Nucleotide and encoded amino acid sequences of the capsid protein gene of three dengue-2 viruses isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome or dengue fever

Pathogenesis of dengue haemorrhagic fever is not fully understood, but it is thought that there is antibody enhancement during the secondary infection, which causes severe dengue haemorrhagic fever (DHF). Therefore, patients who have DHF should have a documented history of symptomatic dengue infection in the past. A retrospective descriptive-analytical study was conducted at the University Paediatric Unit at Lady Ridgeway Hospital for Children, Colombo, Sri Lanka. All children who had fulfilled the criteria for DHF admitted to the unit from April 2018 to September 2018 were recruited into the study. Relevant data were collected from bed head tickets. One hundred and eighty-four children were included in the final analysis. Thirty-three (17.9%) had a past history of documented symptomatic dengue infection, while 82.1% did not have a documented dengue infection. Twelve patients had dengue shock syndrome, and none of them had previously documented symptomatic dengue fever. Dextran was used in 96 patients in the critical phase. Twelve (42%) patients with past documented symptomatic dengue fever needed dextran while 84 (54.9%) patients without a documented past history of dengue fever needed dextran. In our clinical observation, we noticed that children with DHF mostly did not have a documented symptomatic prior dengue infection, while those with a documented symptomatic prior infection had a milder subsequent illness. In fact, the majority (82.1%) of patients with DHF did not have documented previous symptomatic dengue infection. It was also observed that the clinical course of subsequent dengue infection was less severe in patients with previously documented symptomatic dengue fever. This finding should be further evaluated in a larger scale study minimizing the all-confounding factors. This fact is more important in selecting recipients for vaccines against the dengue virus, which are supposed to produce immunity against the virus without causing the severe disease.

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Role of C- reactive proteins and liver function tests in assessing the severity of dengue fever

Journal of the Pakistan Medical Association ◽

10.47391/jpma.170 ◽

2020 ◽

pp. 1-17

Author(s):

Akash Khetpal ◽

Ansab Godil ◽

Muhammad Tanveer Alam ◽

Inam ul Haq Muhammad Makhdoom ◽

Arsalan Majeed Adam ◽

...

Keyword(s):

Liver Function ◽

Dengue Fever ◽

Dengue Shock Syndrome ◽

Liver Function Tests ◽

Dengue Haemorrhagic Fever ◽

Severe Dengue ◽

Haemorrhagic Fever ◽

C Reactive Protein ◽

Reactive Protein ◽

Function Tests

Abstract Objective: To determine whether C-reactive protein and liver function tests can serve as severity markers for dengue fever. Methods: The cross-sectional study was conducted in 2015-16 in Karachi and comprised patients with dengue fever visiting a tertiary care hospital. World Health Organisation classifications 1997 and 2009 were used to categorise patients according to clinical signs and symptoms. Receiver Operating Characteristics curve was used to determine discriminative ability and optimum cut-off value of biochemical markers. Comparisons were done through one-way analysis of variance using SPSS 17. Results: Of the 218 patients, 133(61%) were males and 85(39%) were females. The overall mean age was 35.07+15.96 years. Levels of C-reactive protein and total bilirubin were significantly higher for dengue haemorrhagic fever compared to dengue fever; dengue shock syndrome compared to dengue fever; dengue shock syndrome compared to dengue haemorrhagic fever; and dengue shock syndrome compared to dengue fever / dengue haemorrhagic fever (p<0.05 each). Levels of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase were significantly higher for dengue shock syndrome compared to dengue fever; dengue shock syndrome compared to dengue haemorrhagic fever; and dengue shock syndrome compared to dengue fever / dengue haemorrhagic fever (p<0.05 each). Levels of C-reactive protein, total bilirubin, alanine aminotransferase and alkaline phosphatise in patients with severe dengue were significantly higher compared to non-severe dengue. Conclusion: C-reactive protein and liver function tests were found to be effective biochemical markers in assessing dengue fever severity.

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A Single Amino Acid Mutation in theCarnation Ringspot Virus Capsid Protein Allows Virion Formation but Prevents Systemic Infection

Journal of Virology ◽

10.1128/jvi.75.19.9538-9542.2001 ◽

2001 ◽

Vol 75 (19) ◽

pp. 9538-9542 ◽

Cited By ~ 19

Author(s):

Tim L. Sit ◽

Patrick R. Haikal ◽

Anton S. Callaway ◽

Steven A. Lommel

Keyword(s):

Amino Acid ◽

Capsid Protein ◽

Protein Gene ◽

Systemic Infection ◽

Ringspot Virus ◽

Single Amino Acid ◽

Capsid Protein Gene ◽

Virus Capsid ◽

Virion Formation ◽

Virus Capsid Protein

ABSTRACT A Carnation ringspot virus (CRSV) variant (1.26) was identified that accumulates virions but is incapable of forming a systemic infection. The 1.26 capsid protein gene possesses a Ser→Pro mutation at amino acid 282. Conversion of 1.26 amino acid 282 to Ser restored systemic infection, while the reciprocal mutation in wild-type CRSV abolished systemic infection. Similar mutations introduced into the related Red clover necrotic mosaic virus capsid protein gene failed to induce the packaging but nonsystemic movement phenotype. These results provide additional support for the theory that virion formation is necessary but not sufficient for systemic movement with the dianthoviruses.

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Serum fatty acids and progression from dengue fever to dengue haemorrhagic fever/dengue shock syndrome

British Journal Of Nutrition ◽

10.1017/s0007114518002039 ◽

2018 ◽

Vol 120 (7) ◽

pp. 787-796 ◽

Cited By ~ 7

Author(s):

Eduardo Villamor ◽

Luis A. Villar ◽

Anyela Lozano-Parra ◽

Víctor M. Herrera ◽

Oscar F. Herrán

Keyword(s):

Fatty Acids ◽

Dengue Fever ◽

Inflammatory Responses ◽

Dengue Shock Syndrome ◽

Dengue Haemorrhagic Fever ◽

Acute Episode ◽

Severe Dengue ◽

Pentadecanoic Acid ◽

Haemorrhagic Fever ◽

Risk Of Progression

AbstractPUFA might modulate inflammatory responses involved in the development of severe dengue. We aimed to examine whether serum PUFA concentrations in patients diagnosed with dengue fever (DF) were related to the risk of progression to dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS). A secondary aim was to assess correlations between fatty acids (FA) and inflammatory biomarkers in patients with DF. We conducted a prospective case–control study nested within a cohort of patients who were diagnosed with DF and followed during the acute episode. We compared the distribution of individual FA (% of total FA) at onset of fever between 109 cases who progressed to DHF/DSS and 235 DF non-progressing controls using unconditional logistic regression. We estimated correlations between baseline FA and cytokine concentrations and compared FA concentrations between the acute episode and >1 year post-convalescence in a subgroup. DHA was positively related to progression to DHF/DSS (multivariable adjusted OR (AOR) for DHA in quintile 5v. 1=5·34, 95 % CI 2·03, 14·1;Ptrend=0·007). Dihomo-γ-linolenic acid (DGLA) was inversely associated with progression (AOR for quintile 5v. 1=0·30, 95 % CI 0·13, 0·69;Ptrend=0·007). Pentadecanoic acid concentrations were inversely related to DHF/DSS. Correlations of PUFA with cytokines at baseline were low. PUFA were lower during the acute episode than in a disease-free period. In conclusion, serum DHA in patients with DF predicts higher odds of progression to DHF/DSS whereas DGLA and pentadecanoic acid predict lower odds.

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Nucleotide sequences of the nonstructural protein NS1 gene of three dengue-2 viruses, M1, M2 and M3, isolated in Malaysia from patients with dengue haemorrhagic fever, dengue shock syndrome and dengue fever, respectively

Nucleic Acids Research ◽

10.1093/nar/18.6.1642 ◽

1990 ◽

Vol 18 (6) ◽

pp. 1642-1642 ◽

Cited By ~ 9

Author(s):

M.Y. Fong ◽

C.L. Koh ◽

S. Samuel ◽

T. Pang ◽

S.K. Lam

Keyword(s):

Dengue Fever ◽

Nonstructural Protein ◽

Dengue Shock Syndrome ◽

Nucleotide Sequences ◽

Dengue Haemorrhagic Fever ◽

Haemorrhagic Fever ◽

Ns1 Gene

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Single Amino Acid Changes in the Murine Leukemia Virus Capsid Protein Gene Define the Target ofFv1Resistance

Virology ◽

10.1006/viro.1996.0604 ◽

1996 ◽

Vol 225 (2) ◽

pp. 300-305 ◽

Cited By ~ 123

Author(s):

Christine A. Kozak ◽

Anuradha Chakraborti

Keyword(s):

Amino Acid ◽

Capsid Protein ◽

Murine Leukemia Virus ◽

Protein Gene ◽

Leukemia Virus ◽

Single Amino Acid ◽

Capsid Protein Gene ◽

Virus Capsid ◽

Virus Capsid Protein ◽

Murine Leukemia

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Isolated abducens nerve palsy – A rare clinical presentation in dengue haemorrhagic fever

Tropical Doctor ◽

10.1177/0049475520983643 ◽

2021 ◽

pp. 004947552098364

Author(s):

Sanjay KD Thakur ◽

Surabhi Dubey ◽

Somedeb Gupta

Keyword(s):

Dengue Fever ◽

Nerve Palsy ◽

Clinical Presentation ◽

Dengue Shock Syndrome ◽

Abducens Nerve ◽

Dengue Haemorrhagic Fever ◽

Abducens Nerve Palsy ◽

Haemorrhagic Fever ◽

The World ◽

Flavivirus Infection

Dengue fever is the most prevalent flavivirus infection in tropical and subtropical areas of the world. It is transmitted by the haematophagous mosquito Aedes aegypti, and it manifests clinically as classical dengue fever, dengue haemorrhagic fever and dengue shock syndrome. Rarely, dengue can be associated with neurophthalmic manifestations. We present the case of a 21-year-old woman, suffering from dengue with an isolated abducens nerve palsy.

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Occurrence and genetic diversity of pigeon circovirus strains in Poland

Acta Veterinaria Hungarica ◽

10.1556/avet.2014.004 ◽

2014 ◽

Vol 62 (2) ◽

pp. 274-283 ◽

Cited By ~ 10

Author(s):

Tomasz Stenzel ◽

Daria Pestka

Keyword(s):

Genetic Diversity ◽

Amino Acid ◽

Capsid Protein ◽

Immunosuppressive Agent ◽

Amino Acid Sequences ◽

Amino Acid Similarity ◽

Capsid Protein Gene ◽

Base Fragment ◽

Polymerase Chain ◽

Young Pigeon

Pigeon circovirus (PiCV) is an immunosuppressive agent widespread throughout the world, which causes a disease in pigeons called Young Pigeon Disease Syndrome. The aim of the study was to evaluate the prevalence of PiCV in Poland and investigate the genetic diversity relative to other known PiCV isolates. Samples from 152 pigeon flocks (88 flocks of racing pigeons and 64 flocks of fancy pigeons) from various regions of Poland were tested by polymerase chain reaction and an approximately 326-base fragment of the capsid protein gene (Cap gene) of the virus was amplified. The average viral prevalence was found to be 70.3% (76.13% in racing pigeons and 62.5% in fancy pigeons). Among the obtained positive samples, 21 were selected for sequencing and a phylogenetic analysis was performed. It was found that the majority of Polish PiCV isolates, to varying degrees, are related to isolates occurring in Europe. It was also observed that the Cap gene is variable and mutations often occur in it, which impacts the amino acid sequences in the capsid protein (nucleotide similarity averaged 86.57%, amino acid similarity averaged 89.02%).

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