scholarly journals Cell Wall Assembly in Saccharomyces cerevisiae

2006 ◽  
Vol 70 (2) ◽  
pp. 317-343 ◽  
Author(s):  
Guillaume Lesage ◽  
Howard Bussey
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Critical Role ◽  
Cell Integrity ◽  
Cell Type ◽  
Cell Wall Assembly ◽  
Regulatory Dynamics ◽  
Wall Growth ◽  
Secretory System ◽  
Wall Assembly

SUMMARY An extracellular matrix composed of a layered meshwork of β-glucans, chitin, and mannoproteins encapsulates cells of the yeast Saccharomyces cerevisiae. This organelle determines cellular morphology and plays a critical role in maintaining cell integrity during cell growth and division, under stress conditions, upon cell fusion in mating, and in the durable ascospore cell wall. Here we assess recent progress in understanding the molecular biology and biochemistry of cell wall synthesis and its remodeling in S. cerevisiae. We then review the regulatory dynamics of cell wall assembly, an area where functional genomics offers new insights into the integration of cell wall growth and morphogenesis with a polarized secretory system that is under cell cycle and cell type program controls.

scholarly journals Assemblies of DivIVA Mark Sites for Hyphal Branching and Can Establish New Zones of Cell Wall Growth in Streptomyces coelicolor

2008 ◽  
Vol 190 (22) ◽  
pp. 7579-7583 ◽  
Author(s):  
Antje Marie Hempel ◽  
Sheng-bing Wang ◽  
Michal Letek ◽  
José A. Gil ◽  
Klas Flärdh
Keyword(s):  
Cell Wall ◽  
Streptomyces Coelicolor ◽  
Time Lapse ◽  
Cell Wall Assembly ◽  
Peptidoglycan Synthesis ◽  
Wall Growth ◽  
Lateral Branches ◽  
Wall Assembly ◽  
Time Lapse Imaging

ABSTRACT Time-lapse imaging of Streptomyces hyphae revealed foci of the essential protein DivIVA at sites where lateral branches will emerge. Overexpression experiments showed that DivIVA foci can trigger establishment of new zones of cell wall assembly, suggesting a key role of DivIVA in directing peptidoglycan synthesis and cell shape in Streptomyces.

The role of the exocytic pathway in cell wall assembly in yeast

Yeast ◽  
2021 ◽  
Author(s):  
Qingguo Guo ◽  
Na Meng ◽  
Guanzhi Fan ◽  
Dong Sun ◽  
Yuan Meng ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Wall Assembly ◽  
Wall Assembly ◽  
Exocytic Pathway

scholarly journals Properties of Arabinogalactan Proteins (AGPs) in Apple (Malus × Domestica) Fruit at Different Stages of Ripening

Biology ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 225
Author(s):  
Agata Leszczuk ◽  
Justyna Cybulska ◽  
Tomasz Skrzypek ◽  
Artur Zdunek
Keyword(s):  
Cell Wall ◽  
Malus Domestica ◽  
Arabinogalactan Proteins ◽  
Ex Situ ◽  
Morphological Properties ◽  
Mature Stage ◽  
Fruit Storage ◽  
Cell Wall Assembly ◽  
Wall Assembly

Arabinogalactan proteins (AGPs) are constituents of the cell wall–plasma membrane continuum in fruit tissue. The aim of the study was to characterise AGPs contained in fruit by determination of their chemical structure and morphological properties. The results were obtained from in and ex situ investigations and a comparative analysis of AGPs present in Malus × domestica fruit at different stages of ripening from green fruit through the mature stage to over-ripening during fruit storage. The HPLC and colorimetric methods were used for analyses of the composition of monosaccharides and proteins in AGPs extracted from fruit. We have found that AGPs from fruit mainly consists of carbohydrate chains composed predominantly of arabinose, galactose, glucose, galacturonic acid, and xylose. The protein moiety accounts for 3.15–4.58%, which depends on the various phases of ripening. Taken together, our results show that the structural and morphological properties of AGPs and calcium concentration in AGPs are related to the progress of ripening, which is correlated with proper fruit cell wall assembly. In line with the existing knowledge, our data confirmed the typical carbohydrate composition of AGPs and may be the basis for studies regarding their presumed properties of binding calcium ions.

scholarly journals The Role of Brachypodium distachyon Wall-Associated Kinases (WAKs) in Cell Expansion and Stress Responses

Cells ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 2478
Author(s):  
Xingwen Wu ◽  
Antony Bacic ◽  
Kim L. Johnson ◽  
John Humphries
Keyword(s):  
Cell Wall ◽  
Stress Response ◽  
Plant Cell ◽  
Stress Responses ◽  
Cell Expansion ◽  
Plant Cell Wall ◽  
Brachypodium Distachyon ◽  
Critical Role ◽  
Cell Integrity

The plant cell wall plays a critical role in signaling responses to environmental and developmental cues, acting as both the sensing interface and regulator of plant cell integrity. Wall-associated kinases (WAKs) are plant receptor-like kinases located at the wall—plasma membrane—cytoplasmic interface and implicated in cell wall integrity sensing. WAKs in Arabidopsis thaliana have been shown to bind pectins in different forms under various conditions, such as oligogalacturonides (OG)s in stress response, and native pectin during cell expansion. The mechanism(s) WAKs use for sensing in grasses, which contain relatively low amounts of pectin, remains unclear. WAK genes from the model monocot plant, Brachypodium distachyon were identified. Expression profiling during early seedling development and in response to sodium salicylate and salt treatment was undertaken to identify WAKs involved in cell expansion and response to external stimuli. The BdWAK2 gene displayed increased expression during cell expansion and stress response, in addition to playing a potential role in the hypersensitive response. In vitro binding assays with various forms of commercial polysaccharides (pectins, xylans, and mixed-linkage glucans) and wall-extracted fractions (pectic/hemicellulosic/cellulosic) from both Arabidopsis and Brachypodium leaf tissues provided new insights into the binding properties of BdWAK2 and other candidate BdWAKs in grasses. The BdWAKs displayed a specificity for the acidic pectins with similar binding characteristics to the AtWAKs.

scholarly journals Chlamydomonas reinhardtii Has Multiple Prolyl 4-Hydroxylases, One of Which Is Essential for Proper Cell Wall Assembly

2007 ◽  
Vol 19 (1) ◽  
pp. 256-269 ◽  
Author(s):  
Katriina Keskiaho ◽  
Reija Hieta ◽  
Raija Sormunen ◽  
Johanna Myllyharju
Keyword(s):  
Cell Wall ◽  
Chlamydomonas Reinhardtii ◽  
Cell Wall Assembly ◽  
Wall Assembly

Do polyamines contribute to plant cell wall assembly by forming amide bonds with pectins?

2005 ◽  
Vol 66 (21) ◽  
pp. 2581-2594 ◽  
Author(s):  
Marcello Lenucci ◽  
Gabriella Piro ◽  
Janice G. Miller ◽  
Giuseppe Dalessandro ◽  
Stephen C. Fry
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Amide Bonds ◽  
Cell Wall Assembly ◽  
Wall Assembly

Cell wall assembly ofNeurospora crassa: Isolation and analysis of cell wall-less mutants

1990 ◽  
Vol 21 (4) ◽  
pp. 233-242 ◽  
Author(s):  
Patricia Phelps ◽  
Tracey Stark ◽  
Claude P. Selitrennikoff
Keyword(s):  
Cell Wall ◽  
Cell Wall Assembly ◽  
Ofneurospora Crassa ◽  
Wall Assembly

scholarly journals Dynamics of cell wall assembly during early embryogenesis in the brown algaFucus

2016 ◽  
Vol 67 (21) ◽  
pp. 6089-6100 ◽  
Author(s):  
Thomas A. Torode ◽  
Amandine Siméon ◽  
Susan E. Marcus ◽  
Murielle Jam ◽  
Marie-Anne Le Moigne ◽  
...  
Keyword(s):  
Cell Wall ◽  
Early Embryogenesis ◽  
Cell Wall Assembly ◽  
Wall Assembly
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