EFFECT OF CHICKEN BONE ASH ON MECHANICAL PROPERTIES OF Al 8011 REINFORCED WITH SILICON CARBIDE

To deal with demands in advanced engineering applications that focus on improved mechanical properties of metal composites, aluminium metal matrix composites are recommended. This paper deals with mechanical characteristics of aluminium (Al8011) fortified with silicon carbide (SiC) and Chicken Bone Ash (CBA).Varying percentages of silicon carbide starting from 0%, 2%, 4%, 6%, 8% and 10% with percentages of chicken bone ash starting from 10%, 8%, 6%, 4%, 2% and 0% respectively for the purpose of testing and reinforcement. Stir casting method is employed to manufacture the components. In this paper, fatigue test, impact test and hardness test is carried out to obtain results about the mechanical characteristics of the components. The mechanical characteristics observed in the resultant composites as per ASTM standards and microstructural study is used to characterize the distribution of reinforcements. Ultimately as the weight percentage of reinforcement materials increases, elevation in mechanical characteristics of material is discerned.

Chicken bone marrow mesenchymal stem cells improve lung and distal organ injury

Mesenchymal stem cells (MSCs) are associated with pulmonary protection and longevity. We separated chicken bone marrow-derived mesenchymal stem cells (BM-MSCs); investigated whether BM-MSCs can improve lipopolysaccharide (LPS)-induced lung and distal organ injury; and explored the underlying mechanisms. Ninety-six male ICR (6 weeks old) mice were randomly divided into three groups: Sham, LPS, and LPS + MSC groups. The mice were intratracheally injected with 5 mg/kg LPS to induce acute lung injury (ALI). The histopathological severity of injury to the lung, liver, kidney, heart, and aortic tissues was detected. Wet/dry ratio, protein concentrations in bronchoalveolar lavage fluid (BALF), BALF cell counts, inflammatory cytokine levels in serum, inflammatory cytokine gene expression, and oxidative stress-related indicators were detected. In addition, a survival analysis was performed in sixty male ICR mice (6 weeks old, 18–20 g). This study used chicken BM-MSCs, which are easier to obtain and more convenient than other animal or human MSCs, and have MSC-associated properties, such as a colony forming ability, multilineage differentiation potential, and certain phenotypes. BM-MSCs administration significantly improved the survival rate, systemic inflammation, and the histopathological severity of lung, liver, kidney, and aortic injury during ALI. BM-MSCs administration reduced the levels of inflammatory factors in BALF, the infiltration of neutrophils, and oxidative stress injury in lung tissue. In addition, BM-MSCs administration reduced TRL4 and Mdy88 mRNA expression during ALI. Chicken BM-MSCs serve as a potential alternative resource for stem cell therapy and exert a prominent effect on LPS-induced ALI and extrapulmonary injury, in part through TRL4/Mdy88 signaling and inhibition of neutrophil inflammation and oxidative stress injury.

Un boccone indigesto

The authors describe a case of a 9-year-old boy with oesophageal perforation and pneumomediastinum due to the ingestion of a little chicken bone.

Effect of Chicken Bone Extract Powder on Epididymal Sperm Quality of Male Wistar Rats

Calcium is one of the minerals that are essential for male reproductive function. Calcium deficiency adversely affects spermatogenesis, normal sperm function and results in infertility. The sperm quality of rats fed a standard diet containing chicken bone extract powder (BEP) was assessed in the present study. Twenty male 8-week-old rats, Wistar strain, were randomized by weight into two groups of ten rats each and fed ad libitum a standard diet containing calcium carbonate (CaCO₃, control) or chicken BEP; both were equivalent to 0.5% calcium. At the end of the 7-week consumption, the net body weight gains measured in control (101.33±21.81 g) and chicken BEP groups (100.74±26.80 g) were not significantly different (P>0.05). The in vitro sperm quality in terms of concentration, motility, viability, resistance to hypotonic stress, acrosomal reaction ability and morphology was comparable between control and chicken BEP (all were P>0.05). The results suggest that chicken BEP addition into feeds is an alternative calcium source that is as effective but less expensive as CaCO_3, a commercial calcium (fortificant). At least, it has no detrimental effect on male reproductive function.

Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine

Inactivated poultry vaccines are subject to routine potency testing for batch release, requiring large numbers of animals. The replacement of in vivo tests for cell-based alternatives can be facilitated by the identification of biomarkers for vaccine-induced immune responses. In this study, chicken bone marrow-derived dendritic cells were stimulated with an inactivated vaccine for infectious bronchitis virus and Newcastle disease virus, as well as inactivated infectious bronchitis virus only, and lipopolysaccharides as positive control, or left unstimulated for comparison with the stimulated samples. Next, the cells were lysed and subjected to proteomic analysis. Stimulation with the vaccine resulted in 66 differentially expressed proteins associated with mRNA translation, immune responses, lipid metabolism and the proteasome. For the eight most significantly upregulated proteins, mRNA expression levels were assessed. Markers that showed increased expression at both mRNA and protein levels included PLIN2 and PSMB1. Stimulation with infectious bronchitis virus only resulted in 25 differentially expressed proteins, which were mostly proteins containing Src homology 2 domains. Stimulation with lipopolysaccharides resulted in 118 differentially expressed proteins associated with dendritic cell maturation and antimicrobial activity. This study provides leads to a better understanding of the activation of dendritic cells by an inactivated poultry vaccine, and identified PLIN2 and PSMB1 as potential biomarkers for cell-based potency testing.