Rapid carbohydrate fermentation test for confirmation of the pathogenic Neisseria using a Ba(OH)2 indicator

1977 ◽  
Vol 5 (1) ◽  
pp. 15-19
Author(s):  
M Slifkin ◽  
G R Pouchet
Keyword(s):  
Indicator System ◽  
Rapid Identification ◽  
Clinical Specimens ◽  
Practical Procedure ◽  
Carbohydrate Fermentation ◽  
Fermentation Test

The Ba(OH)2 indicator system was demonstrated to be a practical procedure in assisting clinical bacteriologists in the accurate and rapid identification of the pathogenic Neisseria from clinical specimens. This system measured the release of CO2, resulting from the metabolism of fermentable carbohydrate, as the precipitated BaCO3, by means of a spectrophotometer, The method was uncomplicated and can be performed in most clinical bacteriology laboratories.

scholarly journals Comparison of diagnostic clinical samples and environmental sampling for enterovirus and parechovirus surveillance in Scotland, 2010 to 2012

2014 ◽  
Vol 19 (15) ◽  
Author(s):  
H Harvala ◽  
J Calvert ◽  
D Van Nguyen ◽  
L Clasper ◽  
N Gadsby ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Rapid Identification ◽  
Clinical Samples ◽  
Environmental Sampling ◽  
Clinical Specimens ◽  
Young Infants ◽  
Coxsackievirus A6 ◽  
The Family ◽  
Common Causes

Human enteroviruses (EV) and parechoviruses (HPeV) within the family Picornaviridae are the most common causes of viral central nervous system (CNS)-associated infections including meningitis and neonatal sepsis-like disease. The frequencies of EV and HPeV types identified in clinical specimens collected in Scotland over an eight-year period were compared to those identified in sewage surveillance established in Edinburgh. Of the 35 different EV types belonging to four EV species (A to D) and the four HPeV types detected in this study, HPeV3 was identified as the most prevalent picornavirus in cerebrospinal fluid samples, followed by species B EV. Interestingly, over half of EV and all HPeV CNS-associated infections were observed in young infants (younger than three months). Detection of species A EV including coxsackievirus A6 and EV71 in clinical samples and sewage indicates that these viruses are already widely circulating in Scotland. Furthermore, species C EV were frequently identified EV in sewage screening but they were not present in any of 606 EV-positive clinical samples studied, indicating their likely lower pathogenicity. Picornavirus surveillance is important not only for monitoring the changing epidemiology of these infections but also for the rapid identification of spread of emerging EV and/or HPeV types.

scholarly journals A simple carbohydrate fermentation test for identification of the pathogenic Neisseria

1975 ◽  
Vol 2 (1) ◽  
pp. 72-73
Author(s):  
A Reddick
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Mueller Hinton ◽  
Paper Disk ◽  
Carbohydrate Fermentation ◽  
Fermentation Test ◽  
Agar Method ◽  
Disk Method ◽  
Mueller Hinton Broth ◽  
Simple Carbohydrate

The carbohydrate fermentation test in cystine-Trypticase agar-tubed medium was compared with the Minitek system with carbohydrate-impregnated paper disks in Müeller-Hinton broth for identification of Neisseria gonorrhoeae and N. meningitidis. There was 100% agreement between the methods for confirmation of N. meningitidis. The paper disk method confirmed 98% of the N. gonorrhoeae isolates; the cystine-Trypticase agar method confirmed 96%. Reactions with the paper disk method could be read in 4 h.

scholarly journals Detection of Nontuberculous Mycobacterium by Real Time PCR from Variety of Clinical Specimens

Pulse ◽  
2017 ◽  
Vol 9 (1) ◽  
pp. 15-21
Author(s):  
MM Rahman ◽  
R Rahim ◽  
F Nasrin ◽  
AH Rasel ◽  
A Khaled ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Sequences ◽  
Real Time Pcr ◽  
Respiratory Infections ◽  
Multidrug Resistant ◽  
Rapid Identification ◽  
Nontuberculous Mycobacterium ◽  
Specific Primers ◽  
Clinical Specimens ◽  
Mdr Tb

Aim: Nontuberculous mycobacterium (NTM) causes many types of infections including respiratory and non-respiratory infections such as skin and soft tissue infections, lymphadenitis, meningitis, gastro-intestinal infections, disseminated infections and even intravenous catheter-related infections. Increasing incidence of NTM is reported worldwide in last decade. However, incidence of NTM in Bangladesh is not known as detection of NTM is not undergoing in Bangladesh which is necessary to know as these NTM species are resistant to first-line anti-TB drugs and, when mistaken for M. tuberculosis, give rise to erroneous identification of multidrug-resistant TB (MDR-TB). We wanted to know the existence of NTM from various clinical specimens including tissues from tuberculosis suspected patients visited in Apollo Hospitals Dhaka in 2013 to 2015.Material and Method: Sample processing, DNA extraction and real time PCR (polymerase chain reaction) were done according to the commercial LyteStar TB/NTM PCR kit developed by Altona Diagnostics, Germany. The target DNA sequences are amplified with IS61 10-specific primers for MTB complex and ITS-specific primers for NTM. Probes specific for MTB complex and NTM DNA are labeled with fluorophore dye FAM and HEX, respectively. We have analyzed 579 clinical specimens from tuberculosis suspected patient.Result: Among 579 specimen different types of tissues were 201 and histopathology data were available for 166 cases. In tissues NTM was detected by PCR in 3 1(19%) cases, 8 of which were compatible with histopathology findings and rest 23 cases showed no evidence of granulomatous lesion. We analyzed 378 different varieties of clinical specimens such as sputum, bronchial lavages, body fluids, pus and swabs. Among 378 samples 215 samples were requested for AFB staining. NTM was detected by PCR in 19(8.8%) samples and out of 19 NTM positive specimens only one was AFB positive.Conclusion: This is the first report in the country about detection of NTM in variety of clinical specimens and warrants further elaborate investigation. Our results showed that PCR is an effective tool for the rapid identification of NTM from tissues and AFB negative clinical specimens having suspicion for mycobacterial infection.Pulse Vol.9 January-December 2016 p.15-21

A new species of marine yeast Kluyveromyces penaeid isolated from the heart of penaeid shrimp Penaeus chinensis

1999 ◽  
Vol 79 (3) ◽  
pp. 559-561 ◽  
Author(s):  
Shang-Liang Tong ◽  
Hong-Zhi Miao
Keyword(s):  
New Species ◽  
Penaeid Shrimp ◽  
Heart Tissue ◽  
Urea Hydrolysis ◽  
Marine Yeast ◽  
Penaeus Chinensis ◽  
Defined Media ◽  
Carbohydrate Fermentation ◽  
Fermentation Test ◽  
A New Species

A new species of marine yeast Kluyveromyces penaeid (Saccharomycetoideae) was isolated from the heart tissue of a subadult shrimp Penaeus chinensis during tissue culture. The yeast grew well in seawater supplemented with 2% shrimp extract, but did not grow in chemically defined media. The vegetative cells reproduced by multilateral budding and formed rudimentary pseudohyphae occasionally. Asci were spheroidal and evanescent containing 2–13 smooth or oval ascospores. The best temperature for the yeast to grow was 20–25°C and 37°C was lethal. The yeast grew well in half to full strength seawater supplemented with shrimp extract, but did not grow in 25% strength seawater. The carbohydrate fermentation test was positive, the diazonium blue B and urea hydrolysis tests were negative.

scholarly journals Characterization and identification of 95 diphtheroid (group JK) cultures isolated from clinical specimens

1979 ◽  
Vol 9 (3) ◽  
pp. 418-424 ◽  
Author(s):  
P S Riley ◽  
D G Hollis ◽  
G B Utter ◽  
R E Weaver ◽  
C N Baker
Keyword(s):  
Liquid Chromatography ◽  
Antimicrobial Agents ◽  
Blood Cultures ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Clinical Specimens ◽  
Bacterial Cultures ◽  
Carbohydrate Fermentation ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

Ninety-five cultures of group JK bacteria isolated from clinical specimens were characterized morphologically and biochemically. The microorganisms were isolated primarily from blood cultures. The bacterial cultures produced positive reactions when tested for catalase, Tween hydrolysis, and carbohydrate fermentation. Glucose and galactose were fermented by more than 90% of the organisms. Gas-liquid chromatography of trimethylsilyl derivatives of whole-cell hydrolysates of some of the group JK cultures yielded nearly identical elution profiles. The group JK microorganisms were susceptible to vancomycin but were resistant to most of the other 17 antimicrobial agents tested. A method is presented for differentiating the group JK microorganisms from other similar bacteria encountered in clinical specimens. Although these bacteria rarely occur in clinical specimens, they are capable of producing fatal infections (endocarditis and sepsis) in humans.

Rapid identification of Mycobacterium species isolated from clinical specimens of city Jahrom by real time PCR

Gene Reports ◽  
2020 ◽  
Vol 21 ◽  
pp. 100847
Author(s):  
Hadi Rezaii Yazdi ◽  
Mojtaba Azadbakht ◽  
Hossein Foruozandeh ◽  
Masoud Torabi Ardakani ◽  
Elmira Zarei
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Identification ◽  
Mycobacterium Species ◽  
Clinical Specimens
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