scholarly journals Complete Genome Sequence of a New Ebola Virus Strain Isolated during the 2017 Likati Outbreak in the Democratic Republic of the Congo

2019 ◽  
Vol 8 (20) ◽  
Author(s):  
Tony Wawina-Bokalanga ◽  
Bert Vanmechelen ◽  
Joan Martí-Carreras ◽  
Valentijn Vergote ◽  
Kurt Vermeire ◽  
...  

Genomic sequencing for early identification of Ebola virus remains a big challenge in low-income countries. Here, we report the complete genome sequence of an Ebola virus strain obtained during the 2017 Likati outbreak in the Democratic Republic of the Congo (DRC) by using the Oxford Nanopore Technologies (ONT) MinION sequencer.

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Christiane Horwood ◽  
Sphindile Mapumulo ◽  
Lyn Haskins ◽  
Vaughn John ◽  
Silondile Luthuli ◽  
...  

Abstract Background Globally, increasing numbers of higher education institutions (HEIs) in non-English-speaking countries have adopted English as a medium of instruction (EMI), because of the perception that this provides opportunities to attract high-calibre students and academic staff, and engage with the international research community. We report an evaluation of a North–South-South collaboration to develop health research capacity in the Democratic Republic of the Congo (DRC) by establishing a postgraduate programme in nutritional epidemiology at the Kinshasa School of Public Health (KSPH), where EMI was adopted. We report experiences and perceptions of stakeholders, facilitators and students about using EMI. Methods In-depth qualitative interviews were conducted between October and December 2019 among convenience sampled stakeholders (8), facilitators (11) and students (12) involved in the programme from all three partner institutions (University of Kinshasa; University of KwaZulu-Natal, South Africa; University of Bergen, Norway). Interviews were conducted in participants’ language of preference (English or French), audio-recorded, transcribed verbatim and translated into English when required. Analysis employed a thematic approach. Results Most participants viewed EMI positively, reporting that studying in English created opportunities to access relevant literature, improve interactions with the scientific community and advance their careers. As a result of adopting EMI, some students had opportunities to present research findings at international conferences and publish their research in English. English-speaking researchers from partner institutions were able to participate in supervision of students’ research. However, inadequate English competency, particularly among students, was challenging, with some students reporting being unable to understand or interact in class, which negatively affected their academic performance. Further, EMI created barriers at KSPH among academic staff who were not proficient in English, leading to poor participation among non-English-speaking staff and lack of integration with other postgraduate programmes. Participants suggested additional English language support for EMI. Conclusion Partnerships between HEIs could be a powerful tool to develop research capacity in low-income countries in line with United Nations Sustainable Development Goals. EMI could be a solution to language barriers faced by many such partnerships, but wide-ranging support to develop English proficiency among staff and students is essential to ensure that the challenges do not outweigh the benefits.


2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Alexander N. Shvalov ◽  
Galina F. Sivolobova ◽  
Elena V. Kuligina ◽  
Galina V. Kochneva

Most of the live vaccine doses of vaccinia virus donated to the Intensified Smallpox Eradication Programme after 1971 were prepared using the L-IVP strain. A mixture of three clones of the L-IVP strain was sequenced using MySEQ. Consensus sequence similarity with the vaccinia virus Lister strain is 99.5%.


2018 ◽  
Vol 6 (16) ◽  
pp. e00316-18 ◽  
Author(s):  
Amani Ezzeddine ◽  
Nadia Soudani ◽  
Chun Kiat Lee ◽  
Ghassan Dbaibo ◽  
Husni Elbahesh ◽  
...  

ABSTRACT We report here the complete genome sequence of a human respiratory syncytial virus (HRSV) strain obtained from an infant who presented to the emergency room with an acute respiratory illness during the 2014/2015 HRSV season in Lebanon. Analysis revealed that this virus belongs to the ON1 genotype that has recently emerged worldwide.


2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Narong Nitatpattana ◽  
Kumchol Chaiyo ◽  
Supoth Rajakam ◽  
Kanya Poolam ◽  
Kusuma Chansiprasert ◽  
...  

ABSTRACT The complete genome of Zika virus (ZIKV) strain CVD_06-274 was isolated from the serum of an infected patient in Thailand in 2006. Phylogenetic analysis showed that this strain belongs to the Asian lineage and also high titers in Vero cells (RCB 10-87). It has potential for development as an inactivated ZIKV vaccine.


2019 ◽  
Vol 8 (4) ◽  
Author(s):  
Peter Thielen ◽  
Jacqueline M. Nolting ◽  
Sarah W. Nelson ◽  
Thomas S. Mehoke ◽  
Craig Howser ◽  
...  

Influenza D virus was first described in 2011 from a pig with respiratory disease; however, recent evidence indicates that cattle are the major viral reservoir. Here, we describe the genome sequence of the eighth complete swine-origin influenza D virus deposited into GenBank, D/swine/Kentucky/17TOSU1262/2017, which was collected at a 2017 swine exhibition.


2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Natsuki Tomariguchi ◽  
Kentaro Miyazaki

Rubrobacter xylanophilus strain AA3-22, belonging to the phylum Actinobacteria, was isolated from nonvolcanic Arima Onsen (hot spring) in Japan. Here, we report the complete genome sequence of this organism, which was obtained by combining Oxford Nanopore long-read and Illumina short-read sequencing data.


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