Template-directed primer extension catalyzed by the Tetrahymena ribozyme.
1991 ◽
Vol 11
(6)
◽
pp. 3390-3394
◽
Keyword(s):
The Tetrahymena ribozyme has been shown to catalyze an RNA polymerase-like reaction in which an RNA primer is extended by the sequential addition of pN nucleotides derived from GpN dinucleotides, where N = A, C, or U. Here, we show that this reaction is influenced by the presence of a template; bases that can form Watson-Crick base pairs with a template add as much as 25-fold more efficiently than mismatched bases. A mutant enzyme with an altered guanosine binding site can catalyze template-directed primer extension with all four bases when supplied with dinucleotides of the form 2-aminopurine-pN.
1991 ◽
Vol 11
(6)
◽
pp. 3390-3394
Keyword(s):
1993 ◽
Vol 268
(3)
◽
pp. 2195-2202
◽
2008 ◽
Vol 64
(a1)
◽
pp. C332-C332
2004 ◽
Vol 24
(24)
◽
pp. 10975-10985
◽
1978 ◽
Vol 80
(1)
◽
pp. 26-32
◽