scholarly journals Genetic and Antigenic Evidence Supports the Separation of Hepatozoon canis and Hepatozoon americanum at the Species Level

2000 ◽  
Vol 38 (3) ◽  
pp. 1298-1301 ◽  
Author(s):  
Gad Baneth ◽  
John R. Barta ◽  
Varda Shkap ◽  
Donald S. Martin ◽  
Douglass K. Macintire ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
18S Rrna ◽  
Distinct Species ◽  
18S Rrna Gene ◽  
Species Level ◽  
Hepatozoon Canis ◽  
Rrna Gene ◽  
Western Immunoblotting ◽  
Hepatozoon Americanum

Recognition of Hepatozoon canis and Hepatozoon americanum as distinct species was supported by the results of Western immunoblotting of canine anti-H. canis and anti-H. americanum sera against H. canisgamonts. Sequence analysis of 368 bases near the 3′ end of the 18S rRNA gene from each species revealed a pairwise difference of 13.59%.

scholarly journals Prevalence and distribution pattern of Cryptosporidium spp. among pre-weaned diarrheic calves in the Republic of Korea

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259824
Author(s):  
Dong-Hun Jang ◽  
Hyung-Chul Cho ◽  
Seung-Uk Shin ◽  
Eun-Mi Kim ◽  
Yu-Jin Park ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Infection Rate ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Republic Of Korea ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
High Genetic Diversity ◽  
The Republic ◽  
Diarrheic Calves

Cryptosporidium spp. are protozoan parasites that belong to subphylum apicomplexa and cause diarrhea in humans and animals worldwide. Data on the prevalence of Cryptosporidium spp. and its subtypes among calves in the Republic of Korea (KOR) are sparse. Hence, our study aimed to investigate the prevalence and association between the age of calf and the identified Cryptosporidium spp. and to determine the genotypes/subtypes of Cryptosporidium spp. in pre-weaned calves with diarrhea in the KOR. A total of 460 diarrheic fecal samples were collected from calves aged 1−60 days and screened for Cryptosporidium spp. by the 18S rRNA gene. Species identification was determined using the sequencing analysis of the 18S rRNA gene, and C. parvum-positive samples were subtyped via the sequence analysis of the 60-kDa glycoprotein (gp60) gene. Sequence analysis based on the 18S rRNA gene revealed the presence of three Cryptosporidium spp., namely, C. parvum (n = 72), C. ryanae (n = 12), and C. bovis (n = 2). Co-infection by these species was not observed. The infection rate was the highest in calves aged 11−20 days (26.1%, 95% CI 17.1−35.1), whereas the lowest rate was observed in calves aged 21−30 days (7.7%, 95% CI 0.0−16.1). The prevalence of C. parvum was detected exclusively in calves aged ≤20 days, and the highest infection rate of C. ryanae was seen in calves ≥31 days of age. The occurrence of C. parvum (χ2 = 25.300, P = 0.000) and C. ryanae (χ2 = 18.020, P = 0.001) was significantly associated with the age of the calves. Eleven different subtypes of the IIa family that belonging to C. parvum were recognized via the sequence analyses of the gp60 gene. Except for two (IIaA18G3R1 and IIaA15G2R1) subtypes, nine subtypes were first identified in calves with diarrhea in the KOR. IIaA18G3R1 was the most frequently detected subtype (72.2% of calves), followed by IIaA17G3R1 (5.6%), IIaA15G2R1 (4.2%), IIaA19G4R1 (4.2%), IIaA16G4R1 (2.8%), IIaA17G4R1 (2.8%), IIaA19G3R (2.8%), IIaA14G1R1 (1.4%), IIaA14G3R1 (1.4%), IIaA15G1R1 (1.4%), and IIaA19G1R1 (1.4%) These results suggest that the prevalence of Cryptosporidium spp. is significantly associated with calf age. Furthermore, the findings demonstrate the high genetic diversity of C. parvum and the widespread occurrence of zoonotic C. parvum in pre-weaned calves. Hence, calves are a potential source of zoonotic transmission with considerable public health implications.

scholarly journals Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of the Maize Mitochondrial Genome

1983 ◽  
Vol 71 (1) ◽  
pp. 190-193 ◽  
Author(s):  
Shiaoman Chao ◽  
Ronald R. Sederoff ◽  
Charles S. Levings
Keyword(s):  
Sequence Analysis ◽  
Mitochondrial Genome ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Gene Region ◽  
Partial Sequence ◽  
Rrna Gene

Molecular characterization of arthropod-borne hematozoans in wild mammals from Brazil, Venezuela and Spain

2009 ◽  
Vol 54 (3) ◽  
Author(s):  
Angel Criado-Fornelio ◽  
Amelia Buling ◽  
Nieves Casado ◽  
Consuelo Gimenez ◽  
Jeronimo Ruas ◽  
...  
Keyword(s):  
Cervus Elaphus ◽  
18S Rrna ◽  
Red Deer ◽  
18S Rrna Gene ◽  
Hepatozoon Canis ◽  
Rrna Gene ◽  
Red Squirrel ◽  
Wild Mammals ◽  
Pine Martens ◽  
Gene Similarity

AbstractA survey of Babesia, Theileria and Hepatozoon was conducted in wild mammals, including the capybara (Hydrochaeris hydrochaeris; n = 14) from Brazil, the jaguar (Panthera onca; n = 2) and crab-eating raccoon (Procyon cancrivorus; n = 4) from Venezuela, and the red deer (Cervus elaphus; n = 70), red squirrel (Sciurus vulgaris; n = 5) and Eurasian pine marten (Martes martes; n = 3) from Spain. Diagnostic procedures included both microscopy and molecular methods (PCR and sequencing of the 18S rRNA gene). Microscopic examination of blood smears revealed no hematozoan infections — unlike the molecular analyses. Nine Brazilian capybaras were found to be infected with Hepatozoon canis (prevalence 64%), two of which were coinfected with a previously unknown babesid (prevalence 14%) loosely related to Theileria equi (90% 18S rRNA gene similarity according to BLAS® analysis). One jaguar and one crab-eating raccoon from Venezuela were infected by H. canis. Four of the red deer were infected with theilerids (5.7% prevalence), two with Theileria sp. and two with T. annulata. One red squirrel and three pine martens were infected with Hepatozoon sp. The isolate form the red squirrel was phylogenetically related to Hepatozoon sp. reported in Spanish bank voles, whereas those infecting the pine martens were related to Hepatozoon felis reported in Spanish cats. In conclusion, the molecular findings show that some non-canid mammals are carriers of H. canis in South America, while red deer may carry T. annulata in Europe. Small mammals in Europe appear to be unlikely hosts of H. canis and H. felis.

scholarly journals DifferentiatingDracunculusmedinensisfromD.insignis, by the sequence analysis of the 18S rRNA gene

2005 ◽  
Vol 99 (5) ◽  
pp. 511-517 ◽  
Author(s):  
L. Bimi ◽  
A. R. Freeman ◽  
M. L. Eberhard ◽  
E. Ruiz-Tiben ◽  
N. J. Pieniazek
Keyword(s):  
Sequence Analysis ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene

Morphology and molecular analysis of the 18S rRNA gene of oyster shell borers, Polydora species (Polychaeta: Spionidae), from Japan and Australia

2013 ◽  
Vol 93 (5) ◽  
pp. 1279-1286 ◽  
Author(s):  
Waka Sato-Okoshi ◽  
Hirokazu Abe
Keyword(s):  
18S Rrna ◽  
Oyster Shell ◽  
First Record ◽  
18S Rrna Gene ◽  
Species Level ◽  
Rrna Gene ◽  
Species Determination ◽  
Wide Range ◽  
First Time ◽  
Black Pigmentation

Nuclear 18S rRNA gene sequences of oyster shell borers, Polydora websteri, P. calcarea and P. haswelli (Polychaeta: Spionidae), were determined for the first time. A wide range of morphological variation, particularly with regard to pigmentation, is observed among these three species. This variation was characterized at the species level. Black pigmentation pattern on the palps is suggested to be a key characteristic for species determination. Polydora websteri and P. haswelli were sister species. This is the first record of P. calcarea from Japan and Australia and P. haswelli from Japan.

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