scholarly journals Sox7 Plays Crucial Roles in Parietal Endoderm Differentiation in F9 Embryonal Carcinoma Cells through Regulating Gata-4 and Gata-6 Expression

2004 ◽  
Vol 24 (23) ◽  
pp. 10492-10503 ◽  
Author(s):  
Sugiko Futaki ◽  
Yoshitaka Hayashi ◽  
Tomomi Emoto ◽  
Charles N. Weber ◽  
Kiyotoshi Sekiguchi
Keyword(s):  
Transcription Factors ◽  
Retinoic Acid ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
F9 Cells ◽  
Endoderm Differentiation ◽  
Parietal Endoderm ◽  
F9 Embryonal Carcinoma Cells ◽  
Laminin 1

ABSTRACT During early rodent development, the parietal endoderm appears from an inner cell mass and produces large amounts of basement membrane components, such as laminin-1 and collagen IV. To elucidate the regulatory network for gene expression during these procedures, we constructed a series of short interfering RNA expression vectors targeted to various transcription factors, transfected them into F9 embryonal carcinoma cells, and evaluated the effects of the gene silencing on the induction of parietal endoderm differentiation and basement membrane component production by treating F9 cells with all trans-retinoic acid and dibutyryl cyclic AMP. Among the transcription factors tested, silencing of Sox7 or combined silencing of Gata-4 and Gata-6 resulted in suppression of cell shape changes and laminin-1 production, which are the hallmarks of parietal endoderm differentiation. In cells silenced for Sox7, induction of Gata-4 and Gata-6 by retinoic acid and cyclic AMP treatment was inhibited, while induction of Sox7 was not affected in cells silenced for Gata-4 and Gata-6, indicating that Sox7 is an upstream regulatory factor for these Gata factors. Nevertheless, silencing of Sox7 did not totally cancel the action of retinoic acid, since upregulation of coup-tf2, keratin 19, and retinoic acid receptor β2 was not abolished in Sox7-silenced F9 cells. Although overexpression of Sox7 alone was insufficient to induce parietal endoderm differentiation, overexpression of Gata-4 or Gata-6 in Sox7-silenced F9 cells restored the differentiation into parietal endoderm. Sox7 is therefore required for the induction of Gata-4 and Gata-6, and the interplay among these transcription factors plays a crucial role in parietal endoderm differentiation.

scholarly journals Undifferentiated F9 embryonal carcinoma cells produce a short-chain collagen molecule

1988 ◽  
Vol 255 (1) ◽  
pp. 85-89 ◽  
Author(s):  
B D Smith ◽  
C T Baldwin
Keyword(s):  
Triple Helix ◽  
Embryonal Carcinoma ◽  
Polyacrylamide Gel Electrophoresis ◽  
Carcinoma Cells ◽  
Collagen Molecule ◽  
Embryonal Carcinoma Cells ◽  
F9 Cells ◽  
Bacterial Collagenase ◽  
Parietal Endoderm ◽  
F9 Embryonal Carcinoma Cells

The undifferentiated F9 embryonal carcinoma cells produce a unique collagen that decreases in amount during retinoic acid-induced differentiation of F9 cells into basement-membrane parietal endoderm. A bacterial-collagenase-sensitive protein of approx. 60,000 Da was resolved on polyacrylamide-gel electrophoresis. After pepsin digestion, two pepsin-resistant fragments containing hydroxyproline were demonstrated, suggesting that a portion of the molecule has a stable triple helix. The mRNA from the undifferentiated F9 cells translates a collagenase-sensitive protein with a molecular mass consistent with the 60,000 Da collagenous protein produced by undifferentiated F8 cells.

scholarly journals The Ras/Erk Pathway Induces Primitive Endoderm but Prevents Parietal Endoderm Differentiation of F9 Embryonal Carcinoma Cells

1999 ◽  
Vol 274 (3) ◽  
pp. 1487-1494 ◽  
Author(s):  
Mark H. G. Verheijen ◽  
Rob M. F. Wolthuis ◽  
Johannes L. Bos ◽  
Libert H. K. Defize
Keyword(s):  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Erk Pathway ◽  
Embryonal Carcinoma Cells ◽  
Primitive Endoderm ◽  
Endoderm Differentiation ◽  
Parietal Endoderm ◽  
F9 Embryonal Carcinoma Cells

Interaction of several related GC-box- and GT-box-binding proteins with the intronic enhancer is required for differential expression of the gb110 gene in embryonal carcinoma cells

1994 ◽  
Vol 14 (9) ◽  
pp. 5786-5793
Author(s):  
L Hamann ◽  
K U Bayer ◽  
K Jensen ◽  
K Harbers
Keyword(s):  
Transcription Factor ◽  
Binding Proteins ◽  
Embryonal Carcinoma ◽  
Regulatory Elements ◽  
Carcinoma Cells ◽  
Minor Importance ◽  
Embryonal Carcinoma Cells ◽  
Enhancer Element ◽  
F9 Cells ◽  
Parietal Endoderm

The molecular mechanisms by which expression of a gene is down-regulated after differentiation of F9 embryonal carcinoma cells into parietal endoderm-like cells was studied by characterizing the cis- and trans-regulatory elements of the gb110 gene. This gene encodes a putative RNA helicase, and its expression is down-regulated when F9 cells are differentiated with retinoic acid and cyclic AMP. The 5'-flanking region of the gene has all of the features of a GC-rich island promoter and seems to play only a minor role, if any, in the regulated expression. A 133-bp enhancer in the first intron was identified by transient chloramphenicol acetyltransferase assays that activated expression in undifferentiated F9 cells about 50- to 100-fold. As this enhancer was not active in differentiated F9 cells, it seems to be the prime mediator of the differentiation-specific down-regulation of the gb110 gene. Four different protein-binding sites, three of which contain GC- and GT-box motifs, were identified in the enhancer element. The fourth site, interacting with previously described transcription factor FTZ-F1/ELP, seems to be of minor importance for the activity of the enhancer. Mutational analysis showed that the cooperative interaction of several most likely related proteins with the three GC- and GT-box motifs was required for full enhancer activity. On the basis of their binding properties, at least two of these proteins seem to be identical or closely related to ubiquitous transcription factor Sp1. One of the GT-box-binding proteins was present in undifferentiated F9 cells but not, however, in its differentiated derivatives. The cell specificity of this transcription factor explains why the gb110 gene is not expressed or expressed only at low levels in parietal endoderm-like cells.

scholarly journals Role for up-regulated ganglioside biosynthesis and association of Src family kinases with microdomains in retinoic acid-induced differentiation of F9 embryonal carcinoma cells

Glycobiology ◽  
2005 ◽  
Vol 15 (7) ◽  
pp. 687-699 ◽  
Author(s):  
Takashige Sato ◽  
Asif Mohammad Zakaria ◽  
Satoshi Uemura ◽  
Atsushi Ishii ◽  
Yoshiko Ohno-Iwashita ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Embryonal Carcinoma ◽  
Src Family Kinases ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Induced Differentiation ◽  
Ganglioside Biosynthesis ◽  
F9 Embryonal Carcinoma Cells
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