Identification of the single gene for calmodulin in Dictyostelium discoideum.

We report the isolation and sequence determination of a cDNA containing most of the coding sequence for Dictyostelium discoideum calmodulin. The cloned cDNA was used as a probe to examine the complexity of D. discoideum genomic DNA. These studies indicated that D. discoideum cells possess a single calmodulin gene.

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Identification of the single gene for calmodulin in Dictyostelium discoideum

Molecular and Cellular Biology ◽

10.1128/mcb.6.5.1851-1854.1986 ◽

1986 ◽

Vol 6 (5) ◽

pp. 1851-1854

Author(s):

H Goldhagen ◽

M Clarke

Keyword(s):

Dictyostelium Discoideum ◽

Genomic Dna ◽

Single Gene ◽

Sequence Determination ◽

Coding Sequence ◽

Calmodulin Gene ◽

Cloned Cdna

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Improved Purification and N-Terminal Amino Acid Sequence Determination of the Contact Site A Glycoproteinof Dictyostelium discoideum

Hoppe-Seyler´s Zeitschrift für physiologische Chemie ◽

10.1515/bchm2.1982.363.2.771 ◽

1982 ◽

Vol 363 (2) ◽

pp. 771-776 ◽

Cited By ~ 15

Author(s):

Joachim STADLER ◽

Clement BORDIER ◽

Friedrich LOTTSPEICH ◽

Agnes HENSCHEN ◽

Günther GERISCH

Keyword(s):

Amino Acid ◽

Amino Acid Sequence ◽

Dictyostelium Discoideum ◽

Sequence Determination ◽

Contact Site ◽

Terminal Amino Acid ◽

Terminal Amino ◽

Terminal Amino Acid Sequence

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MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v3i1.7838 ◽

2011 ◽

Vol 3 (1) ◽

Author(s):

Lies Indah Sutiknowati

Keyword(s):

Oil Spill ◽

Deoxyribonucleic Acid ◽

Denaturing Gradient Gel Electrophoresis ◽

Gas Chromatography Mass Spectrometry ◽

Oil Degradation ◽

Sequence Determination ◽

Blast Search ◽

Degrading Bacteria ◽

Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

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