Open reading frame 5 of porcine reproductive and respiratory syndrome virus as a cause of virus-induced apoptosis.

Porcine reproductive and respiratory syndrome virus (PRRSV) MN-1b strain open reading frame 4 (ORF4) has been cloned, sequenced, and expressed in Escherichia coli. The homologies of nucleotide and amino acid sequences between MN-1b (US isolate) and LV (European isolate) are 69% and 64%, respectively. The data also showed that ORF4 of MN-1b is 36 bases shorter than that of LV. Western blot analysis of expressed recombinant ORF4 protein reacted with 65% (26/40) of PRRSV-infected pig sera tested. These results demonstrated that ORF4 of PRRSV may not be a well-conserved region.

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Isolation and open reading frame 5 gene analysis of porcine reproductive and respiratory syndrome virus in Yunnan Province, China

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb12.859 ◽

2012 ◽

Vol 11 (90) ◽

Author(s):

WANG Qing-lu ◽

ZHANG Shu-guang ◽

LI Fu-xiang ◽

BI Bao-liang ◽

CHAI Jun ◽

...

Keyword(s):

Yunnan Province ◽

Open Reading Frame ◽

Gene Analysis ◽

Respiratory Syndrome Virus ◽

Reading Frame

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Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 50 Represses p53-Induced Transcriptional Activity and Apoptosis

Journal of Virology ◽

10.1128/jvi.75.13.6245-6248.2001 ◽

2001 ◽

Vol 75 (13) ◽

pp. 6245-6248 ◽

Cited By ~ 28

Author(s):

Yousang Gwack ◽

Seungmin Hwang ◽

Hyewon Byun ◽

Chunghun Lim ◽

Jin Woo Kim ◽

...

Keyword(s):

Kaposi's Sarcoma ◽

Transcriptional Activity ◽

Kaposi’S Sarcoma ◽

Inhibitory Effect ◽

Open Reading Frame ◽

Creb Binding Protein ◽

Reading Frame ◽

Kaposi's Sarcoma Associated Herpesvirus ◽

Induced Apoptosis ◽

Kaposi’S Sarcoma Associated Herpesvirus

ABSTRACT Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 50 (ORF50) encodes a viral transcriptional activator which stimulates the transcription of viral early and late genes of KSHV. Here we show that ORF50 represses transcriptional activity of p53 and p53-induced apoptosis through interaction with CREB binding protein (CBP). This inhibitory effect of ORF50 on the transcriptional activity of p53 was relieved by the addition of CBP. ORF50 mutants, which are defective in interaction with CBP, lost the inhibitory effects on p53. Our data provide a framework for delineating the regulatory mechanisms used by KSHV to modulate cellular transcription and the cell cycle.

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Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System

Journal of Veterinary Science ◽

10.4142/jvs.2002.3.1.13 ◽

2002 ◽

Vol 3 (1) ◽

pp. 13 ◽

Cited By ~ 5

Author(s):

Hae Sun Jung ◽

In Wook Hwang ◽

Su Mi Kim ◽

Chul Joong Kim ◽

Kwang Soon Shin ◽

...

Keyword(s):

Semliki Forest Virus ◽

Expression System ◽

Open Reading Frame ◽

Respiratory Syndrome Virus ◽

Reading Frame ◽

Virus Expression

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Complete Genome Sequence of a Novel Subgenotype of Porcine Reproductive and Respiratory Syndrome Virus Strain JX/CH/2016, Isolated in Jiangxi, China

Genome Announcements ◽

10.1128/genomea.00980-17 ◽

2017 ◽

Vol 5 (37) ◽

Author(s):

Fanfan Zhang ◽

Deping Song ◽

Yu Ye ◽

Nannan Guo ◽

Min Zhang ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Genome Sequence ◽

Virus Strain ◽

Complete Genome Sequence ◽

Complete Genome ◽

Open Reading Frame ◽

Respiratory Syndrome Virus ◽

Reading Frame ◽

Prrsv Strain ◽

Orf5 Gene

ABSTRACT We sequenced the complete genome of porcine reproductive and respiratory syndrome virus (PRRSV) strain JX/CH/2016. Phylogenetic analysis based on the sequences of the open reading frame 5 (ORF5) gene revealed that this strain belongs to subgenotype IV. This is the first report of the complete genome sequence of PRRSV-IV.

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