scholarly journals Molecularly cloned feline immunodeficiency virus NCSU1 JSY3 induces immunodeficiency in specific-pathogen-free cats.

1996 ◽  
Vol 70 (5) ◽  
pp. 3011-3017 ◽  
Author(s):  
J S Yang ◽  
R V English ◽  
J W Ritchey ◽  
M G Davidson ◽  
T Wasmoen ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Specific Pathogen Free ◽  
Immunodeficiency Virus ◽  
Specific Pathogen

scholarly journals Experimental Mucosal Infection with Molecularly Cloned Feline Immunodeficiency Viruses

2003 ◽  
Vol 10 (1) ◽  
pp. 185-188 ◽  
Author(s):  
Mariko Kohmoto ◽  
Yasuhiro Ikeda ◽  
Eiji Sato ◽  
Yorihiro Nishimura ◽  
Yasuo Inoshima ◽  
...  
Keyword(s):  
Virus Strain ◽  
Feline Immunodeficiency Virus ◽  
Deletion Mutant ◽  
Cell Tropism ◽  
Specific Pathogen Free ◽  
Mucosal Epithelium ◽  
Immunodeficiency Virus ◽  
Mucosal Infection ◽  
Specific Pathogen

ABSTRACT Four of six specific pathogen-free cats were infected after intravaginal exposure to molecularly cloned lymphotropic but non-Crandell feline kidney (CRFK)-tropic feline immunodeficiency virus strain TM2 and its AP-1 deletion mutant. The sequences of the env V3-to-V5 region which defines the CRFK tropism were unchanged in the infected cats through the infection. These data suggest that the strain was transmitted across the mucosal epithelium without a broadening of cell tropism.

scholarly journals AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Homologous Erythrocytes as a Delivery System for Preferential Immunization with Putative Protective Antigens

1998 ◽  
Vol 5 (2) ◽  
pp. 235-241 ◽  
Author(s):  
Laura Chiarantini ◽  
Donatella Matteucci ◽  
Mauro Pistello ◽  
Umberto Mancini ◽  
Paola Mazzetti ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Vaccine Development ◽  
Ex Vivo ◽  
Degree Of Protection ◽  
Aids Vaccine ◽  
Protective Antigens ◽  
Homologous Virus ◽  
Immunodeficiency Virus ◽  
Specific Pathogen

ABSTRACT Feline immunodeficiency virus (FIV) is a useful model for testing of criteria for AIDS vaccine development. In the protocol we adopted, we used a primary isolate of FIV as a source of antigen and, for challenge, plasma from cats infected with the homologous virus never passaged in vitro. Cat erythrocytes (RBC) were coated with the surface components of freshly harvested and purified FIV by means of biotin-avidin-biotin bridges and used to immunize specific-pathogen-free cats (four doses at monthly intervals; total amount of FIV antigen administered per cat, approximately 14 μg). Immunized cats developed moderate levels of antibodies directed mainly to surface components of the virion and clearly evident lymphoproliferative responses. Four months after the last dose of immunogen, FIV-immunized cats and control cats immunized with bovine serum albumin-coated RBC were challenged. Judged from the results of the subsequent 12-month follow-up, FIV-immunized cats exhibited at least some degree of protection. However, following rechallenge, most of the FIV-immunized animals became virus positive in spite of a booster immunogen dose given 2 months before the second challenge.

scholarly journals Env-Expressing Autologous T Lymphocytes Induce Neutralizing Antibody and Afford Marked Protection against Feline Immunodeficiency Virus

2010 ◽  
Vol 84 (8) ◽  
pp. 3845-3856 ◽  
Author(s):  
Mauro Pistello ◽  
Francesca Bonci ◽  
Elisa Zabogli ◽  
Francesca Conti ◽  
Giulia Freer ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Feline Immunodeficiency Virus ◽  
Cd4 T Cells ◽  
Neutralizing Antibodies ◽  
Ex Vivo ◽  
Neutralizing Antibody ◽  
Viral Loads ◽  
Immunodeficiency Virus ◽  
Specific Pathogen

ABSTRACT The envelope (Env) glycoproteins of HIV and other lentiviruses possess neutralization and other protective epitopes, yet all attempts to induce protective immunity using Env as the only immunogen have either failed or afforded minimal levels of protection. In a novel prime-boost approach, specific-pathogen-free cats were primed with a plasmid expressing Env of feline immunodeficiency virus (FIV) and feline granulocyte-macrophage colony-stimulating factor and then boosted with their own T lymphocytes transduced ex vivo to produce the same Env and interleukin 15 (3 × 106 to 10 × 106 viable cells/cat). After the boost, the vaccinees developed elevated immune responses, including virus-neutralizing antibodies (NA). Challenge with an ex vivo preparation of FIV readily infected all eight control cats (four mock vaccinated and four naïve) and produced a marked decline in the proportion of peripheral CD4 T cells. In contrast, five of seven vaccinees showed little or no traces of infection, and the remaining two had reduced viral loads and underwent no changes in proportions of CD4 T cells. Interestingly, the viral loads of the vaccinees were inversely correlated to the titers of NA. The findings support the concept that Env is a valuable immunogen but needs to be administered in a way that permits the expression of its full protective potential.

scholarly journals Autologous and Heterologous Neutralization Analyses of Primary Feline Immunodeficiency Virus Isolates

1998 ◽  
Vol 72 (3) ◽  
pp. 2199-2207 ◽  
Author(s):  
Daniela del Mauro ◽  
Donatella Matteucci ◽  
Simone Giannecchini ◽  
Fabrizio Maggi ◽  
Mauro Pistello ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Genetic Relatedness ◽  
Neutralizing Antibody ◽  
Immunodeficiency Virus ◽  
Specific Pathogen ◽  
Considerable Individual Variation ◽  
Quasispecies Complexity ◽  
Virus Isolates ◽  
Viral Isolates ◽  
Comprehensive Study

ABSTRACT Feline immunodeficiency virus (FIV) provides a model system with which the significance of neutralizing antibody (NA) in immunosuppressive lentivirus infections may be studied. To date, no detailed analysis of the neutralization properties of primary FIV isolates has been reported. In this study, we have conducted the first comprehensive study of the sensitivity to autologous and heterologous neutralization in a lymphoid cell-based assay of 15 primary FIV isolates and, for comparison, of one tissue culture-adapted strain. Primary isolates in general proved highly NA resistant, although there was considerable individual variation. Variation was also observed in the capacity of immune sera to neutralize heterologous FIV isolates. The ability of sera to neutralize isolates or for isolates to be neutralized by sera did not correlate with epidemiological and genetic relatedness or with the quasispecies complexity of the isolates. From the study of specific-pathogen-free cats experimentally infected with viral isolates associated with NA of different breadths, it appears that the development of FIV vaccines cannot rely on the existence of viral strains inherently capable of inducing especially broad NA responses.

scholarly journals Adoptive Immunotherapy of Feline Immunodeficiency Virus with Autologous Ex Vivo-Stimulated Lymphoid Cells Modulates Virus and T-Cell Subsets in Blood

2005 ◽  
Vol 12 (6) ◽  
pp. 736-745 ◽  
Author(s):  
J. Norman Flynn ◽  
Mauro Pistello ◽  
Patrizia Isola ◽  
Lucia Zaccaro ◽  
Barbara Del Santo ◽  
...  
Keyword(s):  
T Cells ◽  
Feline Immunodeficiency Virus ◽  
Ex Vivo ◽  
Interleukin 2 ◽  
Lymphoid Cells ◽  
T Cell Subsets ◽  
Slow Increase ◽  
Immunodeficiency Virus ◽  
Vaccinia Viruses ◽  
Specific Pathogen

ABSTRACT The potential of immunotherapy with autologous virus-specific T cells to affect the course of feline immunodeficiency virus (FIV) infection was explored in a group of specific-pathogen-free cats infected with FIV a minimum of 10 months earlier. Popliteal lymph node cells were stimulated by cocultivation with UV-inactivated autologous fibroblasts infected with recombinant vaccinia viruses expressing either FIV gag or env gene products, followed by expansion in interleukin-2. One or two infusions of both Gag- and Env-stimulated cells resulted in a slow increase in FIV-specific gamma interferon-secreting T cells in the circulation of cats. In the same animals, viral set points fluctuated widely during the first 2 to 3 weeks after adoptive transfer and then returned to pretreatment levels. The preexisting viral quasispecies was also found to be modulated, whereas no novel viral variants were detected. Circulating CD4+ counts underwent a dramatic decline early after treatment. CD4/CD8 ratios remained instead essentially unchanged and eventually improved in some animals. In contrast, a single infusion of Gag-stimulated cells alone produced no apparent modulations of infection.

Laboratory Animal Research Centre (Larc) “A Specific Pathogen Free Rodent Facility” - Covid – 19 Pandemic Response Plan

2020 ◽  
Author(s):  
Muralitharan Shanmugakonar ◽  
Vijay Kanth Govindharajan ◽  
Kavitha Varadharajan ◽  
Hamda Al-Naemi
Keyword(s):  
Laboratory Animal ◽  
Animal Research ◽  
Research Centre ◽  
Functional Requirement ◽  
Specific Pathogen Free ◽  
Specific Pathogen ◽  
Response Plan ◽  
Pandemic Response ◽  
Operational Plan

Laboratory Animal Research Centre (LARC) has developed an early emergency operational plan for COVID-19 pandemic situation. Biosafety and biosecurity measures were planned and implemented ahead of time to check the functional requirement to prevent the infection. Identified necessary support for IT, transport, procurement, finance, admin and research to make the operations remotely and successfully.

Serum neutralization of feline immunodeficiency virus is markedly dependent on passage history of the virus and host system.

1994 ◽  
Vol 68 (7) ◽  
pp. 4572-4579 ◽  
Author(s):  
F Baldinotti ◽  
D Matteucci ◽  
P Mazzetti ◽  
C Giannelli ◽  
P Bandecchi ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Host System ◽  
Serum Neutralization ◽  
Immunodeficiency Virus ◽  
History Of
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