Further Evidence That the Majority of Primary Nuclear RNA Transcripts in Mammalian Cells Do Not Contribute to mRNA
Keyword(s):
Nuclear RNA from Chinese hamster ovary cells was effectively separated into polyadenylic acid [poly(A)]-containing [poly (A) + ] and non-poly(A)-containing [poly(A) − ] fractions so that ∼90% of the poly(A) was present in the (A) + fraction. Only 25% of the 5′-terminal caps of the large nuclear molecules were present in the (A) + class, but about 70% of the specific mRNA sequences (assayed with cDNA clones) were in the (A) + class. It appears that many long capped heterogeneous nuclear RNA molecules are of a different sequence category from those molecules that are successfully processed into mRNA.
1982 ◽
Vol 2
(6)
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pp. 701-707
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1995 ◽
Vol 6
(2)
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pp. 135-150
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2009 ◽
Vol 186
(4)
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pp. 615-628
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1981 ◽
Vol 1
(2)
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pp. 179-187
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1982 ◽
Vol 2
(11)
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pp. 1459-1462
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1975 ◽
Vol 66
(3)
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pp. 577-585
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1983 ◽
Vol 3
(2)
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pp. 257-266
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