Development of a Serological Test forHaemophilus ducreyi for Seroprevalence Studies
2000 ◽
Vol 38
(4)
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pp. 1520-1526
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Keyword(s):
We developed a new enzyme immunoassay (rpEIA) for use in determining the seroprevalence of chancroid. Three highly conserved outer membrane proteins from Haemophilus ducreyi strain 35000 were cloned, overexpressed, and purified from Escherichia coli for use as antigens in the rpEIA. Serum specimens from patients with and without chancroid were assayed to determine optimum sensitivity and specificity and to establish cutoff values. On the basis of these data, rpEIA was found to be both sensitive and specific when used to test a variety of serum specimens from patients with genital ulcers and urethritis and from healthy blood donors.
1981 ◽
Vol 113
(2)
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pp. 375-380
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Keyword(s):
1979 ◽
Vol 140
(3)
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pp. 843-847
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Keyword(s):
1978 ◽
Vol 133
(3)
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pp. 1478-1483
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1996 ◽
Vol 19
(6)
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pp. 1287-1294
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1985 ◽
Vol 21
(3)
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pp. 442-444
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1987 ◽
Vol 25
(11)
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pp. 2181-2188
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Keyword(s):
1987 ◽
Vol 169
(9)
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pp. 4327-4334
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Keyword(s):