A Single Amino Acid Mutation in theCarnation Ringspot Virus Capsid Protein Allows Virion Formation but Prevents Systemic Infection
2001 ◽
Vol 75
(19)
◽
pp. 9538-9542
◽
Keyword(s):
ABSTRACT A Carnation ringspot virus (CRSV) variant (1.26) was identified that accumulates virions but is incapable of forming a systemic infection. The 1.26 capsid protein gene possesses a Ser→Pro mutation at amino acid 282. Conversion of 1.26 amino acid 282 to Ser restored systemic infection, while the reciprocal mutation in wild-type CRSV abolished systemic infection. Similar mutations introduced into the related Red clover necrotic mosaic virus capsid protein gene failed to induce the packaging but nonsystemic movement phenotype. These results provide additional support for the theory that virion formation is necessary but not sufficient for systemic movement with the dianthoviruses.
Keyword(s):
1991 ◽
Vol 72
(3)
◽
pp. 499-504
◽
1988 ◽
Vol 85
(1)
◽
pp. 170-174
◽
Keyword(s):
1988 ◽
Vol 69
(3)
◽
pp. 603-612
◽
Keyword(s):
2002 ◽
Vol 147
(7)
◽
pp. 1275-1285
◽
Keyword(s):
Keyword(s):
2013 ◽
Vol 49
(2)
◽
pp. 129-136
Keyword(s):
Keyword(s):