New method for the isolation of Streptococcus mutans and its differentiation from other oral streptococci

1977 ◽  
Vol 5 (6) ◽  
pp. 604-609
Author(s):  
H A Linke
Keyword(s):  
Staphylococcus Aureus ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Agar Medium ◽  
Etiological Agent ◽  
Oral Streptococci ◽  
Anaerobic Conditions ◽  
Unique Composition ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

A new, improved agar medium for the isolation of Streptococcus mutans, the etiological agent of dental caries, was developed. In contrast to mitis-salivarius agar, this medium not only recovers a greater number of S. mutans strains from most oral specimens but, because of its mannitol and sorbitol content, it also facilitates the differentiation of S. mutans from other oral streptococci, e.g., S. salivarius, S. mitis, and S. sanguis, which do not grow or produce scanty growth only after 10 days of incubation. The medium is easy to prepare because of its simple and unique composition, is characterized by the presence of an acid indicator, and can be utilized under aerobic and anaerobic conditions as well. The medium cannot be used to distinguish among the eight serotypes, a to g and SL-1, of S. mutans. Mannitol-utilizing bacteria such as streptococci (e.g., S. faecalis) and other microorganisms (e.g., Staphylococcus aureus) are able to grow on this medium and can be distinguished from S. mutans by their unique colony morphology.

Performance of Response Surface and Davey Model for Prediction of Staphylococcus aureus Growth Parameters under Different Experimental Conditions

2004 ◽  
Vol 67 (6) ◽  
pp. 1138-1145 ◽  
Author(s):  
G. ZURERA-COSANO ◽  
A. M. CASTILLEJO-RODRÍGUEZ ◽  
R. M. GARCÍA-GIMENO ◽  
F. RINCÓN-LEÓN
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Rate ◽  
Response Surface ◽  
Growth Parameters ◽  
Growth Models ◽  
Lag Time ◽  
Anaerobic Conditions ◽  
Experimental Conditions ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

The combined effect of different temperatures (7 to 19°C), pH levels (4.5 to 8.5), sodium chloride levels (0 to 8%), and sodium nitrite levels (0 to 200 ppm) on the predicted growth rate and lag time of Staphylococcus aureus under aerobic and anaerobic conditions was studied. The two predictive models developed, response surface (RS) and the Davey model, provided reliable estimates of the two kinetic parameters studied. The RS provided better predictions of maximum specific growth rate, with bias factors of 1.06 and 1.31 and accuracy factors of 1.17 and 1.37, respectively, in aerobic and anaerobic conditions. The Davey model performed more accurately for lag time, with a bias factor of 1.12 and an accuracy factor of 1.49, for both aerobic and anaerobic conditions. Predictive growth models are a valuable tool, enabling swift determination of Staphylococcus aureus growth rate and lag time. These data are essential for ensuring staphylococcus-relatedquality and safety of food products.

scholarly journals Comparison of Linezolid Activities under Aerobic and Anaerobic Conditions against Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Enterococcus faecium

2003 ◽  
Vol 47 (1) ◽  
pp. 398-399 ◽  
Author(s):  
Brent W. Gunderson ◽  
Khalid H. Ibrahim ◽  
Charles A. Peloquin ◽  
Laurie B. Hovde ◽  
John C. Rotschafer
Keyword(s):  
Staphylococcus Aureus ◽  
Enterococcus Faecium ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Anaerobic Conditions ◽  
Methicillin Resistant ◽  
Vancomycin Resistant ◽  
Model Drug ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

ABSTRACT Methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium were exposed to linezolid (MIC of 2 mg/liter) under aerobic or anaerobic conditions in an in vitro pharmacodynamic model. Drug concentration and half-life were adjusted to simulate clinical dosing (600 mg twice daily) of linezolid. Linezolid produced a 2-log10 killing at 24 h, and rates of killing against each of these facultative organisms as measured by mean survival time appeared similar under aerobic and anaerobic conditions.

scholarly journals Bacterial and mycotic flora of the oral cavity in patients using a postresection prosthesis

2002 ◽  
pp. 57-65
Author(s):  
Ljubisa Dzambas ◽  
Ljiljana Suvajdzic ◽  
Slobodanka Hrvacanin
Keyword(s):  
Oral Cavity ◽  
Correlation Coefficient ◽  
Oral Streptococci ◽  
Quantitative Structure ◽  
Anaerobic Conditions ◽  
Qualitative And Quantitative ◽  
Range Correlation ◽  
The Face ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

The surgeries in the middle third of the face that distort the anatomic and topographic integrity of the oral cavity, the nose and the sinuses, besides functional damage to these regions, also induce changes in their microfloras of diverse hypothetical combinations. This was the reason to start an investigation on the qualitative and quantitative structure of the microfloras of these regions and their changes. The investigation included 35 patients using a postresection prosthesis. The material for bacteriologic and microbiologic analyses was obtained by single swab sampling from six different localizations, cultured on the blood, TKV, ECV, TYCS, SABOURAND and ENDO agar over 24-48 hours, in aerobic and anaerobic conditions. Species belonging to the pyogenic cocci family, enterobacteria, oral streptococci and fungal microflora were detected in both the oral and the postresection cavity. Frequency distribution and Spearman's range correlation coefficient (R = 0.961; SS = 17; 14.280) (p<0.01) reveal the microfloras of the two cavities were almost identical regarding the species/families of the isolated microorganisms.

Metabolic profiling of Staphylococcus aureus cultivated under aerobic and anaerobic conditions with 1H NMR-based nontargeted analysis

2012 ◽  
Vol 58 (6) ◽  
pp. 709-718 ◽  
Author(s):  
Ji-Lu Sun ◽  
Shao-Kang Zhang ◽  
Jing-Yu Chen ◽  
Bei-Zhong Han
Keyword(s):  
Staphylococcus Aureus ◽  
Limiting Factors ◽  
Cell Physiology ◽  
Resonance Spectroscopy ◽  
Metabolic Profiles ◽  
Anaerobic Conditions ◽  
Isoleucine Leucine ◽  
Components Analysis ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Staphylococcus aureus is a major pathogen in the medical area and food-producing sector. Detailed analyses of its basic cell physiology will help comprehensively understand this pathogen, which will be useful for developing novel diagnostic and treatment tools. Oxygen is one of the most crucial growth-limiting factors for S. aureus. In this study, to characterize and distinguish metabolic profiles of S. aureus cultivated under aerobic and anaerobic conditions, nontargeted analyses of both types of cultures were carried out using 1H nuclear magnetic resonance spectroscopy. Fifty compounds were identified by Chenomx software. Characteristics of metabolic profiles were achieved by using principal components analysis. During aerobic growth, S. aureus mainly consumed glucose, alanine, arginine, glycine, isoleucine, leucine, phenylalanine, and acetate. Meanwhile, it accumulated 17 metabolites, mainly 2-oxoglutarate, isobutyrate, isovalerate, succinate, and ethanol. Under anaerobic condition, S. aureus mainly consumed glucose, arginine, and threonine. Meanwhile, it accumulated 13 metabolites, mainly ethanol, lactate, and ornithine. The representative metabolites that could most significantly differentiate metabolic profiles of S. aureus were isobutyrate, isovalerate, and succinate in aerobic cultivation; and lactate, ethanol, and ornithine in anaerobic cultivation. Among these metabolites, isobutyrate and ornithine were present only in aerobic and anaerobic culture, respectively.

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