Effect of the aeration mode and yeast extract on the oxidation of high-pyrrhotite sulfide ore flotation concentrate and on the composition of the acidophilic chemolithotrophic microbial community

In order to improve the separating index of ilmenite to separate the concentrate by high intensity magnetic separation, the Heishan iron ore in Chengde did some work. Separate the sulfide ore out by flotation under the conditions of concentration by weight in feed 32%, consumption of xanthate 200g/t and 2# oil 40g/t. Use sulfate to adjust the pH of the low sulphur product of flotation, the mixture of EM351-2 and EM351-3 as foaming agent, after one roughinging and four fold concentrating, got the final titanium concentrate, the grade of which is 47.23%, and the recovery is 58.29%. During the procedure of roughing, the consumption of the reagent as follows: sulfate 2kg/t, EM351-3 1kg/t, EM351-2 900g/t. The consumption of sulfate during fourfold concentrating is 1kg/t. The index is more stable when the concentration in feed is 18%~26% during flotation. The index is stable during the continuous 26 work time for the process under this reagent system. The screen analysis of the flotation concentrate indicates that this reagent system has a better effect on the flotation of +0.074mm particles, and a bad effect on the flotation of-0.045mm particles.

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Neutrophilic Microbial Community with High Rate of Elemental Sulfur Oxidation

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1130.59 ◽

2015 ◽

Vol 1130 ◽

pp. 59-62 ◽

Cited By ~ 1

Author(s):

A.V. Belyi ◽

Tamara F. Kondrat'eva ◽

Aleksander Bulaev ◽

N.V. Solopova ◽

N.V. Grigor’eva ◽

...

Keyword(s):

Elemental Sulfur ◽

Ph Value ◽

Molecular Genetic ◽

Sulfur Oxidation ◽

High Rate ◽

Flotation Concentrate ◽

Sulfide Ore ◽

Pure Cultures ◽

Phylogenetic Identification ◽

Thermithiobacillus Tepidarius

Using molecular genetic methods, 10 species wereidentified in the community of neutrophilic microorganisms isolated from thebioreactor during the process of biooxidation of gold-arsenicpyrrhotite-bearing sulfide ore flotation concentrate. The microbial communitywas composed of chemolithotrophs oxidizing elemental sulfur and its reducedcompounds and chemoorganotrophs. The predominant S0-oxidizingchemolithotrophic strain and 3 strains of chemoorganotrophs were isolated inpure cultures. Phylogenetic identification of the pure cultures revealed thechemolithotrophic microorganism to belong to Thermithiobacillus tepidarius,while organotrophic microorganisms were identified as Parapedobacter sp.,Nocardioides nitrophenolicus, and Nocardioides sp. Themorphological and physiological characteristics of Thermithiobacillustepidarius and Nocardioides nitrophenolicus, the culturespredominant in the community of neutrophilic microorganisms, were studied.Active growth of T. tepidarius occurs within a temperature range of38–48°C at the optimal pH value of 7.0–7.5. The maximal specific growth rate ofT. tepidarius on sulfur and on solid residue which remains after theprocess of bioleaching/biooxidation of sulfide ore flotation concentrate (biooxidationresidue) was 0.13 and 0.135 h-1, respectively. The average maximalrate of S0 oxidation in the biooxidation residue was 0.107 g S0oxidizedper g S0initial h-1. The ability of Nocardioidesnitrophenolicus to destruct thiocyanate was shown.

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Changes in the Substrate Source Reveal Novel Interactions in the Sediment-Derived Methanogenic Microbial Community

International Journal of Molecular Sciences ◽

10.3390/ijms20184415 ◽

2019 ◽

Vol 20 (18) ◽

pp. 4415 ◽

Cited By ~ 3

Author(s):

Anna Szafranek-Nakonieczna ◽

Anna Pytlak ◽

Jarosław Grządziel ◽

Adam Kubaczyński ◽

Artur Banach ◽

...

Keyword(s):

Microbial Community ◽

Production Rate ◽

Yeast Extract ◽

Methane Production ◽

Biogas Production ◽

Carbon Sources ◽

Methanogenic Archaea ◽

Methane Formation ◽

Natural Environments ◽

Methane Production Rate

Methanogenesis occurs in many natural environments and is used in biotechnology for biogas production. The efficiency of methane production depends on the microbiome structure that determines interspecies electron transfer. In this research, the microbial community retrieved from mining subsidence reservoir sediment was used to establish enrichment cultures on media containing different carbon sources (tryptone, yeast extract, acetate, CO2/H2). The microbiome composition and methane production rate of the cultures were screened as a function of the substrate and transition stage. The relationships between the microorganisms involved in methane formation were the major focus of this study. Methanogenic consortia were identified by next generation sequencing (NGS) and functional genes connected with organic matter transformation were predicted using the PICRUSt approach and annotated in the KEGG. The methane production rate (exceeding 12.8 mg CH4 L−1 d−1) was highest in the culture grown with tryptone, yeast extract, and CO2/H2. The analysis of communities that developed on various carbon sources casts new light on the ecophysiology of the recently described bacterial phylum Caldiserica and methanogenic Archaea representing the genera Methanomassiliicoccus and Methanothrix. Furthermore, it is hypothesized that representatives of Caldiserica may support hydrogenotrophic methanogenesis.

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Thermoacidophilic microbial community oxidizing the gold-bearing flotation concentrate of a pyrite-arsenopyrite ore

Microbiology ◽

10.1134/s0026261714040146 ◽

2014 ◽

Vol 83 (5) ◽

pp. 539-549 ◽

Cited By ~ 8

Author(s):

A. E. Panyushkina ◽

I. A. Tsaplina ◽

N. V. Grigor’eva ◽

T. F. Kondrat’eva

Keyword(s):

Microbial Community ◽

Flotation Concentrate ◽

Gold Bearing

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Microbial community structure and function in sulfide ore bioleaching systems

Transactions of Nonferrous Metals Society of China ◽

10.1016/s1003-6326(09)60001-7 ◽

2008 ◽

Vol 18 (6) ◽

pp. 1295-1301 ◽

Cited By ~ 13

Author(s):

Guan-zhou QIU ◽

Xue-duan LIU ◽

Hong-bo ZHOU

Keyword(s):

Community Structure ◽

Microbial Community ◽

Microbial Community Structure ◽

Structure And Function ◽

Sulfide Ore ◽

And Function

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The Use of Response Surface Methodology as a Statistical Tool for the Optimisation of Waste and Pure Canola Oil Biodegradation by Antarctic Soil Bacteria

Life ◽

10.3390/life11050456 ◽

2021 ◽

Vol 11 (5) ◽

pp. 456

Author(s):

Khadijah Nabilah Mohd Zahri ◽

Azham Zulkharnain ◽

Claudio Gomez-Fuentes ◽

Suriana Sabri ◽

Khalilah Abdul Khalil ◽

...

Keyword(s):

Response Surface Methodology ◽

Microbial Community ◽

Yeast Extract ◽

Substrate Concentration ◽

Canola Oil ◽

Nitrogen Concentration ◽

Oil Biodegradation ◽

Initial Substrate ◽

Significant Factors ◽

Initial Substrate Concentration

Hydrocarbons can cause pollution to Antarctic terrestrial and aquatic ecosystems, both through accidental release and the discharge of waste cooking oil in grey water. Such pollutants can persist for long periods in cold environments. The native microbial community may play a role in their biodegradation. In this study, using mixed native Antarctic bacterial communities, several environmental factors influencing biodegradation of waste canola oil (WCO) and pure canola oil (PCO) were optimised using established one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. The factors include salinity, pH, type of nitrogen and concentration, temperature, yeast extract and initial substrate concentration in OFAT and only the significant factors proceeded for the statistical optimisation through RSM. High concentration of substrate targeted for degradation activity through RSM compared to OFAT method. As for the result, all factors were significant in PBD, while only 4 factors were significant in biodegradation of PCO (pH, nitrogen concentration, yeast extract and initial substrate concentration). Using OFAT, the most effective microbial community examined was able to degrade 94.42% and 86.83% (from an initial concentration of 0.5% (v/v)) of WCO and PCO, respectively, within 7 days. Using RSM, 94.99% and 79.77% degradation of WCO and PCO was achieved in 6 days. The significant interaction for the RSM in biodegradation activity between temperature and WCO concentration in WCO media were exhibited. Meanwhile, in biodegradation of PCO the significant factors were between (1) pH and PCO concentration, (2) nitrogen concentration and yeast extract, (3) nitrogen concentration and PCO concentration. The models for the RSM were validated for both WCO and PCO media and it showed no significant difference between experimental and predicted values. The efficiency of canola oil biodegradation achieved in this study provides support for the development of practical strategies for efficient bioremediation in the Antarctic environment.

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Specific characteristics of the strains isolated from a thermoacidophilic microbial community oxidizing antimony sulfide ore

Microbiology ◽

10.1134/s0026261710061074 ◽

2011 ◽

Vol 80 (1) ◽

pp. 70-81 ◽

Cited By ~ 2

Author(s):

A. E. Zhuravleva ◽

I. A. Tsaplina ◽

T. F. Kondrat’eva

Keyword(s):

Microbial Community ◽

Antimony Sulfide ◽

Sulfide Ore

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A peek in the micro-sized world: a review of design principles, engineering tools, and applications of engineered microbial community

Biochemical Society Transactions ◽

10.1042/bst20190172 ◽

2020 ◽

Vol 48 (2) ◽

pp. 399-409

Author(s):

Baizhen Gao ◽

Rushant Sabnis ◽

Tommaso Costantini ◽

Robert Jinkerson ◽

Qing Sun

Keyword(s):

Microbial Community ◽

Microbial Communities ◽

Environmental Remediation ◽

Real Life ◽

Design Principles ◽

Microbial Interactions ◽

Potential Applications ◽

New Gene ◽

Global Biogeochemical Cycles ◽

Synthetic Microbial Communities

Microbial communities drive diverse processes that impact nearly everything on this planet, from global biogeochemical cycles to human health. Harnessing the power of these microorganisms could provide solutions to many of the challenges that face society. However, naturally occurring microbial communities are not optimized for anthropogenic use. An emerging area of research is focusing on engineering synthetic microbial communities to carry out predefined functions. Microbial community engineers are applying design principles like top-down and bottom-up approaches to create synthetic microbial communities having a myriad of real-life applications in health care, disease prevention, and environmental remediation. Multiple genetic engineering tools and delivery approaches can be used to ‘knock-in' new gene functions into microbial communities. A systematic study of the microbial interactions, community assembling principles, and engineering tools are necessary for us to understand the microbial community and to better utilize them. Continued analysis and effort are required to further the current and potential applications of synthetic microbial communities.

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