Presence of atrs-Like Motif Promotes Rep-Mediated Wild-Type Adeno-Associated Virus Type 2 Integration
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High-throughput integration site (IS) analysis of wild-type adeno-associated virus type 2 (wtAAV2) in human dermal fibroblasts (HDFs) and HeLa cells revealed that juxtaposition of a Rep binding site (RBS) and terminal resolution site (trs)-like motif leads to a 4-fold-increased probability of wtAAV integration. Electrophoretic mobility shift assays (EMSAs) confirmed binding of Rep to off-target RBSs. For the first time, we show Rep protein off-target nicking activity, highlighting the importance of the nicking substrate for Rep-mediated integration.
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2004 ◽
Vol 78
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pp. 6344-6359
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1997 ◽
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pp. 7361-7371
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2001 ◽
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pp. 9991-9994
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2000 ◽
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pp. 9090-9098
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2002 ◽
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pp. 3639-3652
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2000 ◽
Vol 74
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pp. 11511-11521
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