In vitro studies of the binding of the ARGR proteins to the ARG5,6 promoter.
1991 ◽
Vol 11
(4)
◽
pp. 2162-2168
◽
Keyword(s):
ARGRI, ARGRII, and ARGRIII regulatory proteins control the expression of arginine anabolic and catabolic genes in Saccharomyces cerevisiae. We show here that they are also required in vitro to observe a protein-DNA complex with the promoter of the ARG5,6 gene. The specific binding of ARGR proteins in vitro is stimulated by arginine. Antibodies raised against a synthetic MCM1 polypeptide retard the migration of ARGR-DNA complex on gel mobility shift assays. This result suggests that MCM1 could be an additional regulatory element of arginine metabolism.
1991 ◽
Vol 11
(4)
◽
pp. 2162-2168
1987 ◽
Vol 7
(10)
◽
pp. 3446-3451
1993 ◽
Vol 13
(4)
◽
pp. 2586-2592
◽
1987 ◽
Vol 7
(10)
◽
pp. 3446-3451
◽
2001 ◽
Vol 183
(20)
◽
pp. 5870-5876
◽
1993 ◽
Vol 13
(4)
◽
pp. 2586-2592
2004 ◽
Vol 186
(10)
◽
pp. 3015-3021
◽
1995 ◽
Vol 15
(4)
◽
pp. 1953-1960
◽
Keyword(s):
1995 ◽
Vol 15
(11)
◽
pp. 6139-6149
◽