scholarly journals Isolation of Legionella pneumophila from a transtracheal aspirate

1979 ◽  
Vol 9 (3) ◽  
pp. 457-458
Author(s):  
P H Edelstein ◽  
S M Finegold
Keyword(s):  
In Vitro Culture ◽  
Legionella Pneumophila ◽  
Body Fluids ◽  
Culture Methods

Isolation of Legionella pneumophila from a transtracheal aspirate was achieved by using simple in vitro culture methods. Clinical microbiologists should routinely culture for this organism from appropriate body fluids obtained from normally sterile areas.

scholarly journals Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Erma Prihastanti ◽  
ENDAH D. HASTUTI ◽  
SRI W.A. SUEDY
Keyword(s):  
In Vitro Culture ◽  
Shoot Growth ◽  
Short Communication ◽  
Stem Explants ◽  
Citrus Reticulata ◽  
Culture Methods ◽  
Treatment Groups ◽  
Physiological Conditions ◽  
Growth Differences

Abstract. Prihastanti E, Hastuti ED, Suedy SWA. 2020. Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods. Biodiversitas 21: 5845-5849. Several efforts have been made to preserve Citrus reticulata var. Tawangmangu and Citrus reticulata var. Garut as indigenous Indonesian mandarin cultivars, including in vitro tissue culture methods. This study aimed to determine growth differences of the stem explants of C. reticulata var. Tawangmangu and C. reticulata var. Garut,which planted on the same Murashige and Skoog (MS) media. The treatment groups were derived from different explants, grown in 4 separate culture bottles for 35 days at 25°C. The observed parameters included the percentage of explants indicating callus development browned-colored explants, and the contaminated explants. Among C. reticulata var. Tawangmangu explants, 23.53% indicated callus development, 29.42% were browned-colored explants, and 0% indicated contamination. In contrast, among the C. reticulata var. Garut explants, 0% indicated callus development, 7.14% brown-colored, and 7.14% indicated contamination. The stems explants from C. reticulata var. Tawangmangu showed a tendency to develop calluses, but the explants of C. reticulata var. Garut was able to support the growth of shoots. C. reticulata var. Tawangmangu and Garut mandarin stem explants showed differences of shoot growth because physiological conditions varied according to the variety.

In vitro culture methods in sorghum with shoot tip as the explant material

2006 ◽  
Vol 25 (3) ◽  
pp. 174-182 ◽  
Author(s):  
N Kishore Sai ◽  
KBRS Visarada ◽  
Y. Aravinda Lakshmi ◽  
E. Pashupatinath ◽  
S. V. Rao ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Shoot Tip ◽  
Culture Methods

Regulation of meiosis in the foetal mouse gonad

Development ◽  
1982 ◽  
Vol 68 (1) ◽  
pp. 59-67
Author(s):  
Clive W. Evans ◽  
Diana I. Robb ◽  
Fiona Tuckett ◽  
Susan Challoner
Keyword(s):  
In Vitro Culture ◽  
Cross Section ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Inhibitory Effects ◽  
Culture Methods ◽  
Male And Female ◽  
Mixed Age ◽  
Gonadal Structure

In vitro culture of male and female gonads was found to have significant effects on gonadal structure and development. Culture resulted in a reduction of testicular cord diameter and a reduction in the number of Sertoli cells lining each cord in cross section. In the female, culture increased the percentage of pyknotic oocytes and fewer germ cells per unit of ovary volume reached diplotene. Mixed sex co-culture using different culture methods showed that day 14 p.c. testes inhibited meiosis in day 14 p.c. ovaries when the cultures were continued until the equivalent of day 21 p.c. Day 15 p.c. and mixed age co-cultures of mixed sex provided more equivocal data since meiosis was inhibited in some preparations but not in others. The possibility is suggested that prophase I may proceed irrevocably to diplotene after about day 15 p.c. and thus the inhibitory effects of foetal testes may be a function of female gonadal age. No evidence was found to support the hypothesis that mixed sex co-culture may stimulate meiosis precociously in foetal testes.

scholarly journals 2230. Analysis of the Microbiological Data from the Delafloxacin (DLX) Phase 3 Community-acquired Bacterial Pneumonia (CABP) Trial

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S761-S762 ◽  
Author(s):  
Sandra McCurdy ◽  
Kara Keedy ◽  
Laura Lawrence ◽  
Amanda Sheets ◽  
Megan Quintas
Keyword(s):  
Legionella Pneumophila ◽  
Chlamydia Pneumoniae ◽  
Oral Treatment ◽  
Phenotypic Characterization ◽  
Clinical Activity ◽  
Microbiological Analysis ◽  
Culture Methods ◽  
Phase 3 ◽  
Atypical Pathogens

Abstract Background DLX is a novel fluoroquinolone (FQ) antibiotic with Gram-positive/MRSA, Gram-negative and atypical activity. It offers IV and oral treatment with no QT restrictions. In a Phase 3 study in CABP patients, DLX was non-inferior to moxifloxacin (MOX) in the primary endpoint, early clinical response at 96 ± 24 hours (88.9 vs. 89.0; 95% CI: −4.4, 4.1) in the intent-to-treat (ITT) population. A detailed microbiological analysis was conducted. Methods CABP pathogens were identified by culture/non-culture methods. Pathogens identified by non-culture methods included Streptococcus pneumoniae (Sp; culture, urinary antigen [UA], nasopharyngeal [NP] swab lytA PCR), Legionella pneumophila (Lp) (culture, UA, serology), Mycoplasma pneumoniae (Mp; culture, serology), and Chlamydia pneumoniae (Cp; serology). All other pathogens were identified using culture only. For Sp cultured from NP, a concomitant lytA PCR value of ≥ 1000 gene copies/mL was required. All isolates underwent susceptibility testing, and a subset of isolates underwent molecular or phenotypic characterization including whole-genome sequencing for FQ resistance mechanisms, PCR for PVL/mecA genes (S. aureus), β-lactamases (Haemophilus/Moraxella spp), and serotyping (Sp). Results Included in submitted image. Conclusion DLX demonstrated potent in vitro and clinical activity against CABP pathogens, including Sp [MRSP, MDRSP, PRSP], MRSA, Haemophilus species, Enterobacteriaceae, P. aeruginosa, and the atypical pathogens Cp, Mp, and Lp. Disclosures All authors: No reported disclosures.

Sign in / Sign up

Export Citation Format

Share Document