scholarly journals Increase of Regeneration Efficiency in JATROPHA CURCAS by In Vitro Culture Methods

2016 ◽  
Author(s):  
Ying LIU ◽  
Jian-Nong LU ◽  
Su-Fang NIU ◽  
Hong-Bo ZHU ◽  
Lin-Feng LI ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Jatropha Curcas ◽  
Culture Methods ◽  
Regeneration Efficiency

scholarly journals Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Erma Prihastanti ◽  
ENDAH D. HASTUTI ◽  
SRI W.A. SUEDY
Keyword(s):  
In Vitro Culture ◽  
Shoot Growth ◽  
Short Communication ◽  
Stem Explants ◽  
Citrus Reticulata ◽  
Culture Methods ◽  
Treatment Groups ◽  
Physiological Conditions ◽  
Growth Differences

Abstract. Prihastanti E, Hastuti ED, Suedy SWA. 2020. Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods. Biodiversitas 21: 5845-5849. Several efforts have been made to preserve Citrus reticulata var. Tawangmangu and Citrus reticulata var. Garut as indigenous Indonesian mandarin cultivars, including in vitro tissue culture methods. This study aimed to determine growth differences of the stem explants of C. reticulata var. Tawangmangu and C. reticulata var. Garut,which planted on the same Murashige and Skoog (MS) media. The treatment groups were derived from different explants, grown in 4 separate culture bottles for 35 days at 25°C. The observed parameters included the percentage of explants indicating callus development browned-colored explants, and the contaminated explants. Among C. reticulata var. Tawangmangu explants, 23.53% indicated callus development, 29.42% were browned-colored explants, and 0% indicated contamination. In contrast, among the C. reticulata var. Garut explants, 0% indicated callus development, 7.14% brown-colored, and 7.14% indicated contamination. The stems explants from C. reticulata var. Tawangmangu showed a tendency to develop calluses, but the explants of C. reticulata var. Garut was able to support the growth of shoots. C. reticulata var. Tawangmangu and Garut mandarin stem explants showed differences of shoot growth because physiological conditions varied according to the variety.

In vitro culture methods in sorghum with shoot tip as the explant material

2006 ◽  
Vol 25 (3) ◽  
pp. 174-182 ◽  
Author(s):  
N Kishore Sai ◽  
KBRS Visarada ◽  
Y. Aravinda Lakshmi ◽  
E. Pashupatinath ◽  
S. V. Rao ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Shoot Tip ◽  
Culture Methods

Regulation of meiosis in the foetal mouse gonad

Development ◽  
1982 ◽  
Vol 68 (1) ◽  
pp. 59-67
Author(s):  
Clive W. Evans ◽  
Diana I. Robb ◽  
Fiona Tuckett ◽  
Susan Challoner
Keyword(s):  
In Vitro Culture ◽  
Cross Section ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Inhibitory Effects ◽  
Culture Methods ◽  
Male And Female ◽  
Mixed Age ◽  
Gonadal Structure

In vitro culture of male and female gonads was found to have significant effects on gonadal structure and development. Culture resulted in a reduction of testicular cord diameter and a reduction in the number of Sertoli cells lining each cord in cross section. In the female, culture increased the percentage of pyknotic oocytes and fewer germ cells per unit of ovary volume reached diplotene. Mixed sex co-culture using different culture methods showed that day 14 p.c. testes inhibited meiosis in day 14 p.c. ovaries when the cultures were continued until the equivalent of day 21 p.c. Day 15 p.c. and mixed age co-cultures of mixed sex provided more equivocal data since meiosis was inhibited in some preparations but not in others. The possibility is suggested that prophase I may proceed irrevocably to diplotene after about day 15 p.c. and thus the inhibitory effects of foetal testes may be a function of female gonadal age. No evidence was found to support the hypothesis that mixed sex co-culture may stimulate meiosis precociously in foetal testes.

scholarly journals Efficiency of Cryoprotectants for In Vitro and Ex Vitro Germination of Cryopreserved Jatropha curcas Seeds

HortScience ◽  
2017 ◽  
Vol 52 (8) ◽  
pp. 1117-1121
Author(s):  
Alexandre Bosco de Oliveira ◽  
Wagner A. Vendrame ◽  
Luciana Cardoso Nogueira Londe
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
Jatropha Curcas ◽  
Germination Percentage ◽  
Seedling Development ◽  
Organic Substrate ◽  
Ex Vitro ◽  
Vitro Experiment ◽  
Jatropha Seeds

To investigate the effects of different cryoprotectants on germination and seedling development of jatropha (Jatropha curcas L.) seeds after cryopreservation, two experiments were performed under in vitro and ex vitro conditions. Nine treatments were used for both experiments, as follows: T1—No cryoprotectants (control); T2—glycerol 2 m (20 minutes); T3—sucrose 0.4 m (20 minutes); T4—glycerol 2 m (20 minutes) + PVS2 (10 minutes); T5—glycerol 2 m (20 minutes) + PVS2 + phloroglucinol 1% (10 minutes); T6—sucrose 0.4 m (20 minutes) + PVS2 (10 minutes); T7—sucrose 0.4 m (20 minutes) + PVS2 + phloroglucinol 1% (10 minutes); T8—glycerol 2 m (20 minutes) + sucrose 0.4 m (20 minutes) + PVS2 (10 minutes); and T9—glycerol 2 m (20 minutes) + sucrose 0.4 m (20 minutes) + PVS2 (10 minutes) + phloroglucinol 1% (10 minutes). After cryopreservation, seeds without cryoprotectants (T1) or with sucrose 0.4 m + PVS2 (T6) returned the best germination percentages after seven days of in vitro culture, 29.5% and 25%, respectively. However, they were not significantly different. For the ex vitro experiment, seed germination percentage was higher in organic substrate. These results indicate that cryopreservation of jatropha seeds can be accomplished without cryoprotectants, and faster germination is obtained in organic substrate.

scholarly journals The use of in vitro culture to improve the propagation of Rhododendron ponticum subsp. baeticum (Boiss. & Reuter)

2007 ◽  
Vol 2 (2) ◽  
pp. 297-306 ◽  
Author(s):  
Manuel Cantos ◽  
Juana Linán ◽  
José García ◽  
María García-Linán ◽  
Miguel Domínguez ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Callus Formation ◽  
Good Method ◽  
The Self ◽  
In Vitro Growth ◽  
Culture Methods ◽  
Rhododendron Ponticum ◽  
Relict Populations

AbstractRhododendron ponticum subsp. baeticum is endemic in the southern region of the Iberian Peninsula. The relict populations of this species are vulnerable, due mainly to difficult conditions for the establishment of seedlings, resulting in a virtual lack of sexual recruitment. In order to preserve the surviving populations, in vitro culture methods have been applied for both the sexual and the agamic propagation of the species. The in vitro germination of seeds was high when conducted with Anderson’s medium without plant growth regulators. The self-rooted seedlings obtained were easily transplanted to outside conditions. The presence of growth regulators in the medium interfered with the development of the seedlings, causing heavy callus formation. The in vitro growth of explants took place readily in Anderson’s medium plus 0.072 mg L−1 of BA and 0.036 mg L−1 of NAA although the explants did not form roots. Rooting was achieved by the basal dipping of the explants in hydroalcoholic solutions of 500 mg L−1 IAA during the outside transplanting process. Therefore, the combination of in vitro grown explants together with ex vitro rooting, results in a good method for the agamic propagation of Rhododendron ponticum subsp. baeticum.

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