive patient samples (n = 265) regardless of whether they contained IgM or IgG antibodies, or both, while it maintained an excellent specificity of 98.8% with samples from various patient or healthy control groups (total number of samples, 424). The test had a detection limit for anti-HEV IgG antibodies that was equivalent to 62 mIU/ml of the international reference. Compared with the serological status of the specimens determined on the basis of tests performed at the individual collection sites, the testing outcome generated by the new ELISA had a good agreement of 99.3%, with a kappa value of 0.985. The positive predictive value and the negative predictive value for the new test reached 98.1% and 100%, respectively. This ELISA had a positive delta value of 4.836 and a negative delta value of 3.314 (where delta is a measure of the number of standard deviations by which the cutoff is separated from the mean of the sample groups) (N. Crofts, W. Maskill, and I. D. Gust, J. Virol. Methods 22:51-59, 1988), indicating that it had an excellent ability to differentiate the infected and noninfected cohorts. Furthermore, the new design enables the detection of antibodies not only in human samples but also in pig samples. Our preliminary data showed that the ELISA could detect seroconversion in samples from pigs at as early as 14 days postinoculation. The potential utility of detecting specific antibodies in pigs will be an added advantage for managing the disease, with suggested zoonotic implications.

scholarly journals Acute hepatitis E virus infection in human immunodeficiency virus-positive men and women in Nepal: Not quite a rare entity

Hepatology ◽  
2016 ◽  
Vol 64 (3) ◽  
pp. 1006-1007 ◽  
Author(s):  
Birendra Prasad Gupta ◽  
Ananta Shrestha ◽  
Anurag Adhikari ◽  
Thupten Kelsang Lama ◽  
Binaya Sapkota
Keyword(s):  
Human Immunodeficiency Virus ◽  
Virus Infection ◽  
Acute Hepatitis ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Rare Entity ◽  
Men And Women ◽  
Immunodeficiency Virus ◽  
Acute Hepatitis E

Evidence for the presence of autochthonous (locally acquired) cases of acute hepatitis E virus infections in Italy since the 80s

2015 ◽  
Vol 26 (5) ◽  
pp. 348-350 ◽  
Author(s):  
Tommaso Stroffolini ◽  
Maria Rapicetta ◽  
Paola Chionne ◽  
Rozenn Esvan ◽  
Elisabetta Madonna ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Infections ◽  
Acute Hepatitis E

scholarly journals A Case of Acute Hepatitis E Infection in a Patient with Non-Hodgkin Lymphoma Treated Successfully with Ribavirin

2017 ◽  
Vol 2017 ◽  
pp. 1-3 ◽  
Author(s):  
Hasan N. Y. Haboubi ◽  
Rizwan Diyar ◽  
Ann Benton ◽  
Chin Lye Ch’ng
Keyword(s):  
Acute Hepatitis ◽  
Hodgkin Lymphoma ◽  
Hepatitis E Virus ◽  
Immunosuppressive Treatment ◽  
Risk Groups ◽  
Pegylated Interferon ◽  
Hepatitis E ◽  
Additional Treatment ◽  
Non Hodgkin Lymphoma ◽  
Acute Hepatitis E

We present the case of a man who, following immunosuppressive treatment for non-Hodgkin lymphoma, became infected with viral hepatitis E. Acute hepatitis E virus infection should be considered in patients with deranged liver function on a background of haematological malignancies or immunosuppression, even without travel to endemic regions. Whilst clearance is usually spontaneous in immune-competent individuals, these at-risk groups may develop a more complicated and protracted disease course. Thus awareness is important as additional treatment with ribavirin or pegylated interferon may be required, as in this case, in order to help achieve eradication.

Three male patients with sporadic acute hepatitis E in Sendai, Japan, who were domestically infected with hepatitis E virus of genotype III or IV

2004 ◽  
Vol 39 (3) ◽  
pp. 292-298 ◽  
Author(s):  
Takeshi Yamamoto ◽  
Hiroshi Suzuki ◽  
Takayoshi Toyota ◽  
Masaharu Takahashi ◽  
Hiroaki Okamoto
Keyword(s):  
Acute Hepatitis ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype Iii ◽  
Sporadic Acute Hepatitis ◽  
Acute Hepatitis E ◽  
Male Patients
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