scholarly journals Detection of Rickettsia prowazekii in Body Lice and Their Feces by Using Monoclonal Antibodies

2002 ◽  
Vol 40 (9) ◽  
pp. 3358-3363 ◽  
Author(s):  
R. Fang ◽  
L. Houhamdi ◽  
D. Raoult
2000 ◽  
Vol 7 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Zhongxing Liang ◽  
Didier Raoult

ABSTRACT Seven species-specific monoclonal antibodies (MAbs) toBartonella quintana were produced and characterized. The MAbs were of the immunoglobulin G class and reacted only with 13B. quintana strains in indirect microimmunofluorescence and Western immunoblotting assays. They did not react with eight otherBartonella spp., including Bartonella henselae, the most closely related species, and a selected MAb did also not react with nine other strains of gram-negative bacteria. The MAbs reacted mainly with a 34-kDa protein epitope of B. quintana which was shown to be species specific by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Four of five body lice experimentally infected with B. quintana were found to be positive for the organism in microimmunofluorescence assays with one MAb. These MAbs may provide a specific, simple, rapid, and low-cost tool for the identification of B. quintana and the diagnosis of infections due to the microorganism.


2021 ◽  
Vol 70 (1) ◽  
pp. 21-29
Author(s):  
Svetlana V. Golikova ◽  

The paper describes sanitary-hygienic, epidemiological, medical and food measures against carried out by medics during the outbreaks of typhus in 1908–1914. As this infection became a hunger marker in Russia, the object of the study is an epidemic situation in three counties (“uezd”) of the Perm province — Ekaterinburgskiy, Kamyshlovskiy, Shadrinskiy — which population constantly suffered from a crop failure. Based on the materials of “The medical and sanitary chronicle of the Perm province”, (“Vrachebno-sanitarnaya hronika Permskoy gubernii”) containing publications describing epidemics of typhus, the author considers application of the achievements of biological and medical sciences for outlining rational strategy for measures against typhus (implementation of disinfection and disinsection procedures) and identifies two organizational innovations introduced in that time. The first epidemiological teams consisting of doctors, paramedics (“fel’dsher”) and nurses were organized and sent to the epidemic focuses. These units were intended to detect, isolate and treat typhus patients, carry out disinfection and disinfestation in special temporary anti-typhoid barracks. The population began to use temporary hospitals actively, disinfect their houses and property. However, the insufficient knowledge of a transmission pathway of infection (its causative agent — Rickettsia prowazekii — and the carrier — body lice) hampered the further development of effective anti-typhoid measures and, as a result, challenged the whole complex of proposed activities.


Author(s):  
James E. Crandall ◽  
Linda C. Hassinger ◽  
Gerald A. Schwarting

Cell surface glycoconjugates are considered to play important roles in cell-cell interactions in the developing central nervous system. We have previously described a group of monoclonal antibodies that recognize defined carbohydrate epitopes and reveal unique temporal and spatial patterns of immunoreactivity in the developing main and accessory olfactory systems in rats. Antibody CC2 reacts with complex α-galactosyl and α-fucosyl glycoproteins and glycolipids. Antibody CC1 reacts with terminal N-acetyl galactosamine residues of globoside-like glycolipids. Antibody 1B2 reacts with β-galactosyl glycolipids and glycoproteins. Our light microscopic data suggest that these antigens may be located on the surfaces of axons of the vomeronasal and olfactory nerves as well as on some of their target neurons in the main and accessory olfactory bulbs.


Author(s):  
K.S. Kosik ◽  
L.K. Duffy ◽  
S. Bakalis ◽  
C. Abraham ◽  
D.J. Selkoe

The major structural lesions of the human brain during aging and in Alzheimer disease (AD) are the neurofibrillary tangles (NFT) and the senile (neuritic) plaque. Although these fibrous alterations have been recognized by light microscopists for almost a century, detailed biochemical and morphological analysis of the lesions has been undertaken only recently. Because the intraneuronal deposits in the NFT and the plaque neurites and the extraneuronal amyloid cores of the plaques have a filamentous ultrastructure, the neuronal cytoskeleton has played a prominent role in most pathogenetic hypotheses.The approach of our laboratory toward elucidating the origin of plaques and tangles in AD has been two-fold: the use of analytical protein chemistry to purify and then characterize the pathological fibers comprising the tangles and plaques, and the use of certain monoclonal antibodies to neuronal cytoskeletal proteins that, despite high specificity, cross-react with NFT and thus implicate epitopes of these proteins as constituents of the tangles.


1996 ◽  
Vol 26 (10) ◽  
pp. 1182-1187 ◽  
Author(s):  
P. RESTANI ◽  
A. PLEBANI ◽  
T. VELONA ◽  
G. CAVAGNI ◽  
A. G. UGAZIO ◽  
...  

Ob Gyn News ◽  
2008 ◽  
Vol 43 (4) ◽  
pp. 12 ◽  
Author(s):  
GERALD G. BRIGGS

Sign in / Sign up

Export Citation Format

Share Document