Oxidative Decolorization of Brilliant Cresyl Blue Dye by Persulfate in the Presence of Cobalt(II) As a Catalyst in Aqueous and Micellar Media: A Kinetic Study

SummaryThe objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regulated in comparison to control group there was no statistical difference (p > 0.05) in the relative expression of MATER and ZAR1 transcripts among groups. The results indicate that the BCB dye detects immature oocyte populations with different developmental competence, although no improvement in in vitro embryo production using oocytes exposed or not to BCB was observed. Development competence of immature oocytes exposed to BCB does not seem to be associated with variations in the expression of MATER and ZAR1 transcripts.

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Study on Processing Technology of Brewer's Grains on Acid Dye Brilliant Cresyl Blue Wastewater

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.989-994.841 ◽

2014 ◽

Vol 989-994 ◽

pp. 841-844

Author(s):

Yi Hua Jiang ◽

Xin Long Jiang ◽

Cheng Gang Cai

Keyword(s):

Absorption Rate ◽

Ph Value ◽

Orthogonal Experiment ◽

Blue Dye ◽

Extraction Temperature ◽

Optimal Conditions ◽

Acid Dye ◽

Brilliant Cresyl Blue ◽

Cresyl Blue ◽

Maximal Absorption

Orthogonal experiment optimization of adsorption conditions with the factors of pH value, adsorption time, adsorbent amount of brilliant cresyl blue wastewater by brewer's grains were studied. The results showed that optimum adsorption conditions were as follows: 100 mL brilliant cresyl blue dye solution of 150 mg·L-1,adding 5.0 g·L-1brewer's grains of 60~80 mesh and adsorbed for 2.0 h,extraction temperature 30°C,pH 5.0. Under the optimal conditions, the maximal absorption rate got 95.12%.The brewer's grains is a promising, cheap, efficient, new biological materials of adsorption for brilliant cresyl blue in wastewater.

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Photoexcitation of brilliant cresyl blue dye in aqueous solution: TD-DFT study

Molecular Physics ◽

10.1080/00268976.2021.1996647 ◽

2021 ◽

Author(s):

Victor V. Kostjukov

Keyword(s):

Aqueous Solution ◽

Dft Study ◽

Blue Dye ◽

Brilliant Cresyl Blue ◽

Cresyl Blue ◽

Td Dft

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Electrical and optical properties of novel brilliant cresyl blue dye‐doped poly(methyl methacrylate) as selective cut‐off laser filters

Polymer International ◽

10.1002/pi.6082 ◽

2020 ◽

Vol 69 (12) ◽

pp. 1308-1318

Author(s):

S El‐Gamal ◽

A M Ismail

Keyword(s):

Optical Properties ◽

Methyl Methacrylate ◽

Blue Dye ◽

Electrical And Optical Properties ◽

Brilliant Cresyl Blue ◽

Poly Methyl Methacrylate ◽

Cresyl Blue

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Removal of Hazardous Brilliant Cresyl Blue Dye Utilizing Aluminum Oxide as Photocatalyst

Indonesian Journal of Chemistry ◽

10.22146/ijc.30135 ◽

2019 ◽

Vol 19 (2) ◽

pp. 292

Author(s):

Hazim Yahya Al-Gubury ◽

Hajir S. Alteemi ◽

Ali Malik Saad ◽

Rafal R. Al-Shamary

Keyword(s):

Aluminum Oxide ◽

Photocatalytic Degradation ◽

Batch Reactor ◽

Effect Of Temperature ◽

Blue Pigment ◽

Blue Dye ◽

Pseudo First Order Reaction ◽

Brilliant Cresyl Blue ◽

Air Bubble ◽

Cresyl Blue

Photocatalytic degradation of brilliant cresyl blue stain has been done by irradiating the solution pigment with a solar light in presence of aluminum oxide. The effect of important reaction has included many parameters such as catalyst mass, the initial concentration of brilliant cresyl blue dye, the effect of temperature, and initial P has been investigated in a batch reactor. All experiments are determined by usage of UV-visible spectrophotometer analyzer. The optimal concentration of catalytic has observed at 0.17 g/100 cm3 while the typical concentration of stain was recorded at 50 mg/cm3. Photocatalytic break down of shining cresyl blue pigment was favorable in the Ph 10 and 8.44 mW/cm2 light intensity. This type of degradation of the present dye study has obeyed the pseudo-first-order reaction. Photocatalytic degradation of brilliant cresyl blue dye has studied by using various concentrations of aluminum oxide. This concentration was suspended in an aqueous solution of dye which has irradiated by solar radiation in a vessel reactor at room temperature with 10 cm3/min air bubble was passed through the solution.

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Selection of developmentally competent immature equine oocytes with brilliant cresyl blue stain prior to in vitro maturation with equine growth hormone

Zygote ◽

10.1017/s096719941200072x ◽

2013 ◽

Vol 22 (4) ◽

pp. 500-504 ◽

Cited By ~ 9

Author(s):

Gabriel R. Pereira ◽

Pedro L. Lorenzo ◽

Gustavo F. Carneiro ◽

Sylvie Bilodeau-Goeseels ◽

John P. Kastelic ◽

...

Keyword(s):

Growth Hormone ◽

Oocyte Maturation ◽

Developmental Competence ◽

Epifluorescence Microscopy ◽

Blue Dye ◽

Indirect Measure ◽

Brilliant Cresyl Blue ◽

Cresyl Blue ◽

Air Atmosphere

SummaryImmature oocytes synthesize a variety of proteins that include the enzyme glucose-6-phosphate dehydrogenase (G6PDH). Brilliant cresyl blue (BCB) is a vital blue dye that assesses intracellular activity of G6PDH, an indirect measure of oocyte maturation. The objective was to evaluate the BCB test as a criterion to assess developmental competence of equine oocytes and to determine if equine growth hormone (eGH) enhanced in vitro maturation (IVM) of equine oocyte. Cumulus–oocytes complexes (COCs) were recovered by aspirating follicles <30 mm in diameter from abattoir-derived ovaries and were evaluated morphologically. Thereafter, COCs were exposed to BCB (26 μM) for 90 min at 39°C and selected based on the colour of their cytoplasm (BCB positive/BCB+ or BCB negative/BCB–). The COCs were allocated as follows: (a) IVM medium; (b) eGH group; (c) BCB–/IVM; (d) BCB+/IVM; (e) BCB–/eGH; and (f) BCB+/eGH. Then, COCs were cultured in vitro for 30 h, at 39°C in a 5%CO2 humidified air atmosphere. Cumulus-free oocytes were incubated in 10 μg/ml of bis-benzamide for 20 min at 39°C and nuclear maturation was evaluated with epifluorescence microscopy. Of the 39 COCs selected morphologically and subjected to BCB staining, 18/39 (46.2%) were classified as BCB+ and 21/39 (53.8%) as BCB– (P > 0.05). Maturation was not affected significantly by BCB classification, but the maturation rate was higher for oocytes that had been exposed to exogenous eGH versus controls (16/28, 57.1% versus 8/26, 30.8%, P < 0.05). In the present study, the BCB test was not useful for predicting competent equine oocytes prior to IVM. However, eGH enhanced equine oocyte maturation in vitro.

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