Epstein-Barr virus latency in blood mononuclear cells: analysis of viral gene transcription during primary infection and in the carrier state.

1994 ◽  
Vol 68 (11) ◽  
pp. 7374-7385 ◽  
Author(s):  
R J Tierney ◽  
N Steven ◽  
L S Young ◽  
A B Rickinson
Keyword(s):  
Primary Infection ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Carrier State ◽  
Viral Gene ◽  
Viral Gene Transcription ◽  
Barr Virus ◽  
Virus Latency ◽  
Blood Mononuclear Cells ◽  
Epstein Barr

Epstein–Barr virus

2020 ◽  
pp. 754-763
Author(s):  
Alan B. Rickinson ◽  
M.A. Epstein
Keyword(s):  
Multiple Sclerosis ◽  
Primary Infection ◽  
Epstein Barr Virus ◽  
Human Herpesvirus ◽  
Carrier State ◽  
Previous Exposure ◽  
Barr Virus ◽  
Epidemiologic Evidence ◽  
Double Stranded Dna ◽  
Epstein Barr

Epstein–Barr virus is a human herpesvirus with a linear double-stranded DNA genome that is carried asymptomatically by most people. Symptomless primary infection is usual in childhood, establishing a lifelong carrier state where the virus persists as a latent infection of circulating B cells. The virus replicates recurrently in oropharyngeal epithelial cells, with consequent shedding of virus in saliva transmitting infection. Controversially, Epstein–Barr virus has been linked with certain autoimmune diseases. In particular, there is strong serologic and epidemiologic evidence to suggest that previous exposure to Epstein–Barr virus markedly increases the risk of developing multiple sclerosis. Although the Epstein–Barr virus/multiple sclerosis connection is receiving much attention, the mechanism that might underpin such an association remains uncertain.

scholarly journals In vitro effects of Epstein-Barr virus on peripheral blood mononuclear cells from patients with rheumatoid arthritis and normal subjects.

1978 ◽  
Vol 148 (5) ◽  
pp. 1429-1434 ◽  
Author(s):  
L Slaughter ◽  
D A Carson ◽  
F C Jensen ◽  
T L Holbrook ◽  
J H Vaughan
Keyword(s):  
Rheumatoid Arthritis ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Barr Virus ◽  
Blood Mononuclear Cells ◽  
Epstein Barr

Peripheral blood mononuclear cells from 10 patients with rheumatoid arthritis and 9 control subjects were cultured in vitro for 30 days with and without infection by Epstein-Barr virus. All cultures showed polyclonal stimulation of B cells as indicated by rising levels of IgM in the culture supernates, reaching maximal at 18-24 days, and with no quantitative or kinetic difference between the RA and control cells. IgM anti-IgG was also produced in both groups and maximally at 18-24 days, but in greater quantity by the RA lymphocytes. The anti-IgG made by the RA lymphocytes was more easily absorbed by solid phase IgG than was the anti-IgG made by the normal lymphocytes and thus was judged to be of higher affinity. RA lymphocytes uninfected with EBV had higher transformation scores than did the normal controls and developed spontaneously into permanent cell lines in six instances.

Coinfection of multiple strains of Epstein-Barr virus in immunocompetent normal individuals: reassessment of the viral carrier state

Blood ◽  
2000 ◽  
Vol 95 (7) ◽  
pp. 2443-2445 ◽  
Author(s):  
Gopesh Srivastava ◽  
Kai Y. Wong ◽  
Alan K. S. Chiang ◽  
King Y. Lam ◽  
Qian Tao
Keyword(s):  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Polymerase Chain Reaction Analysis ◽  
Carrier State ◽  
Peripheral Blood Mononuclear ◽  
Barr Virus ◽  
Normal Individuals ◽  
Epstein Barr ◽  
Autopsy Specimens ◽  
Multiple Strains

Abstract This study reassesses the occurrence of Epstein-Barr virus (EBV) diversity and coinfection versus dominance of a single viral strain within immunocompetent normal carriers. Polymerase chain reaction analysis of several different polymorphic loci of the EBV genome was performed on collections of peripheral blood mononuclear cells and multiple lymphoid and epithelial tissues of the same individuals. Autopsy specimens from 15 individuals who died of causes unrelated to EBV infection served as normal viral carriers. Unexpectedly, coinfection of multiple distinct strains of EBV of the same type (usually type 1) and less frequently of both types 1 and 2 was found to be very high within individual viral carriers. These data indicate that coinfection with multiple EBV strains is much more prevalent in normal carriers than previously appreciated, which in turn has direct implications on EBV persistence, host–viral interaction and pathogenesis.

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